For PCR enzyme with ≤0.01 copy of bacterial gDNA per enzyme unit, check out Invitrogen Platinum Taq DNA Polymerase, DNA-free.
Thermo Scientific™
Taq DNA Polymerase, native, without BSA (5 U/µL)
Description
Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E. coli.Highlights
• Thermostable—half life is more than 40 min at 95°C.
• Generates PCR products with 3'-dA overhangs.
• Supplied with two buffers—10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).
Applications
• Routine PCR amplification of DNA fragments up to 5 kb
• DNA labeling (see Reference s 1-3)
• High throughput PCR
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Hot Start: | No |
|---|---|
| Includes: | 2 x 1mL of 25mM MgCl2, 2 x 1mL of 10X Taq Buffer with KCl, 2 x 1mL of 10X Taq Buffer with (NH4)2SO4 |
| Fidelity (vs. Taq): | 1 X |
| GC-Rich PCR Performance: | Low |
| Overhang: | 3'-A |
| Polymerase: | Taq DNA Polymerase |
| Reaction Format: | Standalone |
| Reaction Speed: | Standard |
Contents & storage
Contains:
• 100 µL Taq DNA Polymerase, native (5 U/µL)
• 2 x 1.25 mL 10X Taq Buffer with KCl
• 2 x 1.25 mL 10X Taq Buffer with (NH4)2SO4
• 2 x 1.25 mL 25 mM MgCl2
Store at -20°C.
• 100 µL Taq DNA Polymerase, native (5 U/µL)
• 2 x 1.25 mL 10X Taq Buffer with KCl
• 2 x 1.25 mL 10X Taq Buffer with (NH4)2SO4
• 2 x 1.25 mL 25 mM MgCl2
Store at -20°C.
