Cell Viability and Function Information

Learn about assays and techniques for detecting and monitoring cell health and function by flow cytometry, imaging, high-content analysis and microplate assays. Topics include apoptosis, autophagy, cell counting, proliferation and viability as well as cell cycle, endocytosis, ion indicators and ion channels, oxidative stress and more.

Cell viability and function features

BioProbes article

BioProbes article

Multiplex assays for robust cell health analyses

Here we feature an overview of plate-based viability assays which are a fundamental tool in drug discovery for evaluating the potency of compounds and the sensitivity of cell lines to specific agents.

Application note

Fluorescent viability assays on the Countess II FL Automated Cell Counter

Fluorescent viability assays on the Countess II FL Automated Cell Counter

Assessing cell viability is a key step in daily cell manipulation and is required for accurate and efficient downstream processing. In bright-field microscopy, the most commonly used method is trypan blue staining; however, many fluorescent options also exist and are commonly used in microscopy and flow cytometry applications.


Cell viability and function subtopics


Cell viability and function learning resources

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TypeTitleCategories
Application note (2011)Apoptosis detection: Apoptosis assays for the Attune Acoustic Focusing Cytometer Alexa Fluor, apoptosis, Attune/Attune NxT, cell structure-mitochondria, cell structure-plasma membrane, flow cytometer/flow cytometry, fluorescent dyes
Application note (2011)Bacterial analysis using the Attune Acoustic Focusing Cytometer Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, microbiology, viability
Application note (2011)Detection of human mesenchymal stem cells using the Attune Acoustic Focusing Cytometer Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, immunophenotyping, stem cell research
Application note (2012)Monitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screening ArrayScan, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, neuroscience, viability
Application note (2012)Microbiological applications using the Attune Acoustic Focusing Cytometer Attune/Attune NxT, cell health, flow cytometer/flow cytometry, fluorescent dyes, microbiology, viability
Application note (2013)Multiplexed mitosis and apoptosis analysis antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, particles
Application note (2014)High content cell cycle screening: Pairing FUCCI technology with Thermo Scientific HCS Studio 2.0 Software and FCS Express Image cytometry ArrayScan, cancer, cell cycle, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader
Application note (2014)Oxidative stress measurements made easy: CellROX reagents with simple workflow for high content imaging ArrayScan, CellInsight, CellROX, high content analysis, oxidative stress
Application note (2014)Elucidating the mechanism of cell death through the use of high content analysis apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Application note (2015)Guide to setting up hypoxic conditions on the EVOS FL Auto Imaging System with Onstage IncubatorEVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator, phagocytosis
Application note (2015)Quantitation of proliferating cells with the EVOS FL Auto Imaging Systemantibodies, antibody labeling, ArrayScan, cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, onstage incubator, phagocytosis
Application note (2015)Image tiling and stitching using the EVOS FL Auto Imaging Systemcell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, Imaging sample preparation, ReadyProbes
Application note (2015)Fluorescent viability assays on the Countess II FL Automated Cell Counterautomated cell counter, cell counting, Countess, EVOS, fluorescent dyes, ReadyProbes, viability
Application note (2015)Fluorescent apoptosis evaluation on the Countess II FL Automated Cell Counterapoptosis, automated cell counter, cell counting, Countess, EVOS, fluorescent dyes, viability
Application note (2015)Cell division and migration during wound healing visualized on the EVOS FL Auto Imaging Systemcell health, cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging
Application note (2015)Live/dead algal analysis with the Attune NxT Flow Cytometeralgae, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, viability
Application note (2015)No-wash, no-lyse detection of phagocytic cells via a pHrodo BioParticles functional assay in human whole blood on the Attune NxT Flow Cytometerantibodies, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immune system, immunophenotyping, particles, pH detection
