Create physiologically relevant cell culture models with Gibco Human Plasma-like Medium >

Ham's F-12K (Kaighn's) Medium This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  21127022

Related applications:

Mammalian Cell Culture

Back to top


Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.

This F-12K (Kaighn's) Medium is manufactured as follows:
• L-glutamine• HEPES
• Phenol Red

The complete formulation is available.

Using Ham's F-12K
Ham's F-12K (Kaighn's) Medium contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These additions allow the medium to be supplemented with very low levels of serum or defined components, for some cell types. Ham's F-12K (Kaighn's) Medium contains no proteins or growth factors, and is therefore often supplemented with growth factors and Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell line. Ham's F-12K (Kaighn's) Medium uses a sodium bicarbonate buffer system (2.5 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Dual-site cGMP manufacturing
For supply chain continuity, we manufacture Ham's F-12K (Kaighn's) Medium at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
For Research Use Only. Not for use in diagnostic procedures.


Shipping Condition: Room Temperature
Volume (Metric): 500 ml
Product Line: Gibco™
Form: Liquid
Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Green Features: Sustainable packaging
Quantity: 500mL
Glucose: Low Glucose
Sodium Pyruvate Additive: Sodium Pyruvate

Contents & storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient