Invitrogen

CD117 (c-Kit) Monoclonal Antibody (ACK2), PE-Cyanine7, eBioscience™

21 Published Figures

19 References

View all (216) c-Kit (CD117) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (21)

CD117 (c-Kit) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 22

CD117 (c-Kit) Antibody (25-1172-82) in Flow

Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.5 µg of Rat IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4031-82) (left) or 0.5 µg of Anti-Mouse CD117 (c-Kit) PE-Cyanine7 (right). Total viable cells were used for analysis.

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Product Details

25-1172-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 19 publications 19 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

ACK2

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_469646

Product Specific Information

Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.

Applications Reported: The ACK2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

ABIN1 (Q478) is Required to Prevent Hematopoietic Deficiencies through Regulating Type I IFNs Expression.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Wu X,Wang Y,Chen B,Liu Y,Li F,Ou Y,Zhang H,Wu X,Li X,Wang L,Rong W,Liu J,Xing M,Zhao X,Liu H,Ge L,Lv A,Wang L,Wang Z,Li M,Zhang H

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Mon Jan 01 00:00:00 UTC 2024

Engineered bone marrow as a clinically relevant ex vivo model for primary bone cancer research and drug screening.

Proceedings of the National Academy of Sciences of the United States of America

Griffin KH,Thorpe SW,Sebastian A,Hum NR,Coonan TP,Sagheb IS,Loots GG,Randall RL,Leach JK

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Tue Sep 26 00:00:00 UTC 2023

Landscape of mast cell populations across organs in mice and humans.

The Journal of experimental medicine

Tauber M,Basso L,Martin J,Bostan L,Pinto MM,Thierry GR,Houmadi R,Serhan N,Loste A,Blériot C,Kamphuis JBJ,Grujic M,Kjellén L,Pejler G,Paul C,Dong X,Galli SJ,Reber LL,Ginhoux F,Bajenoff M,Gentek R,Gaudenzio N

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Mon Oct 02 00:00:00 UTC 2023

The AML-associated K313 mutation enhances C/EBPα activity by leading to C/EBPα overexpression.

Cell death & disease

Gentle IE,Moelter I,Badr MT,Döhner K,Lübbert M,Häcker G

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Mon Jul 05 00:00:00 UTC 2021

The lifespan quantitative trait locus gene Securin controls hematopoietic progenitor cell function.

Haematologica

Brown A,Schuetz D,Han Y,Daria D,Nattamai KJ,Eiwen K,Sakk V,Pospiech J,Saller T,van Zant G,Wagner W,Geiger H

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Tue Apr 27 00:00:00 UTC 2021

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Thu Sep 17 00:00:00 UTC 2020

Loss of FGFR3 Delays Acute Myeloid Leukemogenesis by Programming Weakly Pathogenic CD117-Positive Leukemia Stem-Like Cells.

Frontiers in pharmacology

Guo C,Ran Q,Sun C,Zhou T,Yang X,Zhang J,Pang S,Xiao Y

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Tue Feb 16 00:00:00 UTC 2021

GATA4-dependent organ-specific endothelial differentiation controls liver development and embryonic hematopoiesis.

The Journal of clinical investigation

Géraud C,Koch PS,Zierow J,Klapproth K,Busch K,Olsavszky V,Leibing T,Demory A,Ulbrich F,Diett M,Singh S,Sticht C,Breitkopf-Heinlein K,Richter K,Karppinen SM,Pihlajaniemi T,Arnold B,Rodewald HR,Augustin HG,Schledzewski K,Goerdt S

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Wed Mar 01 00:00:00 UTC 2017

IL33-mediated ILC2 activation and neutrophil IL5 production in the lung response after severe trauma: A reverse translation study from a human cohort to a mouse trauma model.

PLoS medicine

Xu J,Guardado J,Hoffman R,Xu H,Namas R,Vodovotz Y,Xu L,Ramadan M,Brown J,Turnquist HR,Billiar TR

25-1172 was used in Flow cytometry/Cell sorting to suggest that IL33 may initiate early detrimental type 2 immune responses after trauma through ILC2 regulation of neutrophil IL5 production.

Sat Jul 01 00:00:00 UTC 2017

Low Testosterone Alters the Activity of Mouse Prostate Stem Cells.

The Prostate

Zhou Y,Copeland B,Otto-Duessel M,He M,Markel S,Synold TW,Jones JO

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Sat Apr 01 00:00:00 UTC 2017

A Functional Bioluminescent Zebrafish Screen for Enhancing Hematopoietic Cell Homing.