Application note (2017)Flow cytometry analysis of dose response for apoptosis inductionapoptosis, Attune/Attune NxT, cancer, flow cytometer/flow cytometry
BioProbes articles (Issues 50–present day)BioProbes Journal of Cell Biology Applicationcell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification
BioProbes article (2017)Journal club—Measurement and characterization of apoptosis by flow cytometryflow cytometer/flow cytometry, apoptosis, fluorescent dyes
Molecular Probes HandbookMAXC Computer Program for calculating Free Ca2+ concentrations—Note 19.2ion channels, ion flux, ion indicators
Molecular Probes HandbookLoading and calibration of intracellular ion indicators—Note 19.1ion channels, ion flux, ion indicators
Molecular Probes HandbookCalcium regulation—Section 17.2ion channels, ion flux, ion indicators
Molecular Probes HandbookProbes for ion channels and carriers—Section 16.3ion channels, ion flux, ion indicators
Molecular Probes HandbookProbes useful at near-neutral pH—Section 20.2BCECF, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, pH detection, pH indicators, SNARF
Molecular Probes HandbookProbes useful at acidic pH—Section 20.3flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes HandbookpH indicator conjugates—Section 20.4flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes HandbookOverview of pH indicators—Section 20.1flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes HandbookNucleic acid stains—Section 8.1cell cycle, cell structure-nucleus, cell structure-plasma membrane, cell viability, DAPI, DNA binding dyes, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, membrane permeability, RNA binding dyes, SYTO, SYTOX
Molecular Probes HandbookFluorescent Na+ and K+ indicators—Section 21.1ion channels, ion flux, ion indicators
Molecular Probes HandbookDetecting chloride, phosphate, nitrite and other anions—Section 21.2ion channels, ion flux, ion indicators
Molecular Probes HandbookIntroduction to Ca2+ measurements with fluorescent indicators—Section 19.1ion channels, ion flux, ion indicators
Molecular Probes HandbookFluorescent Mg2+ indicators—Section 19.6ion channels, ion flux, ion indicators
Molecular Probes HandbookFluorescent indicators for Zn2+ and other metal ions—Section 19.7ion channels, ion flux, ion indicators
Molecular Probes HandbookFluorescent Ca2+ indicators excited with visible light—Section 19.3ion channels, ion flux, ion indicators
Molecular Probes HandbookFluorescent Ca2+ indicators excited with UV light—Section 19.2ion channels, ion flux, ion indicators
Molecular Probes HandbookFluorescent Ca2+ indicator conjugates—Section 19.4ion channels, ion flux, ion indicators
Molecular Probes HandbookChelators, calibration buffers, ionophores and cell-loading reagents—Section 19.8ion channels, ion flux, ion indicators
Molecular Probes HandbookProtein-based Ca2+ indicators—Section 19.5calcium flux, fluorescent dyes, ion indicators
Molecular Probes HandbookViability and cytotoxicity assay reagents—Section 15.2cell health, cell viability and function, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes HandbookViability and cytotoxicity assay kits for diverse cell types—Section 15.3cell health, cell viability and function, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes HandbookOverview of probes for cell viability, cell proliferation and live-cell function—Section 15.1cell health, cell death, cell proliferation, cell viability and function
Molecular Probes HandbookAssays for apoptosis and autophagy—Section 15.5apoptosis, autophagy, fluorescent dyes, fluorescence microscopy/fluorescence imaging, flow cytometer/flow cytometry
ProtocolVybrant MTT Cell Proliferation Assay Kitcell viability, microplate
ProtocolViability staining protocol for flow cytometrycell viability, flow cytometry
ProtocolSYTOX Dead Cell Stainscell viability, flow cytometry
ProtocolRed blood cell lysis protocols using eBioscience lysis bufferscell proliferation, flow cytometry
ProtocolReadyProbes Cell Viability Imaging Kit, Blue/Redcell viability, imaging
ProtocolReadyProbes Cell Viability Imaging Kit, Blue/Greencell viability, imaging
ProtocolPrestoBlue Cell Viability Reagent for microplatescell viability, microplate
ProtocolPrestoBlue and CyQUANT Direct Confirmation Assaycell viability, microplate
ProtocolPharmacological induction of apoptosis with camptothecincell vitality, flow cytometry, high content analysis, imaging, microplate
ProtocolNucRed Dead 647 ReadyProbes Reagent for viabilitycell viability, imaging
ProtocolNucGreen Dead 488 ReadyProbes Reagent for viabilitycell viability, imaging
ProtocolLIVE/DEAD Yeast Viability Kitcell viability, imaging
ProtocolLIVE/DEAD Violet Viability/Vitality Kitcell viability and cell vitality, flow cytometry
