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GeneBLAzer® HCRTR2-Gα15-NFAT-bla CHO-K1 Cells

Catalog number: K1737

 Related applications: Target & Lead Identification & Validation

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Description

GeneBLAzer® HCRTR2-Gα15-NFAT-bla CHO-K1 cells contain the human Orexin Receptor B (HCRTR2) stably integrated into the GeneBLAzer® Gα15-NFAT-bla CHO-K1 cell line. GeneBLAzer® Gα15-NFAT-bla CHO-K1 cells (Cat. no. K1537) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Promiscuous G protein, Gα15.

The GeneBLAzer® HCRTR2-Gα15-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of Orexin B. In addition, GeneBLAzer® HCRTR2-Gα15-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The HCRTR2 receptor has been detected in the brain as well as adrenal gland, gastrointestinal tract, and pancreas. The signaling cascades used by orexin receptors appear to be varied and cell type dependant. In CHO cells, calcium release is seen after agonist treatment. In hypothalamic neurons, the frequency of action potentials is increased. Administering either orexin peptide into the bloodstream of rats caused an increase in feeding behavior. The orexin system is also thought to regulate neuroendocrine function and the sleep wake cycle. HCRTR2 has been directly implicated in narcolepsy in animal studies.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: GeneBLAzer®
Reporter: BLA (Beta-Lactamase)
Cell Line: CHO-K1
Technique: FRET
Cell State: Dividing Cells
Gene Symbol: HCRTR2
Product Size: 2 x 106 cells
Accession Number: NM_001526.2
Detection Method: Fluorescent
Druggable Target: GPCRs
Population Status: Single Cell Clone Origin

Contents & storage

Contents:
K1737 GeneBLAzer® HCRTR2-Gα15-NFAT-bla CHO-K1 cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer® HCRTR2-Gα15-NFAT-bla CHO-K1 cells
• GeneBLAzer® HCRTR2-Gα15-NFAT-bla CHO-K1 Assay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer® HCRTR2-Gα15-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

Documents

Manuals & protocols