Invitrogen

CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), eBioscience™

Advanced Verification

This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.

37 Published Figures

44 References

View all (128) E-cadherin antibodies

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Datasheet

Protocols

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CD324 (E-Cadherin) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of E-cadherin was performed using 90% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1) (Product # 14-3249-82) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rat IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11006) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing plasma membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CD324 (E-Cadherin) Antibody in Western Blot (WB) — Immunoblot analysis of reduced MDCK cell line lysates with 2 µg/mL of Anti-Human CD324 (E-Cadherin) Purified and revealed with Anti-Rat IgG HRP. Lane 1: EDTA treated cells and Lane 2: Trypsin treated cells to remove from tissue culture dish.

CD324 (E-Cadherin) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Figure 4 Fluorescence staining of epithelial markers in the lungs. Notes: Lung sections from SCID-beige mice administered intravenously with PKH26-labeled hADSCs were observed under aconfocal laser microscope. ( A ) Fluorescence of PKH-26 (red) was observed with nuclear counterstaining by DAPI (blue) (x200). ( B ) Expression of epithelial cells markers TTF-1, Pro SP-C, beta-Catenin, and CDH1 visualized by Alexa Fluor 488 (green) in PKH26-labeled hADSCs (red) (x400). Arrow shows Alexa Flour 488 and PKH-26 merged cells. Scale bar shows 50 mum. Data are representative of more than three independent experiments. Abbreviations: hADSCs, human adipose-derived mesenchymal stem cells; DAPI, 4',6-diamidino-2-phenylindole; TTF-1, thyroid transcription factor-1; SP-C, surfactant protein C; CDH1, cadherin-1.

CD324 (E-Cadherin) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — Figure 3 Late Reprogramming Markers Are Enriched in the N+E+ Population (A) Flow cytometry plot of reprogramming culture stained with Nanog-647 and E-cadherin-488. (B) RNA-Seq read counts (transcripts per million [TPM]) for E-cadherin, labeled Cdh1 in figure, (left, Y axis) and Nanog (right, Y axis) for N+E+ and N+E- populations from three independent experiments. (C) Heatmap of genes 1.5-fold differentially expressed between N+E+ and N+E- populations (set to 1) ranked by average TPM from three independent experiments. (D) Functional annotation of gene expression in N+E+ and N+E- populations. (E) Left: Immunofluorescence images of NANOG, E-cadherin, and DPPA4 on d11 of NSC reprogramming. Right: Counts from two independent experiments of Dppa4+ colonies in N+E+ and N+E- colonies are stacked. Scale bar, 50 mum. Insets, magnification of field. (F) Comparison of d14 N+ and N+E+ colonies following withdrawal of dox on the day indicated on the X axis. Counts from four independent experiments are stacked. No significant difference between N+ and N+E+ colonies by paired two-tailed t test.

CD324 (E-Cadherin) Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunohistochemistry (Frozen)

CD324 (E-Cadherin) Antibody in Immunohistochemistry (IHC) — Fig. 4 Caspase-11 mediates STAT1 activation during CAC. a Western blot analysis of phosho-/total STAT1, STAT3, IkappaBalpha, and actin (loading control) in colon homogenates from AOM/DSS-treated WT and Casp11 -/- mice on day 105 post initial AOM IP injection. Each lane represents an individual mouse. b Total STAT1 and c pSTAT1 expression densitometry (relative to actin) in colon homogenates from AOM/DSS-treated WT and Casp11 -/- mice. ** p < 0.01 (two-tailed unpaired t-test). Representative immunofluorescent images of d pSTAT1; and e total STAT1; co-stained for the epithelial marker E-cadherin (red) and DAPI (blue) in distal colon sections from AOM/DSS-treated WT and Casp11 -/- mice at day 105 (scale bar = 20 mum)

CD324 (E-Cadherin) Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunohistochemistry (PFA fixed)

