|For Use With (Application)||Enzyme Immunoassay Detection|
|Shelf Life||24 Months|
|Description||Amplified IDEIA HP StAR™ Kit using Enzyme Immunoassay|
|Detectable Analytes||Helicobacter pylori antigen|
|Includes||Antibody coated microwells, sample diuent, positive Control, negative control, conjugate, wash buffer concentrate, substrate, stop solution.|
|Type||H. pylori Testing Kit|
|Catalog Number||Specifications||Unit Size||Price (USD)|
|K663011-2||Each||Request A Quote|
Helicobacter pylori infection is the most common cause of gastritis and is involved in gastric ulcer, duodenal ulcer, and gastric adenocarcinoma. It is also the causative agent in a high percentage of dyspepsia cases.
H. pylori bacteria have adapted to live in the acidic environment of the stomach. The enzyme urease cleaves urea into ammonia and carbon dioxide, thus neutralizing the acid and enabling H. pylori to survive the bactericidal conditions of the stomach. The production of catalase and superoxide dismutase protects the bacteria from neutrophilic attack in the stomach mucosa1.
Use Amplified IDEIA HP StAR for the initial diagnosis of H.pylori infections as well as for monitoring eradication success four to six weeks after completion of eradication therapy and also for the diagnosis of reinfection.
The Amplified IDEIA™ Hp StAR™ test is a sandwich-type enzyme immunoassay using immunoassay amplification technology for the determination of H. pylori antigens in feces.
The wells of the microplate are coated with monoclonal antibodies specific for H. pylori antigens. Supernatant of a fecal suspension, as well as horseradish peroxidase (HRP) labelled monoclonal antibodies (Antibody Conjugate), are added to the wells in one step. During incubation, H. pylori antigens present in the sample bind to the antibodies on the microplate and to the HRP labeled antibodies thus forming a ‘sandwich complex’. The wells are washed in order to remove unbound Antibody Conjugate. A colourless single-component enzyme substrate (Tetramethylbenzidine – TMB) is added. Bound HRP oxidizes TMB to a blue coloured product. By adding the Stop Solution the colour changes to yellow. The intensity of the colour is determined spectrophotometrically.
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