Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E. coli.
Highlights
• Thermostable—half life is more than 40 min at 95°C. • Generates PCR products with 3'-dA overhangs. • Supplied with two buffers—10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming. • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).
Applications
• Routine PCR amplification of DNA fragments up to 5 kb • DNA labeling (see Reference s 1-3) • High throughput PCR
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection Method
Primer-probe
GC-Rich PCR Performance
Low
Polymerase
Taq DNA Polymerase
Reaction Speed
Standard
Product Type
Taq DNA Polymerase
For Use With (Application)
Standard PCR
Concentration
10X
Fidelity (vs. Taq)
1 X
Hot Start
No
Overhang
3'-A
Reaction Format
Standalone
Starting Material
DNA
Volume
2 mL
Contents & Storage
Contains: • 100 μL Taq DNA Polymerase, native (5 U/μL) • 2 x 1.25 mL 10X Taq Buffer with KCl • 2 x 1.25 mL 10X Taq Buffer with (NH4)2SO4 • 2 x 1.25 mL 25 mM MgCl2