CD324 (E-Cadherin) Antibody in Flow Cytometry (Flow) — Figure 1 Epithelial-to-Mesenchymal Transition Reported by Historical Lineage-Tracing Reporter and Current E-Cadherin Status in Murine Metastatic Mammary Carcinoma Model (A) Schematic of fluorescent transgenic mouse model, carrying polyoma middle-T (PyMT) oncogene driven by the mammary gland specific MMTV promoter, Cre recombinase under the control of the fsp1 promoter, endogenous E-cad labeled with mCFP, and ubiquitous expression from the R26 locus of membranous RFP-STOP, which is flanked by lox P sites, leading to excision and subsequent expression of membranous GFP upon Cre expression. (B) Top panel: representative multi-photon images of the fluorescent PyMT mammary tumors from recipient mice. Scale bar, 100 mum. Bottom panel: high-magnification images of the E-cad-mCFP and RFP channel of the boxed area in the top left image. Scale bar, 50 mum. (C) Representative scatterplot from flow cytometry analysis of primary tumor of recipient mice for either absence of endogenous E-cad-mCFP expression and antibody staining (E-cad LO ) or presence of high expression of E-cad and staining for Ab (E-cad HI tumor cells) or subdivision of RFP + or GFP + cells. (D) Relative mRNA expression of classical EMT marker genes (E-cad, Vimentin [Vim], fibronectin [FN], Zeb1, N-cad [N-cadherin], Slug) determined using qPCR of sorted RFP + E-cad Hi , RFP + E-cad LO , RFP + GFP + , and GFP + cells from primary tumors. Plotted mean and SD of n = 3 mice. (E) Quantification of lung metastases of all tran

CD324 (E-Cadherin) Antibody in Neutralization (Neu) — Figure 3 rLRR20 and Leptospira infection downregulated E-cadherin expression. Neutralizing anti-E-cadherin antibody (Neu-alpha-E-cad) and short interfering RNA against E-cadherin (si-E-cad) were used to verify the regulatory effects of rLRR20 on E-cadherin expression in HK2s and hRPTECs. Effect of alpha-E-cad, which inhibits the interaction between rLRR20 and E-cadherin, on the rLRR20-mediated degradation of E-cadherin in HK2s ( A ) and hRPTECs ( B ). Effect of si-E-cad on the rLRR20-mediated degradation of E-cadherin in HK2s ( C ) and hRPTECs ( D ). Effect of pathogenic ( L. santarosai ) and non-pathogenic ( L. biflexa ) Leptospira species on the E-cadherin levels in HK2s ( E ) and hRPTECs ( F ). beta-actin was used as the internal control. EMJH, Ellinghausen-McCullough-Johnson-Harris medium. *** p < 0.001; ** p < 0.01; * p < 0.05.

CD324 (E-Cadherin) Antibody — Antibody specificity was demonstrated by showing that antibodies raised against the same target protein perform similarly. Immunofluorescence of E-cadherin using CD324 (E-Cadherin) Monoclonal Antibody (Product # 14-3249-82) performed along with another E-cadherin antibody (Product # MA5-14458) shows similar localization pattern in MCF7 cells. {IAV}

Product Details

14-3249-82

Applications	Tested Dilution	Publications
Western Blot (WB)	2 µg/mL	View 4 publications 4 publications
Immunohistochemistry (IHC)	-	View 13 publications 13 publications
Immunohistochemistry (PFA fixed) (IHC (PFA))	-	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	Assay-Dependent	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	5 µg/mL	View 13 publications 13 publications
Flow Cytometry (Flow)	Assay-Dependent	View 10 publications 10 publications
ELISA (ELISA)	Assay-Dependent	-
Immunoprecipitation (IP)	Assay-Dependent	-
Neutralization (Neu)	-	View 1 publication 1 publication
Functional Assay (FN)	Assay-Dependent	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Dog, Human, Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	DECMA-1
Conjugate	Unconjugated Alexa Fluor 488 Biotin eFluor 660 Functional Grade PerCP-eFluor 710
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_1210458

Product Specific Information

Description: The monoclonal antibody DECMA-1 recognizes mouse, human and canine CD324 also known as E-cadherin (Epithelial cadherin) or uvomorulin. Like the other cadherin family members P and N cadherin, E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion. These proteins share a common basic structure. The extracellular portions of the proteins are largely composed of repeating domains, each with two consensus Ca2+-binding motifs. The cytoplasmic domain interacts with a-, b-, and g-catenins and actinins. These catenins connect E-cadherin with the cytoskeleton.

Expression is found in most epidermal cells including melanocytes and kerotinocytes. E-cadherin is localized at the intercellular boundaries of epithelial cells in several tissues, and is thought to play a role in maintenance of tissue integrity. Loss of E-cadherin function has been implicated in the progression of a variety of cancers.

E-Cadherin protein is sensitive to trypsin treatment, so exposure to trypsin should be minimized or avoided.

