Invitrogen™

PureLink™ HiPure Plasmid Midiprep Kit

Catalog number: K210005
Invitrogen™

PureLink™ HiPure Plasmid Midiprep Kit

Catalog number: K210005
Catalog Number
K210005
also known as K2100-05
Unit Size
50 preps
Price (USD)
Price: 554.00
Your Price:
Availability
***
Quantity
Catalog Number
K210004
also known as K2100-04
Unit Size
25 preps
Price (USD)
Price: 298.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K210005
also known as K2100-05
50 preps
Price: 554.00
Your Price:
***
K210004
also known as K2100-04
25 preps
Price: 298.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
The PureLink™ HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Midiprep Kit protocol will typically yield 100–350 µg plasmid DNA from 15–25 mL bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients. Advantages of the PureLink™ HiPure Plasmid Midiprep Kit include:

Labor and time saving—no need for extra steps to remove endotoxin and other contaminants
High-purity, low-endotoxin product—pure enough for mammalian cell transfection
Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs

Anion-exchange chromatography for plasmid purification
The PureLink™ HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients. The resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal. Plasmid preparations can typically be completed in less than 2 hours.

Contaminant-free DNA
Unlike cesium chloride gradient protocols, PureLink™ HiPure Plasmid Purification Systems do not use organic solvents, ethidium bromide, or cesium chloride, all of which are troublesome to work with and to dispose of. Typically, plasmid DNA prepared using the PureLink™ HiPure Plasmid Midiprep Kit will have an A260/A280 ratio of >1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications. Endotoxin levels are typically within 0.1–1 EU/µg, making this plasmid DNA ideal for mammalian cell transfection.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Column
Isolation Technology
Anion Exchange Resin with Lysate Clearing Filter
Sample Type
Bacterial Culture
Elution Volume
5 ml
Final Product Type
BAC DNA, Plasmid DNA
For Use With (Application)
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Includes
  • 200mL Resuspension Buffer (R3)
  • 1.5mL RNase A
  • 200mL Lysis Buffer (L7)
  • 200mL Precipitation Buffer (N3)
  • 2 × 250mL Equilibration Buffer (EQ1)
  • 2 × 500mL Wash Buffer (W8)
  • 250mL Elution Buffer (E4)
  • 30mL TE Buffer
  • 50 HiPure Columns
  • 10 Column Holders
No. of Reactions
50 Preps
Plasmid
<40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Prep Scale
100-200 µg (Medium-Scale) Plasmid DNA
Product Line
PureLink™
Product Type
Plasmid MidiPrep Kit
Quantity
50 Preps
Purity
Transfection Grade
Shipping Condition
Room Temperature
Target
BAC DNA, Plasmid DNA
System Type
PureLink™
Test Time
2 hrs
Yield
350 µg

Contents & Storage

• 200 ml Resuspension Buffer (R3)
• 1.5 ml RNase A
• 200 ml Lysis Buffer (L7)
• 200 ml Precipitation Buffer (N3)
• 2 × 250 ml Equilibration Buffer (EQ1)
• 2 × 500 ml Wash Buffer (W8)
• 250 ml Elution Buffer (E4)
• 30 ml TE Buffer
• 50 HiPure Columns
• 10 Column Holders

Store all components at room temperature.

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