Thermo Scientific™

Phusion Green High-Fidelity DNA Polymerase (2 U/µL)

Catalog number:  F534S

Related applications:

PCR & Real-Time PCR

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Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

The Phusion Green format is a combination of Phusion High-Fidelity DNA Polymerase with 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The density reagent and dyes do not interfere with excellent performance of Phusion DNA Polymerases and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.


• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available as a Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)


• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at

For Research Use Only. Not for use in diagnostic procedures.


Fidelity (vs. Taq): 52 X
GC-Rich PCR Performance: High
Hot Start: No
Overhang: Blunt
Polymerase: Phusion High-Fidelity DNA Polymerase
Product Line: Phusion
Quantity: 100U
Reaction Format: Standalone
Reaction Speed: Fast

Contents & storage

• Phusion DNA Polymerase, 2 U/µL
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• 50 mM MgCl2

Both Phusion Green HF and Phusion Green GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.