ROS-Nitric Oxide Pathway Reagents and Kits

CM-H2DCFDA (General Oxidative Stress Indicator) Invitrogen™

CM-H2DCFDA is a chloromethyl derivative of H2DCFDA, useful as an indicator for reactive oxygen species (ROS) in cells. This indicator exhibits much better retention in live cells than H2DCFDA. CM-H2DCFDA passively diffuses into cells, where its acetate groups are cleaved by intracellular esterases...

CellROX™ Deep Red Reagent, for oxidative stress detection Invitrogen™

CellROX™ Deep Red Reagent is a novel fluorogenic probe for measuring cellular oxidative stress in both live and fixed cell imaging, with absorption/emission maxima at ∼644/665 nm. The cell-permeant dye is non-fluorescent while in a reduced state, and exhibits bright fluorescence upon oxidation by...

Total Reactive Oxygen Species (ROS) Assay Kit 520 nm Invitrogen™

The Total Reactive Oxygen Species (ROS) Assay Kit 520 nm contains the necessary regent and buffer for identifying ROS in cells by flow cytometry in the FITC channel. Reactive oxygen species are chemically reactive oxygen-containing molecules that are generated as a natural byproduct of the oxygen...

CellROX™ Green Reagent, for oxidative stress detection Invitrogen™

CellROX™ Green Reagent is a novel fluorogenic probe for measuring oxidative stress in live cells. The cell-permeant dye is weakly fluorescent while in a reduced state and exhibits bright green photostable fluorescence upon oxidation by reactive oxygen species (ROS) and subsequent binding to DNA,...

H2DCFDA (H2-DCF, DCF) Invitrogen™

The cell-permeant 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) (also known as dichlorofluores cin diacetate) is a chemically reduced form of fluorescein used as an indicator for reactive oxygen species (ROS) in cells, for example to detect the generation of reactive oxygen intermediates in...

MitoSOX™ Mitochondrial Superoxide Indicators, for live-cell imaging Invitrogen™

MitoSOX Green and MitoSOX Red superoxide indicators are novel fluorogenic dyes specifically targeted to mitochondria in live cells. Oxidation of the MitoSOX reagent by mitochondrial superoxide produces bright green or red fluorescence.

CellROX™ Reagent Variety Pack, for oxidative stress detection Invitrogen™

The CellROX™ Variety Pack contains one vial (50 µL) each of CellROX™ Green Reagent, CellROX™ Orange Reagent, and CellROX™ Deep Red Reagent. These reagents are novel fluorogenic probes for measuring cellular oxidative stress in live cells.

CellROX™ Green Flow Cytometry Assay Kit Invitrogen™

The CellROX™ Green Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Green Reagent, as well as SYTOX™ Red Dead Cell Stain, N-acetyl cysteine (an antioxidant, for negative control), and...

BODIPY™ 665/676 (Lipid Peroxidation Sensor) Invitrogen™

Lipid peroxidation can be detected with the lipophilic probe, BODIPY™ 665/676 dye. This probe exhibits a change in fluorescence after interaction with peroxyl radicals.

BODIPY™ 581/591 C11 (Lipid Peroxidation Sensor) Invitrogen™

BODIPY™ 581/591 undecanoic acid can be used to detect reactive oxygen species (ROS) in cells and membranes. Oxidation of the polyunsaturated butadienyl portion of the dye results in a shift of the fluorescence emission peak from ∼590 nm to ∼510 nm.

Nitric Oxide Assay Kit Invitrogen™

The Nitric Oxide Assay Kit determines nitric oxide composition through measurement of nitrate (NO3) and nitrite (NO2) levels in urine, saliva, plasma, serum, and other biological fluids. Characteristics of the Nitric Oxide Assay Kit: Target: nitric oxide and relative levels of nitrite and nitrate...

Image-IT™ LIVE Green Reactive Oxygen Species Detection Kit, for microscopy Invitrogen™

The Image-iT™ LIVE Green Reactive Oxygen Species (ROS) Detection Kit provides the key reagents necessary for the detection of ROS in live cells. The assay is based on 5-(and-6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA), a reliable fluorogenic marker for ROS in live cells.

Singlet Oxygen Sensor Green - Special Packaging Invitrogen™

Singlet Oxygen Sensor Green is a detection reagent that is highly selective for singlet oxygen. Unlike other available fluorescent and chemiluminescent singlet oxygen detection reagents, Singlet Oxygen Sensor Green does not show any appreciable response to hydroxyl radical or superoxide.

Dihydroethidium (Hydroethidine) Invitrogen™

The superoxide indicator dihydroethdium, also called hydroethidine, exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red.

Dihydrorhodamine 123 Invitrogen™

Dihydrorhodamine 123 is an uncharged and nonfluorescent reactive oxygen species (ROS) indicator that can passively diffuse across membranes where it is oxidized to cationic rhodamine 123 which localizes in the mitochondria and exhibits green fluorescence.