The monoclonal antibody DECMA-1 has been shown to have functional activity by disrupting adhesion in human, mouse and dog cells.

Applications Reported: This DECMA-1 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, western blotting, immunohistochemical staining of frozen tissue sections, and ELISA. (Please use Functional Grade purified DECMA-1, cat. 16-3249, in functional assays.).

Applications Tested: This DECMA-1 antibody has been tested by western blot analysis of EDTA treated MDCK cell line. This can be used at less than or equal to 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway.

Molecular medicine reports

Li L,Hu Y,Chen D,Zhu J,Bao W,Xu X,Chen H,Chen W,Feng R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunocytochemistry

Sat Jan 01 00:00:00 EST 2022

Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation.

Journal of oncology

He Z,Lei S,Liang F,Tan L,Zhang W,Xie L,Zheng H,Lu Y

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Western Blot

Fri Dec 31 00:00:00 EST 2021

Crosstalk between E-Cadherin/β-Catenin and NF-κB Signaling Pathways: The Regulation of Host-Pathogen Interaction during Leptospirosis.

International journal of molecular sciences

Hsu SH,Chou LF,Hong CH,Chang MY,Tsai CY,Tian YC,Yang HY,Yang CW

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Neutralization

Sat Dec 04 00:00:00 EST 2021

EGFR Transgene Stimulates Spontaneous Formation of MCF7 Breast Cancer Cells Spheroids with Partly Loss of HER3 Receptor.

International journal of molecular sciences

Troitskaya O,Novak D,Nushtaeva A,Savinkova M,Varlamov M,Ermakov M,Richter V,Koval O

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunocytochemistry

Mon Nov 29 00:00:00 EST 2021

Human Adipose-Derived Mesenchymal Stem Cells Ameliorate Elastase-Induced Emphysema in Mice by Mesenchymal-Epithelial Transition.

International journal of chronic obstructive pulmonary disease

Fujioka N,Kitabatake M,Ouji-Sageshima N,Ibaraki T,Kumamoto M,Fujita Y,Hontsu S,Yamauchi M,Yoshikawa M,Muro S,Ito T

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunocytochemistry

Mon Nov 08 00:00:00 EST 2021

Human Adipose-Derived Mesenchymal Stem Cells Ameliorate Elastase-Induced Emphysema in Mice by Mesenchymal-Epithelial Transition.

International journal of chronic obstructive pulmonary disease

Fujioka N,Kitabatake M,Ouji-Sageshima N,Ibaraki T,Kumamoto M,Fujita Y,Hontsu S,Yamauchi M,Yoshikawa M,Muro S,Ito T

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunocytochemistry

Mon Nov 08 00:00:00 EST 2021

Efficient Messenger RNA Delivery to the Kidney Using Renal Pelvis Injection in Mice.

Pharmaceutics

Oyama N,Kawaguchi M,Itaka K,Kawakami S

14-3249-82 was used in Immunohistochemistry to conclude that the safety and efficacy of mRNA-loaded nanomicelles would make distinct contributions to the development of mRNA therapeutics for the kidney.

Fri Oct 29 00:00:00 EDT 2021

Opposing effects of Wnt/β-catenin signaling on epithelial and mesenchymal cell fate in the developing cochlea.

Development (Cambridge, England)

Billings SE,Myers NM,Quiruz L,Cheng AG

14-3249-82 was used in Immunohistochemistry to show that Wnt activation drives transition between epithelial and mesenchymal states in a cell type-dependent manner.

Tue Jun 01 00:00:00 EDT 2021

MicroRNA-384 inhibits nasopharyngeal carcinoma growth and metastasis via binding to Smad5 and suppressing the Wnt/β-catenin axis.

Cytotechnology

Zeng X,Liao H,Wang F

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Western Blot

Thu Apr 01 00:00:00 EDT 2021

Leptin Modulates the Metastasis of Canine Inflammatory Mammary Adenocarcinoma Cells Through Downregulation of Lysosomal Protective Protein Cathepsin A (CTSA).

International journal of molecular sciences

Kim JW,Mahiddine FY,Kim GA

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Western Blot

Wed Nov 25 00:00:00 EST 2020

Shigella flexneri Disruption of Cellular Tension Promotes Intercellular Spread.

Cell reports

Duncan-Lowey JK,Wiscovitch AL,Wood TE,Goldberg MB,Russo BC

14-3249 was used in Western Blotting to investigate the formation of plasma membrane protrusions and intercellular spread bin Shigella flexneri.