ProtocolLIVE/DEAD Viability/Cytotoxicity Kit for mammalian cellscell viability, imaging
ProtocolLIVE/DEAD Sperm Viability Kit for flow cytometrycell viability, flow cytometry
ProtocolLIVE/DEAD Sperm Viability Kit for imagingcell viability, imaging
ProtocolLIVE/DEAD Fixable Dead Cell Stainscell viability, flow cytometry
ProtocolLIVE/DEAD Cell Imaging Kit (488/570)cell viability, imaging
ProtocolLIVE/DEAD BacLight Bacterial Viability Kitcell viability, imaging
ProtocolLIVE BacLight Bacterial Gram Stain Kitcell viability, imaging
ProtocolImage-iT DEAD Green Kitcell viability, imaging
ProtocolHCS Mitochondrial Health Kitcell viability, high content analysis
ProtocolHCS LIVE/DEAD Green Kit using Hoechst 33342cell viability, high content analysis
ProtocolHCS LIVE/DEAD Green Kit using HCS NuclearMask Deep Redcell viability, high content analysis
ProtocolStaining cells with eFluor proliferation dyes for flow cytometrycell proliferation, flow cytometry
ProtocolCyQUANT Direct Microplate Reagent for cell viabilitycell viability, microplate
ProtocolClick-iT Plus EdU imaging kitscell proliferation, imaging
ProtocolClick-iT EdU imaging kitscell proliferation, imaging
ProtocolClick-iT EdU HCS assayscell proliferation, imaging
ProtocolCellTrace Violet Cell Proliferation Kitcell proliferation, flow cytometry
ProtocolCellTrace Far Red Cell Proliferation Kitcell proliferation, flow cytometry
ProtocolCellTrace CFSE Cell Proliferation Kitcell proliferation, flow cytometry
ProtocolCell preparation for flow cytometry protocolscell proliferation, flow cytometry
ProtocolBrdU staining protocol for flow cytometrycell proliferation, flow cytometry
ProtocolBrdU labeling and detectioncell proliferation, imaging
ProtocolAnnexin V staining protocol for flow cytometrycell vitality, flow cytometry
Scientific poster (2006)LipidTOX dyes for adipocyte staining in routine imaging applications cell differentiation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, stem cell research, viability
Scientific poster (2006)Cell cycle analysis using microplate cytometry: A comparison of laser and dye combinations Acumen Explorer, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader
Scientific poster (2006)New fluorescent reagents for the imaging-based assays for the analysis of drug-induced perturbations of cellular lipid metabolism ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, microplate reader, viability
Scientific poster (2006)Utility of 405 nm-excitable dyes in high content screening using an Acumen Explorer Microplate Cytometer Acumen Explorer, apoptosis, cell cycle, cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, violet laser-excited reagents
Scientific poster (2006)Novel violet-excited reagents for detection of viability and vitality  Alexa Fluor, cell health, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, viability, violet laser-excited reagents
Scientific poster (2007)Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry antibodies, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling
Scientific poster (2007)A new approach for the detection of intracellular glutathione by fluorescence microscopy and flow cytometry ArrayScan, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, live-cell imaging, microplate reader, viability, violet laser-excited reagents
Scientific poster (2007)Click chemistry-based detection of S-phase adherent cells using automated microscopy and image analysis Alexa Fluor, antibodies, ArrayScan, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader
Scientific poster (2007)Click catalyzed nucleic acid labeling as a novel replacement for BrdU antibody-based cell proliferation assay Alexa Fluor, antibodies, antibody labeling, cell cycle, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling
Scientific poster (2008)Living molecular probes for the next generation BacMam technology, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, ion flux/ion channels, ion indicators, live-cell imaging
Scientific poster (2008)Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Scientific poster (2008)Cell-based potassium ion channel screening with the FluxOR Assay BacMam technology, cell counting, Countess, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2008)Novel Click-IT TUNEL Assay for detection of cell death Alexa Fluor, antibodies, apoptosis, Click-iT, cytotoxicity, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, immunocytochemistry (ICC), viability
Scientific poster (2008)Effects of the thymidine analogues EdU and BrdU on cell viability and cycle progression Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, viability
Scientific poster (2008)Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry Alexa Fluor, antibodies, ArrayScan, cell cycle, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling, nucleic acid quantitation
