Invitrogen

CD117 (c-Kit) Monoclonal Antibody (ACK2), PE-Cyanine7, eBioscience™

Product Image Gallery

CD117 (c-Kit) Antibody in Flow Cytometry (Flow) — Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.5 µg of Rat IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4031-82) (left) or 0.5 µg of Anti-Mouse CD117 (c-Kit) PE-Cyanine7 (right). Total viable cells were used for analysis.

Product Details

25-1172-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	1 µg/test	View 13 publications 13 publications

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG2b, kappa
Recommended Isotype Control	Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	ACK2
Conjugate	PE-Cyanine7 Alexa Fluor 700 APC APC-eFluor 780 Functional Grade PE PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 702 Unconjugated
Excitation/Emission Max	569/780 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_469646

Product Specific Information

Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.

Applications Reported: The ACK2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

c-Kit, also known as mast/stem cell growth factor receptor (SCFR) or CD117, is a trans membrane type III tyrosine kinase receptor encoded by the c-Kit proto-oncogene, located on chromosome 4 in humans. It is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

The AML-associated K313 mutation enhances C/EBPα activity by leading to C/EBPα overexpression.

Cell death & disease

Gentle IE,Moelter I,Badr MT,Döhner K,Lübbert M,Häcker G

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

IL33-mediated ILC2 activation and neutrophil IL5 production in the lung response after severe trauma: A reverse translation study from a human cohort to a mouse trauma model.

PLoS medicine

Xu J,Guardado J,Hoffman R,Xu H,Namas R,Vodovotz Y,Xu L,Ramadan M,Brown J,Turnquist HR,Billiar TR

25-1172 was used in Flow cytometry/Cell sorting to suggest that IL33 may initiate early detrimental type 2 immune responses after trauma through ILC2 regulation of neutrophil IL5 production.

Sat Jul 01 00:00:00 EDT 2017

Low Testosterone Alters the Activity of Mouse Prostate Stem Cells.

The Prostate

Zhou Y,Copeland B,Otto-Duessel M,He M,Markel S,Synold TW,Jones JO

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

GATA4-dependent organ-specific endothelial differentiation controls liver development and embryonic hematopoiesis.

The Journal of clinical investigation

Géraud C,Koch PS,Zierow J,Klapproth K,Busch K,Olsavszky V,Leibing T,Demory A,Ulbrich F,Diett M,Singh S,Sticht C,Breitkopf-Heinlein K,Richter K,Karppinen SM,Pihlajaniemi T,Arnold B,Rodewald HR,Augustin HG,Schledzewski K,Goerdt S

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

A Functional Bioluminescent Zebrafish Screen for Enhancing Hematopoietic Cell Homing.

Stem cell reports

Astuti Y,Kramer AC,Blake AL,Blazar BR,Tolar J,Taisto ME,Lund TC

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Tue Jan 10 00:00:00 EST 2017

Mutant U2AF1-expressing cells are sensitive to pharmacological modulation of the spliceosome.

Nature communications

Shirai CL,White BS,Tripathi M,Tapia R,Ley JN,Ndonwi M,Kim S,Shao J,Carver A,Saez B,Fulton RS,Fronick C,O'Laughlin M,Lagisetti C,Webb TR,Graubert TA,Walter MJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Mon Jan 09 00:00:00 EST 2017

Regulation of B-cell development and tolerance by different members of the miR-17∼92 family microRNAs.

Nature communications

Lai M,Gonzalez-Martin A,Cooper AB,Oda H,Jin HY,Shepherd J,He L,Zhu J,Nemazee D,Xiao C

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Tue Aug 02 00:00:00 EDT 2016

Core Circadian Clock Genes Regulate Leukemia Stem Cells in AML.

Cell

Puram RV,Kowalczyk MS,de Boer CG,Schneider RK,Miller PG,McConkey M,Tothova Z,Tejero H,Heckl D,Järås M,Chen MC,Li H,Tamayo A,Cowley GS,Rozenblatt-Rosen O,Al-Shahrour F,Regev A,Ebert BL

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Thu Apr 07 00:00:00 EDT 2016

Beta-Chemokine CCL15 Affects the Adhesion and Migration of Hematopoietic Progenitor Cells.

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie

Richter R,Rüster B,Bistrian R,Forssmann WG,Seifried E,Henschler R

25-1172 was used in Flow cytometry/Cell sorting to point to a regulation of HPCs by CCL15 and ultimately improve HPC short-term engraftment in stem cell transplantation.

Thu Jan 01 00:00:00 EST 2015

TRPA1-dependent pruritus in IL-13-induced chronic atopic dermatitis.

Journal of immunology (Baltimore, Md. : 1950)

Oh MH,Oh SY,Lu J,Lou H,Myers AC,Zhu Z,Zheng T

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 25-1172-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2013

Mast cell-deficient Kit(W-sh) "Sash" mutant mice display aberrant myelopoiesis leading to the accumulation of splenocytes that act as myeloid-derived suppressor cells.

Journal of immunology (Baltimore, Md. : 1950)

Michel A,Schüler A,Friedrich P,Döner F,Bopp T,Radsak M,Hoffmann M,Relle M,Distler U,Kuharev J,Tenzer S,Feyerabend TB,Rodewald HR,Schild H,Schmitt E,Becker M,Stassen M

25-1172 was used in Flow cytometry/Cell sorting to demonstrate the broad effects that the Kit(W-sh) mutation has on key steps in myelopoiesis.

Sat Jun 01 00:00:00 EDT 2013

Genetic variation determines mast cell functions in experimental asthma.

Journal of immunology (Baltimore, Md. : 1950)

Becker M,Reuter S,Friedrich P,Doener F,Michel A,Bopp T,Klein M,Schmitt E,Schild H,Radsak MP,Echtenacher B,Taube C,Stassen M

25-1172 was used in Flow cytometry/Cell sorting to investigate the role of mast cells in allergic asthma.

Wed Jun 15 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: belly-spot; c-kit proto-oncogene protein; CD117; ckit; dominant spotting; Dominant white spotting; Mast/stem cell growth factor receptor Kit; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit

Gene Aliases: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; Kit; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W

UniProt ID: (Mouse) P05532

Entrez Gene ID: (Mouse) 16590

Molecular Function: transmembrane signal receptor

Function(s)

Process(es)

nucleotide binding protease binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity stem cell factor receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups cytokine binding protein homodimerization activity metal ion binding

CD117 (c-Kit) Monoclonal Antibody (ACK2), PE-Cyanine7, eBioscience™

Product Details

25-1172-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

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