Stem cell reports

Astuti Y,Kramer AC,Blake AL,Blazar BR,Tolar J,Taisto ME,Lund TC

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Tue Jan 10 00:00:00 UTC 2017

Mutant U2AF1-expressing cells are sensitive to pharmacological modulation of the spliceosome.

Nature communications

Shirai CL,White BS,Tripathi M,Tapia R,Ley JN,Ndonwi M,Kim S,Shao J,Carver A,Saez B,Fulton RS,Fronick C,O'Laughlin M,Lagisetti C,Webb TR,Graubert TA,Walter MJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Mon Jan 09 00:00:00 UTC 2017

Core Circadian Clock Genes Regulate Leukemia Stem Cells in AML.

Cell

Puram RV,Kowalczyk MS,de Boer CG,Schneider RK,Miller PG,McConkey M,Tothova Z,Tejero H,Heckl D,Järås M,Chen MC,Li H,Tamayo A,Cowley GS,Rozenblatt-Rosen O,Al-Shahrour F,Regev A,Ebert BL

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Thu Apr 07 00:00:00 UTC 2016

Regulation of B-cell development and tolerance by different members of the miR-17∼92 family microRNAs.

Nature communications

Lai M,Gonzalez-Martin A,Cooper AB,Oda H,Jin HY,Shepherd J,He L,Zhu J,Nemazee D,Xiao C

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Tue Aug 02 00:00:00 UTC 2016

Different growth and metastatic phenotypes associated with a cell-intrinsic change of Met in metastatic melanoma.

Oncotarget

Adachi E,Sakai K,Nishiuchi T,Imamura R,Sato H,Matsumoto K

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Tue Oct 25 00:00:00 UTC 2016

Beta-Chemokine CCL15 Affects the Adhesion and Migration of Hematopoietic Progenitor Cells.

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie

Richter R,Rüster B,Bistrian R,Forssmann WG,Seifried E,Henschler R

25-1172 was used in Flow cytometry/Cell sorting to point to a regulation of HPCs by CCL15 and ultimately improve HPC short-term engraftment in stem cell transplantation.

Thu Jan 01 00:00:00 UTC 2015

TRPA1-dependent pruritus in IL-13-induced chronic atopic dermatitis.

Journal of immunology (Baltimore, Md. : 1950)

Oh MH,Oh SY,Lu J,Lou H,Myers AC,Zhu Z,Zheng T

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Sun Dec 01 00:00:00 UTC 2013

Mast cell-deficient Kit(W-sh) "Sash" mutant mice display aberrant myelopoiesis leading to the accumulation of splenocytes that act as myeloid-derived suppressor cells.

Journal of immunology (Baltimore, Md. : 1950)

Michel A,Schüler A,Friedrich P,Döner F,Bopp T,Radsak M,Hoffmann M,Relle M,Distler U,Kuharev J,Tenzer S,Feyerabend TB,Rodewald HR,Schild H,Schmitt E,Becker M,Stassen M

25-1172 was used in Flow cytometry/Cell sorting to demonstrate the broad effects that the Kit(W-sh) mutation has on key steps in myelopoiesis.

Sat Jun 01 00:00:00 UTC 2013

Genetic variation determines mast cell functions in experimental asthma.

Journal of immunology (Baltimore, Md. : 1950)

Becker M,Reuter S,Friedrich P,Doener F,Michel A,Bopp T,Klein M,Schmitt E,Schild H,Radsak MP,Echtenacher B,Taube C,Stassen M

25-1172 was used in Flow cytometry/Cell sorting to investigate the role of mast cells in allergic asthma.

Wed Jun 15 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: belly-spot; c-kit proto-oncogene protein; CD117; ckit; dominant spotting; Dominant white spotting; Mast/stem cell growth factor receptor Kit; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit

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Gene Aliases: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; Kit; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W

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UniProt ID: (Mouse) P05532

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Entrez Gene ID: (Mouse) 16590

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Function(s)

nucleotide binding protease binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity stem cell factor receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups cytokine binding protein homodimerization activity metal ion binding transmembrane signal receptor

Process(es)

Performance Guarantee

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CD117 (c-Kit) Monoclonal Antibody (ACK2), PE-Cyanine7, eBioscience™

CD117 (c-Kit) Antibody (25-1172-82) in Flow

Product Details

25-1172-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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