Scientific poster (2008)Improving downstream results with automated cell counting automated cell counter, BacMam technology, cell counting, cell expansion, cell isolation, Countess, fluorescent dyes, hemocytometer, ion flux/ion channels, microplate reader
Scientific poster (2008)FluxOR, a universal potassium channel assay: Screening BacMam-delivered and stably expressed targets BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2008)Detection of DNA synthesis by automated microscopy and image analysis: Comparison of BRDU method and a new click chemistry-based EDU method Alexa Fluor, antibodies, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling
Scientific poster (2009)Cell-based assays for predictive hepatotoxicity measurements using high content imaging Alexa Fluor, ArrayScan, cell structure-plasma membrane, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, high content analysis, microplate reader, nucleic acid quantitation, viability
Scientific poster (2009)A non-radioactive method for detecting nascent protein synthesis by high-content imaging utilizing click chemistry Alexa Fluor, apoptosis, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, protein detection
Scientific poster (2009)Monitoring mitotic cells and DNA content by automated imaging and analysis Alexa Fluor, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability
Scientific poster (2009)Dual pulse labeling of S-phase population using click chemistry to measure changes in cell proliferation Alexa Fluor, cancer, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes
Scientific poster (2009)Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents
Scientific poster (2009)Dual pulse labeling using a new thymidine analog 5-ethynyl-2'-deoxyuridine (EdU) to detect alterations in S-phase progression by fluorescence microscopy and flow cytometry Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2009)High content imaging of endocytosis & phagocytosis using pHrodo conjugates Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability
Scientific poster (2009)Click chemistry-based detection of nascent RNA synthesis using high content imaging and fluorescence microscopy Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling, viability
Scientific poster (2009)A simple, fast and quantitative single-step dead-cell indicator for flow cytometry apoptosis, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, nucleic acid quantitation, viability
Scientific poster (2009)High content analysis of cytotoxicity by laser scanning fluorescence microplate cytometry Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, viability
Scientific poster (2009)Accuracy and precision comparison of the hemocytometer and automated cell counting methods automated cell counter, cell counting, Countess, hemocytometer, particles, viability
Scientific poster (2009)Dead cell stains in flow cytometry: A comprehensive analysis apoptosis, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, viability
Scientific poster (2009)High content imaging and analysis of mitotoxicity and cytotoxicity in fixed cells cell structure-mitochondria, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, viability
Scientific poster (2009)Quantitative analysis of genotoxicity and cytotoxicity to DNA damaging agents using high-content imaging Alexa Fluor, antibodies, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability
Scientific poster (2009)Cell-based potassium ion channel screening with BacMam using the FluxOR Assay BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2009)Click chemistry-based assays for fluorescence microscopy and high content imaging Alexa Fluor, apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid detection, protein detection, viability
Scientific poster (2010)Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry apoptosis, autophagy, caspase substrates, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress
Scientific poster (2010)Acoustic focusing cytometry: Sensitivity and throughput apoptosis, Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, phagocytosis
Scientific poster (2010)High content imaging of autophagy antibodies, ArrayScan, autophagy, BacMam technology, cell signaling, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader
Scientific poster (2010)Analysis of proliferation dynamics in primary and immortalized human cells Alexa Fluor, cancer, cell proliferation, CellTrace, circulatory system, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, fluorescent proteins, nucleic acid quantitation
Scientific poster (2010)Oxidative stress-induced protein modification: Application of clickable linoleic acid analogs Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, microplate reader, oxidative stress, protein detection, protein enrichment
