Pierce™ High Capacity Streptavidin Chromatography Cartridge, 5 mL
Thermo Scientific™
Pierce™ High Capacity Streptavidin Chromatography Cartridge, 5 mL
The Thermo Scientific™ PierceTM High Capacity Streptavidin Chromatography Cartridges are convenient, ready-to-use devices for separating biotinylated molecules from non-biotinylated moleculesRead more
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Catalog NumberQuantitySufficient For
877401 CartridgeBinding ≥/= 500 μg Biotin per Cartridge (≥/= 50 mg Biotinylated BSA per Cartridge)
877392 CartridgesBinding ≥/= 100 μg Biotin per Cartridge (≥/= 10 mg Biotinylated BSA per Cartridge)
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Catalog number 87740
Price (USD)/ 1 x 5 mL
390.00
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Quantity:
1 Cartridge
Sufficient For:
Binding ≥/= 500 μg Biotin per Cartridge (≥/= 50 mg Biotinylated BSA per Cartridge)
Request bulk or custom format
Price (USD)/ 1 x 5 mL
390.00
Add to cart
Pierce™ High Capacity Streptavidin Chromatography Cartridge, 5 mL
Catalog number87740
Price (USD)/ 1 x 5 mL
390.00
-
Add to cart
The Thermo Scientific™ PierceTM High Capacity Streptavidin Chromatography Cartridges are convenient, ready-to-use devices for separating biotinylated molecules from non-biotinylated molecules and for purifying antigens using biotinylated antibodies. The chromatography cartridges contain a beaded resin of immobilized recombinant streptavidin protein that binds biotinylated proteins at greater than 10 mg/mL of beads. This corresponds to 2 to 3 times higher biotin-binding capacity than products from other suppliers and is ideal when a high amount of recovery is required.

These cartridges enable fast, easy, and reproducible chromatographic separations and are compatible with the major automated liquid-chromatography systems or manual syringe processing (see Table 1 for cartridge general properties). The cartridges attach directly to ÄKTATM or FPLC systems without additional connectors. The included accessory pack readily adapts cartridges for use with Luer-LokTM syringe fittings or 1/16' tubing. These cartridges can be regenerated multiple times for affinity purification.

Features of High Capacity Streptavidin Agarose:
Streptavidin—purified recombinant streptavidin protein (53 kDa, near-neutral pI); tetrameric with four biotin-binding site per molecule
Agarose resin—support is cross-linked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable—streptavidin is immobilized by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
High capacity—this variety of beads has a dense load of immobilized streptavidin, providing a binding capacity greater than 10 mg of biotinylated BSA per mL of resin
Two formats—available in several bottle sizes of resin slurry and as packed 1 mL and 5 mL chromatography cartridges

Properties of cross-linked 6% beaded agarose (CL-6B):
• Support pH stability: 2 to 14 (short term); 3 to 13 (long term)
• Average particle size: 45 to 165 microns
• Exclusion limit: 10,000 to 4,000,000 daltons
• Maximum recommended flow rate: 4 mL/minute (1 mL column), 5 mL/min (5 mL column)
• Maximum linear velocity: 30 cm per hour
• Maximum pressure: 0.3 MPa, 43.5 psi or 3 bar

Pierce High Capacity Streptavidin Chromatography Cartridges consist of purified recombinant streptavidin covalently immobilized at a high density onto high-quality cross-linked 6% beaded agarose. Biotinylated antibodies, proteins, peptides, nucleic acids, and other molecules or interaction complexes can be captured, immunoprecipitated, removed, or purified from samples using this streptavidin resin.

Streptavidin is a tetrameric protein containing four biotin-binding sites similar to avidin. The native protein from Streptomyces avidinii is carbohydrate-free, has an acidic pI of 5.5 and a mass of 75 kDa. The recombinant protein used to manufacture Pierce streptavidin resins has a near-neutral isoelectric point and a mass of 53 kDa. Streptavidin generally has less nonspecific binding than avidin (from chicken egg white) because of the absence of carbohydrates.

For more information see: Chromatography Cartridges for Desalting and Affinity Purification

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Capacity (Metric)5 mL
Particle Size45 to 165 μm
Product LinePierce™
Quantity1 Cartridge
Protein FormRecombinant
TargetBiotin-Tagged Molecule
TypeChromatography Cartridge
Stationary PhaseStreptavidin
Sufficient ForBinding ≥/= 500 μg Biotin per Cartridge (≥/= 50 mg Biotinylated BSA per Cartridge)
FormatChromatography Cartridge
Column TypeAffinity, Agarose Resin
Unit Size1 x 5 mL
Contents & Storage
Upon receipt store at 4°C. Do not freeze the resin. Product is shipped at ambient temperature.