The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris. The vectors carry the HIS4 gene for selection of transformants on histidine-deficient medium. The Original Pichia Expression Kit provides all of the tools and reagents needed to express a gene of interest from the AOX1 promoter.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Bacterial or Yeast Strain
GS115, KM71
Cloning Method
Restriction Enzyme⁄ MCS
Cell Type
Yeast Cells
Format
Kit
Promoter
AOX1
Species
P. pastoris
Vector
pPIC
Expression Mechanism
Cell-Based Expression
Expression System
Yeast
For Use With (Application)
Protein Expression
Product Type
Expression Kit
Protein Tag
Untagged
Quantity
1 Kit
Contents & Storage
The Original Pichia Expression Kit includes 10 μg each of pHIL-D2 and pPIC3.5 vectors for intracellular expression and pHIL-S1 and pPIC9 vectors for secreted expression, Pichia pastoris strains GS115 (his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, media (YP and YNB), Spheroplast Transformation Kit, and 2 μg each 5´ AOX1, 3´ AOX1, and α-factor sequencing primers. Store vectors and primers at -20°C. Store strains at room temperature. All components are guaranteed stable for 6 months when properly stored.