Scientific poster (2010)Antibody- and fluorescent protein-based approaches to measuring autophagy in mammalian cells by fluorescence microscopy Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging
Scientific poster (2010)Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents
Scientific poster (2010)Novel tools to enable high resolution gene expression by investigating the nascent transcriptome Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, magnetic beads, nucleic acid labeling, PCR/qPCR, viability
Scientific poster (2010)Autophagosomal accumulation perturbs Golgi structure without affecting other organelles: Implications for autophagosome biogenesis Alexa Fluor, antibodies, autophagy, BacMam technology, cell structure-golgi, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging
Scientific poster (2010)A new generation of cellular imaging probes for neurosciences research autophagy, BacMam technology, CellLight, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels, ion indicators
Scientific poster (2010)Simultaneous analysis of cell death mechanisms and oxidative stress using live cell fluorescence microscopy apoptosis, ArrayScan, BacMam technology, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader, oxidative stress
Scientific poster (2010)A comparison of three techniques to induce efficient ex vivo T cell expansion Alexa Fluor, antibodies, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, immune system, magnetic beads, multicolor flow cytometry, viability
Scientific poster (2010)New fluorescent probes for live-cell imaging autophagy, cell cycle, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, pH detection, phagocytosis
Scientific poster (2010)Functional characterization of a novel fluorescent dye for proliferation analysis Alexa Fluor, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, violet laser-excited reagents
Scientific poster (2010)Non-cytotoxic near-IR DNA stain for cell cycle analysis in living cells cell cycle, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, live-cell imaging, nucleic acid labeling, viability
Scientific poster (2010)BacMam gene expression for functional and imaging applications BacMam technology, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels
Scientific poster (2010)Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry Alexa Fluor, cell cycle, cell proliferation, Click-iT, drug discovery, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2011)Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health ArrayScan, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress, viability
Scientific poster (2011)Simultaneous analysis of cell death mechanisms and oxidative stress using high content imaging Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress
Scientific poster (2011)Primary human cells from Life Technologies: Offerings, scale and applications in HCS and HTS formats Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, cell signaling, Click-iT, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Scientific poster (2011)Validation of high content imaging and analysis assays for cell health and cytotoxicity antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, cell health, cell proliferation, CellLight, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, pH detection
Scientific poster (2011)The next step in the analysis of lymphocyte proliferation Alexa Fluor, antibodies, Attune/Attune NxT, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, live-cell imaging, violet laser-excited reagents
Scientific poster (2011)The next generation of cell-based imaging assays for apoptosis, autophagy and oxidative stress from Molecular Probes Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress
Scientific poster (2011)Application of acoustic cytometry in microbiology Attune/Attune NxT, cell concentration, cell health, flow cytometer/flow cytometry, fluorescent dyes, microbiology, viability
Scientific poster (2012)Rapid, in vitro T lymphocyte cell tracing by acoustic focusing cytometry Attune/Attune NxT, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, magnetic beads, stem cell research
Scientific poster (2012)High sensitivity applications for the Applied Biosystems Attune Attune/Attune NxT, caspase substrates, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, viability
Scientific poster (2012)Cell-based analysis of oxidative stress, lipid peroxidation and lipid peroxidation-derived protein modifications using fluorescence microscopy flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis, lipid peroxidation, microplate reader, oxidative stress, viability