Tue Nov 24 00:00:00 EST 2020

Genome-wide CRISPR screen identifies noncanonical NF-κB signaling as a regulator of density-dependent proliferation.

eLife

Fomicheva M,Macara IG

14-3249 was used in Immunohistochemistry-immunofluorescence to discover that non-canonical NF-κB signalling is a regulator of density-dependent proliferation, which operates independently of known density-dependent proliferation mechanisms.

Fri Nov 13 00:00:00 EST 2020

Mechanisms of stretch-mediated skin expansion at single-cell resolution.

Nature

Aragona M,Sifrim A,Malfait M,Song Y,Van Herck J,Dekoninck S,Gargouri S,Lapouge G,Swedlund B,Dubois C,Baatsen P,Vints K,Han S,Tissir F,Voet T,Simons BD,Blanpain C

14-3249 was used in Immunohistochemistry to define the step-by-step mechanisms that control stretch-mediated tissue expansion at single-cell resolution in vivo.

Sat Aug 01 00:00:00 EDT 2020

Heterotypic cell-cell communication regulates glandular stem cell multipotency.

Nature

Centonze A,Lin S,Tika E,Sifrim A,Fioramonti M,Malfait M,Song Y,Wuidart A,Van Herck J,Dannau A,Bouvencourt G,Dubois C,Dedoncker N,Sahay A,de Maertelaer V,Siebel CW,Van Keymeulen A,Voet T,Blanpain C

14-3249 was used in Immunohistochemistry-immunofluorescence to demonstrate that heterotypic communication between LCs and BCs is essential to maintain lineage fidelity in glandular epithelial stem cells.

Sat Aug 01 00:00:00 EDT 2020

GDNF drives rapid tubule morphogenesis in a novel 3D in vitro model for ADPKD.

Journal of cell science

Dixon EE,Maxim DS,Halperin Kuhns VL,Lane-Harris AC,Outeda P,Ewald AJ,Watnick TJ,Welling PA,Woodward OM

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunocytochemistry

Thu Jul 16 00:00:00 EDT 2020

Epithelial Vegfa Specifies a Distinct Endothelial Population in the Mouse Lung.

Developmental cell

Vila Ellis L,Cain MP,Hutchison V,Flodby P,Crandall ED,Borok Z,Zhou B,Ostrin EJ,Wythe JD,Chen J

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunocytochemistry

Mon Mar 09 00:00:00 EDT 2020

Fsp1-Mediated Lineage Tracing Fails to Detect the Majority of Disseminating Cells Undergoing EMT.

Cell reports

Bornes L,van Scheppingen RH,Beerling E,Schelfhorst T,Ellenbroek SIJ,Seinstra D,van Rheenen J

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Flow Cytometry

Tue Nov 26 00:00:00 EST 2019

Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity.

The EMBO journal

Tran KA,Dillingham CM,Sridharan R

14-3249 was used in Immunocytochemistry-immunoflourescence to investigate the link between the removal of H3K9 methylation and DNA methylation during reprogramming of somatic cells pluripotency.

Fri Nov 15 00:00:00 EST 2019

Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2.

Nature communications

Malik V,Glaser LV,Zimmer D,Velychko S,Weng M,Holzner M,Arend M,Chen Y,Srivastava Y,Veerapandian V,Shah Z,Esteban MA,Wang H,Chen J,Schöler HR,Hutchins AP,Meijsing SH,Pott S,Jauch R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Flow Cytometry

Fri Aug 02 00:00:00 EDT 2019

Defining Reprogramming Checkpoints from Single-Cell Analyses of Induced Pluripotency.

Cell reports

Tran KA,Pietrzak SJ,Zaidan NZ,Siahpirani AF,McCalla SG,Zhou AS,Iyer G,Roy S,Sridharan R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunocytochemistry

Tue May 07 00:00:00 EDT 2019

Defining Reprogramming Checkpoints from Single-Cell Analyses of Induced Pluripotency.

Cell reports

Tran KA,Pietrzak SJ,Zaidan NZ,Siahpirani AF,McCalla SG,Zhou AS,Iyer G,Roy S,Sridharan R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunocytochemistry

Tue May 07 00:00:00 EDT 2019

Caspase-11 regulates the tumour suppressor function of STAT1 in a murine model of colitis-associated carcinogenesis.

