For PCR enzyme with ≤0.01 copy of bacterial gDNA per enzyme unit, check out Invitrogen Platinum Taq DNA Polymerase, DNA-free.
Thermo Scientific™

Taq DNA Polymerase, native, without BSA (5 U/µL)

Catalog number:  EP0282

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Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E. coli.


Thermostable—half life is more than 40 min at 95°C.
Generates PCR products with 3'-dA overhangs.
Supplied with two buffers—10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.
Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).


• Routine PCR amplification of DNA fragments up to 5 kb
• DNA labeling (see Reference s 1-3)
• High throughput PCR
For Research Use Only. Not for use in diagnostic procedures.


Hot Start: No
Includes: 2 x 1mL of 25mM MgCl2, 2 x 1mL of 10X Taq Buffer with KCl, 2 x 1mL of 10X Taq Buffer with (NH4)2SO4
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Overhang: 3'-A
Polymerase: Taq DNA Polymerase
Reaction Format: Standalone
Reaction Speed: Standard

Contents & storage

• 100 µL Taq DNA Polymerase, native (5 U/µL)
• 2 x 1.25 mL 10X Taq Buffer with KCl
• 2 x 1.25 mL 10X Taq Buffer with (NH4)2SO4
• 2 x 1.25 mL 25 mM MgCl2

Store at -20°C.