Scientific poster (2013)Pacific Green dye: A new fluorophore for violet laser excitation Alexa Fluor, antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot, viability, violet laser-excited reagents
Scientific poster (2013)Monitor caspase 3/7 activity without cell fixation: A novel apoptosis reagent from Molecular Probes Alexa Fluor, apoptosis, Attune/Attune NxT, caspase substrates, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry
Scientific poster (2013)Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health apoptosis, ArrayScan, caspase substrates, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress
Scientific poster (2013)Reactive oxygen probes—A broad range of colors with easier labeling: Novel CellROX reagents from Molecular Probes cells, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, oxidative stress, viability
Scientific poster (2013)Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping Alexa Fluor, Attune/Attune NxT, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping
Scientific poster (2013)Reactive oxygen probes—A broad range of colors with easier labeling and compatibility with fixation: Novel CellROX reagents from Molecular Probes cells, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, oxidative stress
Scientific poster (2013)Cells coming to life: Tools for visualizing immune response Attune/Attune NxT, endocytosis/phagocytosis, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunophenotyping, live-cell imaging, pH detection
Scientific poster (2013)Illuminating endocytosis with targeted pH-sensitive fluorescent compounds Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability
Scientific poster (2014)Copper-safe Click-iT Plus EdU proliferation assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detection Attune/Attune NxT, cell proliferation, Click-iT, flow cytometer/flow cytometry, multicolor flow cytometry
Scientific poster (2014)New fluorescent probes and sensors for visualizing endocytosis, lysosomal dynamics and autophagy autophagy, cell structure-lysosomes, endocytosis/phagocytosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader
Scientific poster (2014)Click-iT EdU Plus for flow cytometry: enhanced compatibility for multiplexing Attune/Attune NxT, cell proliferation, Click-iT, flow cytometer/flow cytometry, multicolor flow cytometry
Scientific poster (2015)A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, immunophenotyping, no lyse, phagocytosis, whole blood
Scientific poster (2015)Colorimetric dual labeled EdU/BrdU technology demonstrates contextual information and dynamics of proliferation in tissue apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
Scientific poster (2015)Visualizing the life and death of cells: novel probes and platforms apoptosis, ArrayScan, autophagy, cell death, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis
Scientific poster (2016)High content screening in MCF7 and MDA-MB231 cells show differential responses depending on oxygen levels and mechanistic readout for viability cell health, cell viability and function, drug discovery, high content analysis, high throughput screening
Scientific poster (2016)Cell mediated cytotoxicity in "untouched" whole blood Attune/Attune NxT, cell concentration, cell cycle, flow cytometer/flow cytometry, immunophenotyping, no lyse, whole blood
Scientific poster (2016)A novel non-cytotoxic fluorescent dye for cell proliferation analysis in flow cytometry Attune/Attune NxT, cell health, cell proliferation, CellTrace, EVOS, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging
Scientific poster (2016)Click colorimetric EdU proliferation and TUNEL: Click chemistry for brightfield microscopy apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
VideoCellEvent Caspase 3/7 reagent and apoptosis detection 
HeLa cells were loaded with 50 nM TMRM (red) followed by 5 μM CellEvent Caspase 3/7 Substrate (green). Cells were then treated with 0.5 μM staurosporine to induce apoptosis. TMRM detects the mitochondrial membrane potential and the signal disappears as the apoptosis onsets. CellEvent detects apoptosis, with green signal.
apoptosis, cell health, CellEvent, fluorescence microscopy/fluorescence imaging, live-cell imaging
VideoViability determination of HeLa cells using ReadyProbes Cell Viability Imaging Kit (Blue/Green) 
HeLa cells were loaded with NucBlue Live and NucGreen Dead (using 2 drops per ml) in complete media for 15 minutes at 37C. Stauroporine was then added to a final concentration of 1 µM and images were acquired every 30 min. for 18 hours using EVOS Auto Imaging System. All cells are stained with NucBlue Live, shown with blue nuclei. Over time an increase in the number of dead cells is observed as indicated by the appearance of green nuclei (NucGreen Dead).
