Invitrogen™
ChargeSwitch™ Forensic DNA Purification Kit
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Description
ChargeSwitch® Forensic DNA Purification Kit uses magnetic bead technology to allow for rapid and efficient purification of genomic DNA from a variety of forensic samples, including poor or environmentally degraded samples, and is fully validated for Single Tandem Repeat (STR) analysis.• Optimized for higher-purity DNA extraction
• Validated for STR profiling on diverse forensic samples-set
• Compatible with high-throughput protocols
Rapid, Three Step, pH Based Purification Protocol
ChargeSwitch® Forensic DNA Purification Kit allows purification of genomic DNA typically in less than 15 minutes following sample preparation and lysis, using magnetic beads with a unique, ionizable, nucleic acid-binding ligand whose charge can be switched based on the pH of the surrounding medium. Purification is achieved through a simple three-step procedure in aqueous buffers and avoids the use of guanidine, ethanol, and other troublesome reagents.
Validated for Single Tandem Repeat (STR) Profiling
ChargeSwitch® Forensic DNA Purification Kit is suitable for use in STR analysis, for mitochondrial DNA (mtDNA) extraction from hair follicles for single nucleotide polymorphism (SNP) analysis, and includes a flexible protocol to facilitate genomic DNA isolation from a variety of forensic sample types (Figure 1), such as:
• Dried blood spots on paper and clothing
• Hair follicles and hair shafts
• Cigarette butts
• Cigarette paper
• Envelopes
• Drinking vessel swabs
• Chewing gum
• Sperm head cells
• Vaginal epithelial cells
• Door handle swabs
• Strip removed cells (e.g., hats, coats, and gloves)
• "Touch DNA" (e.g., tools, mobile phones, and microscopes)
• Dyed denim
Compatible with Single Tube and High-Throughput Protocols
ChargeSwitch® Forensic DNA Purification Kit is designed for automated processing of both small numbers of samples individually, as well as large numbers of samples using a liquid handling robot or a multi-channel pipette.
For processing up to 24 individual samples at a time the MagnaRack™ two-piece magnetic separation tube rack will be required. This rack consists of a magnetic base station and a removable tube rack, which can hold up to 24 microcentrifuge tubes and can be used with most ChargeSwitch® products.
For high-throughput applications utilizing 96 well blocks, the 96-Well Magnetic Separator will be required. This rack can hold and separate up to 96 magnetic bead samples in a deep well plate when placed on top of the magnetic base station.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Sample Type (General): | Forensic Samples |
|---|---|
| Isolation Technology: | ChargeSwitch® Chemistry |
| Downstream Application: | Forensic Testing, PCR |
| High Throughput Compatibility: | High Throughput-Compatible |
| Product Line: | ChargeSwitch® |
| Product Size: | 100 preps |
| Shipping Condition: | Room Temperature |
Contents & storage
All components of the ChargeSwitch® Forensic DNA Purification Kits are shipped at room temperature. Upon receipt, store the Proteinase K at 4“C. Store all other components at room temperature. All components are guaranteed stable for 6 months, if stored properly.
Components:
ChargeSwitch® Lysis Buffer (L13)
ChargeSwitch® Magnetic Beads
Proteinase K (20 mg/ml in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
ChargeSwitch® Purification Buffer (N5)
ChargeSwitch® Wash Buffer (W12)
ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Components:
ChargeSwitch® Lysis Buffer (L13)
ChargeSwitch® Magnetic Beads
Proteinase K (20 mg/ml in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
ChargeSwitch® Purification Buffer (N5)
ChargeSwitch® Wash Buffer (W12)
ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
