Applied Biosystems™

Absolute Q™ 1-step RT-dPCR Master Mix (4X)

Catalog number: A55146
Applied Biosystems™

Absolute Q™ 1-step RT-dPCR Master Mix (4X)

Catalog number: A55146
Catalog Number
A55146
Unit Size
200 reactions
Price (USD)
Price: 1,100.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A55146200 reactions
Price: 1,100.00
Your Price:
***
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X) is optimized for use with the QuantStudio Absolute Q Digital PCR System in a simple workflow with minimal processing steps. The 4X formulation enables analysis of higher sample volumes and facilitates accurate quantification of RNA targets without using a standard curve.

Features of Absolute Q 1-step RT-dPCR Master Mix include:
Fast time-to-results—5 minutes of hands-on time and RT-dPCR run time of ∼65 minutes
Simple workflow—set up the reaction like 1-Step RT-qPCR, then load and run the plate in a single instrument
High sensitivity—ability to detect <1 copy/uL of a specific target within a 4-plex reaction
Optimized for multiplexing—detect up to four targets in a single reaction
Reproducibility—highly reproducible results between technical replicates; consistently generate >20,000 analyzable micro-chambers

Simple and efficient workflow
The Absolute Q system simplifies the entire dPCR workflow. Prepare the dPCR reaction mix as you would for qPCR, pipette the reaction mix and isolation buffer into the microfluidic array plate (MAP), and place the plate in the QuantStudio Absolute Q Digital PCR System. Compartmentalization, thermal cycling, and data acquisition of up to four targets occur in a single instrument.

High sensitivity when multiplexing
Absolute Q 1-step RT-dPCR Master Mix (4X) can detect <1 copy/uL of a target while amplifying three additional targets in the same reaction. The Absolute Q system enables analysis of >95% of the loaded reaction to help ensure detection of rare targets.

High reproducibility
Absolute Q 1-step RT-dPCR Master Mix (4X) is formulated to consistently generate >20,000 analyzable micro-chambers and help deliver highly reproducible results between replicates.

For research use only. Not for use in diagnostic procedures.

Specifications

Compatibility
Absolute Q Digital PCR System
Detection Method
Primer-Probe
Format
dPCR Master Mix
Hot Start
Hot Start Taq Polymerase
No. of Reactions
200 Reactions
PCR Method
Digital PCR
Polymerase
Taq Polymerase
Reaction Speed
Results in less than 2 hours
Sample Type
RNA
Technique
Digital PCR
Volume
450 µL
Speed
Results in less than 2 hours