Thermo Scientific™

PageRuler™ Unstained High Range Protein Ladder

Catalog number:  26637

Related applications:

Protein Biology

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Thermo Scientific PageRuler Unstained High Range Protein Ladder is a mixture of eight (8) unstained proteins (60 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Features of PageRuler Unstained High Range Protein Ladder:

Size range—8 proteins spanning 60 to 250kDa
Ready-to-use—supplied in a loading buffer for direct loading on gels
Sharp bands—excellent accuracy for detection in with coomassie or silver stains
Reference band—150kDa band has greater intensity for easy orientation
Tagged—each protein in the ladder contains an integral Strep-tag™ II Sequence
Quality tested—each lot evaluated by SDS-PAGE and Western blotting

The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 150kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained High Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.

• Proteins (0.02 to 0.05 mg/mL of each) in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol

• Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
For Research Use Only. Not for use in diagnostic procedures.


Number of Markers: 8
Product Line: PageRuler™
Recommended Applications: Tris-acetate gels
Size Range: 60 to 250 kDa
System Type: SDS-PAGE
Molecular Weight: 250, 200, 150, 120, 100, 85, 70, 60 kDa
Stain Type: Unstained
Quantity: 2 x 250 mL

Contents & storage

Upon receipt store at -20°C. Product is shipped with an ice pack.