Use Reinforced Clostridial Agar for the isolation and cultivation of anaerobic organisms occurring in a variety of habitats, including food and pathological specimens.

Reinforced Clostridial Agar is a solid version of Thermo Scientific Oxoid Reinforced Clostridium Medium suitable for the cultivation and enumeration of clostridia and other anaerobes. Reinforced Clostridial Agar does not differ significantly in performance from the pork-starch-pea agar of Anderson¹for the count of anaerobes.

It is employed for the estimation of clostridia in food—see below (also Barnes et al.² and Angelotti³). Attenborough and Scarr⁴employed RC Agar, in conjunction with membrane filters, for the count of Clostridium thermosaccharolyticum in sugar.

Reinforced Clostridial Agar is also frequently employed for the investigation of intestinal flora: Perry et al.⁵for investigation of bovine rumen streptococci; Williams Smith & Crabb⁶ used the Oxoid medium with added sodium chloride or blood for counts on human or animal feces; Barnes & Goldberg⁷ employed the Oxoid medium with added chlortetracycline hydrochloride or sodium azide and ethyl violet, for the examination of poultry feces; the Oxoid medium was also used by Goldberg et al.⁸ for the examination of poultry fecal samples; Williams Smith⁹ employed Oxoid Reinforced Clostridial Agar with added blood for the ‘total’ and Lactobacillus count of human and animal feces; with added blood and neomycin for determination of Bacteroides, anaerobic Gram-negative cocci, ‘total’ streptococci, sporeformers, yeasts, and ‘actino’ types.

Sneath¹⁰ used Oxoid RC Agar and other media for the anaerobic count of microorganisms from soil samples up to approximately 300 years old. Gregory et al.¹¹ also employed the Oxoid medium for the estimation of anaerobes in molding hay.

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