The Total Reactive Oxygen Species (ROS) Assay Kit 520 nm contains the necessary regent and buffer for identifying ROS in cells by flow cytometry in the FITC channel. Reactive oxygen species are chemically reactive oxygen-containing molecules that are generated as a natural byproduct of the oxygen metabolism. ROS are constantly generated and eliminated under normal physiologic conditions and have important functions in cell signaling, homeostasis, and clearance of microbial infections. During times of environmental stress such as exposure to UV or heat, or during infection, ROS levels can increase dramatically leading to oxidative stress. Oxidative stress can result in damage to cellular proteins, lipids, and DNA, and has been linked to cardiovascular disease, cancer, diabetes, inflammation, and aging. Examples of ROS include superoxide ions and peroxides.

Reported Application
Flow Cytometric Analysis