cell structure-nucleus, cell viability, fluorescence microscopy/fluorescence imaging, live-cell imaging
VideoLIVE DEAD Cell Imaging kit on the EVOS Auto Imaging System 
Time course of cell death visualized using the LIVE/DEAD Cell Imaging kit (R37601). The LIVE/DEAD Cell Imaging kit is based on a cell-permeable dye (calcein, AM) that stains live, viable cells bright green and a cell-impermeable red marker that only stains dead and dying cells, which are characterized by compromised cell membranes. Labeled U-2 OS cells were treated 1 μM staurosporine and fluorescence images in the FITC and TexasRed channels were acquired every 5 minutes over 14 h on the EVOS Auto Imaging System using a 20x objective.
cell viability, fluorescence microscopy/fluorescence imaging, ion channels, ion flux, ion indicators, live-cell imaging
VideoCompilation of live cell imaging videos using Invitrogen fluorescent reagents 
This video demonstrates novel product brands from Invitrogen for live cell imaging include CellLight targeted fluorescent proteins, CellROX reagents for oxidative stress, CellEvent caspase 3/7 detection reagent, and many more fluorescent dyes and probes.
cell structure-mitochondria, CellEvent, CellLight, CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress
VideoClick-iT Plus TUNEL apoptosis assays 
The Click-iT Plus TUNEL assay detects apoptotic cells in tissue and cultured cell samples through the use of a small, highly specific labeling moiety and a bright fluorescent dye. After incorporation of the labeling moiety into DNA fragments, detection is achieved through a catalyzed "click" reaction using conditions mild enough to preserve the fluorescent signal from GFP or RFP.
apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging
VideoZ Stack image of HeLa cells labeled with CellLights reagents 
A series of images were captured on the EVOS FL Auto Cell Imaging System. Creating a Z-stack from these images allowed the observation of cellular cytoskeletal changes, which can be indicative of the loss of cell health. Methods HeLa cells grown in MatTek 6-well glass bottom culture plates were transduced with CellLights Tubulin-GFP and CellLight Mitochondria-RFP overnight at 37oC. The following day, NucBlue Live reagent (2 drops/mL) was added to the cultures. Cells were then imaged on an EVOS FL Auto Cell Imaging System with 100x oil immersion objective using the Z-stack function. The step size was set using the Nyquist formula and performed at 0.366 μm.
cell health, cell structure-all, CellLight, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging
VideoDetection of oxidative stress with CellROX Green in U2-OS cells 
U2-OS cells were plated at 200,000 cells per dish on a 35 mm glass bottom dish (MatTek) and cultured overnight. The cells were rinsed once and loaded with 5 µM CellROX Green Reagent (Cat. No. C10444) and 100 nM TMRM in Live Cell Imaging Solution (LCIS, Cat. No. A14291DJ) for 15 minutes at 37 degrees C. After loading, cells were imaged live with no washing every 20 seconds for seventy minutes on the DeltaVision Core inverted microscope at 37 degrees C. Menadione was added to a final concentration of 100 uM after ten minutes of baseline acquisition. The time lapse data shows the loss of signal from TMRM in the red channel as mitochondrial function decreases, concomitant with the onset of a nuclear signal in the green channel as CellRox Green is oxidized and the reagent migrates to the nucleus to generate a fluorogenic response as the active form of the dye binds to DNA.
CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress
VideoTime-lapse of cell migration and apoptosis during angiogenesis  
Angiogenesis pseudo-tube formation and apoptosis shown with time-lapse imaging. The time-lapse images were captured and assembled using an EVOS FL Auto Imaging System equipped with the EVOS Onstage Incubator.
angiogenesis, apoptosis, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator
VideoClick-iT Plus EdU Proliferation Assay 
Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies.
cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging
WebinarBasic techniques in autophagy research 
This webinar will introduce you to a series of analytical tools and techniques to help you identify and interrogate key features of autophagy. Topics to be covered include: Tips and tricks for selecting the right tools and achieving the best results Fluorescent proteins and antibodies used to analyze both live and fixed cells Analysis steps using a variety of multiplexing options, with quantitative methods for image analysis or fluorescence intensity measurement
autophagy, cell health, fluorescence microscopy/fluorescence imaging, fluorescent proteins, high content analysis, immunofluorescence (IF)
WebinarBasics of multicolorflow cytometry panel design 
With the proliferation of new fluorescent dyes, as well as instruments that can detect 18 or more parameters multicolor flow cytometry has become more popular and more accessible than ever. This webinar presented by Dr. Holden T. Maecker at Stanford University will discuss the caveats of good panel design, including: Rules for designing panels Examples and practical application of these rules Controls and standardization Relevance of panel design to new mass cytometry platforms
antibodies, compensation, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry, viability
WebinarThe meaning of life at the cellular level: detecting apoptosis with fluorescence 
In this webinar, Thermo Fisher Scientific scientists will demonstrate several solutions using fluorescent probes to study key components of the apoptotic machinery. The presentation includes: An overview of the features of apoptosis Key parameters that can be measured to assay apoptosis A comprehensive guide to available labeling and detection technologies for apoptosis research Tips and tricks to best implement those technologies
apoptosis, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent proteins
WebinarDNA content cell cycle analysis using flow cytometry 
Find out how careful acquisition and methodical preparation contribute to accurate and consistent DNA analysis. In this webinar we'll discuss: An overview of the methods and materials for using flow cytometry to determine cell cycle by measuring DNA content Selection of DNA dyes for live cell and fixed cell analysis Tips and tricks for consistent results
cell counting, cell cycle, cell proliferation, flow cytometer/flow cytometry
WebinarAn introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis 
In this free webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring Membrane structure, Mitochondrial function, Metabolism, Caspase activity, Membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes.
apoptosis, cell cycle, cell proliferation, flow cytometer/flow cytometry, multicolor flow cytometry, viability
WebinarThe meaning of life at the cellular level: Probing viability with fluorescence 
This webinar will provide an overview of features of healthy and unhealthy cells as well as describing key parameters that can be measured to assay cell viability. The webinar also offers a comprehensive guide to available labeling and detection technologies for cell health research as well as tips and tricks on how to best use them.
cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, viability
WebinarFlow cytometry in microbiological research 
In recent years the application of flow cytometry in microbiological research has expanded from detection and quantification of organisms to more complex studies including analysis of host-microbe interactions and detailed spatial and temporal analysis of microbial metabolism in different environments. During this webinar we will discuss how the multi-parametric nature of flow cytometry can be applied to microbiology and the advantages of using this application over traditional microbiological methods.
flow cytometer/flow cytometry, microbiology, multicolor flow cytometry, viability
WebinarAn introduction to flow cytometric analysis, Part 1: Cell proliferation analysis 
In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis.
apoptosis, cell cycle, cell proliferation, flow cytometer/flow cytometry, multicolor flow cytometry, viability
WebinarFrom the hood to the microscope; Revolutionizing cell-based imaging 
Join us for an interactive, educational webinar that showcases some of the latest advancements in cell-based research and imaging. Learn as we explore how to obtain superior results through the careful selection of reagents and the optimization of your imaging workflow— from growing cells on suitable surfaces through to image capture. Particular attention will be given to growing and monitoring cells for imaging, fluorescent labeling of live cells, critical considerations for time-lapse imaging and optimizing live cell imaging. Topics include: Choose a suitable imaging culture vessel for studying cell growth and viability using bright-field microscopy Label cells to maximize signal-to-noise for fluorescence imaging Prepare culture conditions and capture images without losing temporal data for time-lapse imaging Achieve hypoxic culture conditions by modifying gas conditions or employing cell spheroid cultures
cell health, EVOS, FLoid, fluorescence microscopy/fluorescence imaging, hypoxia, live-cell imaging, onstage incubator