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Western blot analysis of Bcl2 was performed by loading 2ul SeeBlue® Plus2 Prestained Protein Ladder (Product # LC5925) (lane 1) and 50ug HeLa (lane 2, HeLa (human)) cell lysate onto a 10% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 1% BSA/TBST for at least 1 hour at room temperature. Bcl2 was detected using a monoclonal antibody (Product #13-8800) at a concentration of 1ug/ml in blocking buffer overnight at 4°C on a rocking platform, followed by a goat anti-mouse IgG Alexa Fluor 680 secondary antibody (Product # A11357) at a dilution of 1:10,000 for at least 1 hour. Fluorescent detection was performed using the Odyssey® CLx imaging system (Li-cor Biosciences). Images generated by Joell Solan in Paul Lampe Lab at Fred Hutchinson Cancer Research Center.
|Tested species reactivity||Human|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Synthetic peptide corresponding to residues 41-54 of the human Bcl-2 protein. This sequence is not shared by the mouse Bcl-2 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Members of the Bcl-2 Family (including Bcl-2, Bcl-XLand Bax) play an important role in regulation of apoptosis. Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, Bax is considered to have a pro-apoptotic function. Regulation of apoptosis is supposed to involve both homo- and heterodimerization of different isoforms of Bax and Bcl-2. The Bax gene encodes different isoforms including Bax alpha (21 kDa) and Bax beta (24 kDa), whereas both isoforms contain the BH1, BH2 and BH3 domains, Bax beta has a unique carboxyl terminus and does not contain a hydrophobic transmembrane domain. Bcl-2 is also expressed in different Isoforms. Bcl-2 beta differs in the 3' UTR and coding region compared to variant alpha. Bcl-2 beta is shorter (22 kDa) and has a distinct C-terminus compared to Bcl-2 alpha (26 kDa).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Automated quantitative analysis of tissue microarray of 443 patients with colorectal adenocarcinoma: low expression of Bcl-2 predicts poor survival.
13-8800 was used in immunohistochemistry - paraffin section to investigate Bcl2 expression in colorectal adenocarcinoma
|Nicholson AD,Guo X,Sullivan CA,Cha CH||Journal of the American College of Surgeons (219:977)||2014|
Cytoprotective mechanisms in rats lung parenchyma with zinc deprivation.
13-8800 was used in immunohistochemistry to study the effect of zinc deficiency on airway epithelium
|Biaggio VS,Alvarez-Olmedo DG,Perez Chaca MV,Salvetti NR,Valdez SR,Fanelli MA,Ortega HH,Gomez NN,Gimenez MS||Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (27:305)||2014|
U1 Adaptor Oligonucleotides Targeting BCL2 and GRM1 Suppress Growth of Human Melanoma Xenografts In Vivo.
13-8800 was used in immunoprecipitation and western blot to present the first in vivo use of U Adaptors to target two different human genes implicated in melanomagenesis, B-cell lymphoma 2 and metabotropic glutamate receptor.
|Goraczniak R,Wall BA,Behlke MA,Lennox KA,Ho ES,Zaphiros NH,Jakubowski C,Patel NR,Zhao S,Magaway C,Subbie SA,Jenny Yu L,Lacava S,Reuhl KR,Chen S,Gunderson SI||Molecular therapy. Nucleic acids (2:null)||2013|
Enhanced heterodimerization of Bax by Bcl-2 mutants improves irradiated cell survival.
13-8800 was used in immunoprecipitation to compared the effects and mechanism of Bcl-2 and its functional variants on irradiated cell survival.
|Verma YK,Raghav PK,Raj HG,Tripathi RP,Gangenahalli GU||Apoptosis : an international journal on programmed cell death (18:212)||2013|
Idiopathic cervical fibrosis--a new member of IgG4-related sclerosing diseases: report of 4 cases, 1 complicated by composite lymphoma.
13-8800 was used in immunohistochemistry - paraffin section to report on four cases of idiopathic cervical fibrosis
|Cheuk W,Tam FK,Chan AN,Luk IS,Yuen AP,Chan WK,Hung TC,Chan JK||The American journal of surgical pathology (34:1678)||2010|
||Paracrine factors secreted by endothelial progenitor cells prevent oxidative stress-induced apoptosis of mature endothelial cells.||Yang Z,von Ballmoos MW,Faessler D,Voelzmann J,Ortmann J,Diehm N,Kalka-Moll W,Baumgartner I,Di Santo S,Kalka C||Atherosclerosis (211:103)||2010|
FKBP12-rapamycin-associated protein associates with mitochondria and senses osmotic stress via mitochondrial dysfunction.
13-8800 was used in western blot to study the relationship between FKBP12-rapamycin associated protein and mitochondrial dysfunction during osmatic stress.
|Desai BN,Myers BR,Schreiber SL||Proceedings of the National Academy of Sciences of the United States of America (99:4319)||2002|
|Human||Not Cited||Paracrine factors secreted by endothelial progenitor cells prevent oxidative stress-induced apoptosis of mature endothelial cells.||Yang Z,von Ballmoos MW,Faessler D,Voelzmann J,Ortmann J,Diehm N,Kalka-Moll W,Baumgartner I,Di Santo S,Kalka C||Atherosclerosis (211:103)||2010|
|Human||Not Cited||The chemopreventive agent phenethyl isothiocyanate sensitizes cells to Fas-mediated apoptosis.||Pullar JM,Thomson SJ,King MJ,Turnbull CI,Midwinter RG,Hampton MB||Carcinogenesis (25:765)||2004|
apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; PPP1R50; protein phosphatase 1 regulatory subunit 50; protein phosphatase 1, regulatory subunit 50
Bcl-2; BCL2; PPP1R50