Oncogene

Flood B,Manils J,Nulty C,Flis E,Kenealy S,Barber G,Fay J,Mills KHG,Kay EW,Creagh EM

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunohistochemistry

Mon Apr 01 00:00:00 EDT 2019

Early lineage segregation of multipotent embryonic mammary gland progenitors.

Nature cell biology

Wuidart A,Sifrim A,Fioramonti M,Matsumura S,Brisebarre A,Brown D,Centonze A,Dannau A,Dubois C,Van Keymeulen A,Voet T,Blanpain C

14-3249 was used in Immunocytochemistry-immunoflourescence to study the timing and mechanisms mediating early lineage segregation of multipotent progenitors during mammary gland development.

Fri Jun 01 00:00:00 EDT 2018

β-Catenin maintains lung epithelial progenitors after lung specification.

Development (Cambridge, England)

Ostrin EJ,Little DR,Gerner-Mauro KN,Sumner EA,Ríos-Corzo R,Ambrosio E,Holt SE,Forcioli-Conti N,Akiyama H,Hanash SM,Kimura S,Huang SXL,Chen J

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunohistochemistry

Fri Mar 09 00:00:00 EST 2018

Differential requirements of androgen receptor in luminal progenitors during prostate regeneration and tumor initiation.

eLife

Chua CW,Epsi NJ,Leung EY,Xuan S,Lei M,Li BI,Bergren SK,Hibshoosh H,Mitrofanova A,Shen MM

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunofluorescence

Mon Jan 15 00:00:00 EST 2018

Differential requirements of androgen receptor in luminal progenitors during prostate regeneration and tumor initiation.

eLife

Chua CW,Epsi NJ,Leung EY,Xuan S,Lei M,Li BI,Bergren SK,Hibshoosh H,Mitrofanova A,Shen MM

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunofluorescence

Mon Jan 15 00:00:00 EST 2018

Constitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming.

Cell stem cell

Ruetz T,Pfisterer U,Di Stefano B,Ashmore J,Beniazza M,Tian TV,Kaemena DF,Tosti L,Tan W,Manning JR,Chantzoura E,Ottosson DR,Collombet S,Johnsson A,Cohen E,Yusa K,Linnarsson S,Graf T,Parmar M,Kaji K

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Flow Cytometry

Thu Dec 07 00:00:00 EST 2017

A Unifying Theory of Branching Morphogenesis.

Cell

Hannezo E,Scheele CLGJ,Moad M,Drogo N,Heer R,Sampogna RV,van Rheenen J,Simons BD

14-3249 was used in Immunohistochemistry-immunofluorescence to elucidate how complex branched epithelial structures develop as a self-organised process, without recourse to a rigid deterministic sequence of genetically programmed events.

Thu Sep 21 00:00:00 EDT 2017

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells.

Journal of visualized experiments : JoVE

Lyons-Cohen MR,Thomas SY,Cook DN,Nakano H

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunofluorescence

Wed Apr 05 00:00:00 EDT 2017

Identity and dynamics of mammary stem cells during branching morphogenesis.

Nature

Scheele CL,Hannezo E,Muraro MJ,Zomer A,Langedijk NS,van Oudenaarden A,Simons BD,van Rheenen J

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunocytochemistry

Thu Feb 16 00:00:00 EST 2017

Identity and dynamics of mammary stem cells during branching morphogenesis.

Nature

Scheele CL,Hannezo E,Muraro MJ,Zomer A,Langedijk NS,van Oudenaarden A,Simons BD,van Rheenen J

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunocytochemistry

Thu Feb 16 00:00:00 EST 2017

Tumour stroma-derived lipocalin-2 promotes breast cancer metastasis.

The Journal of pathology

Ören B,Urosevic J,Mertens C,Mora J,Guiu M,Gomis RR,Weigert A,Schmid T,Grein S,Brüne B,Jung M

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

Quantitative lineage tracing strategies to resolve multipotency in tissue-specific stem cells.

Genes & development

Wuidart A,Ousset M,Rulands S,Simons BD,Van Keymeulen A,Blanpain C

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunofluorescence

Wed Jun 01 00:00:00 EDT 2016

Alternative Routes to Induced Pluripotent Stem Cells Revealed by Reprogramming of the Neural Lineage.

Stem cell reports

Jackson SA,Olufs ZP,Tran KA,Zaidan NZ,Sridharan R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Flow Cytometry

Tue Mar 08 00:00:00 EST 2016

Alternative Routes to Induced Pluripotent Stem Cells Revealed by Reprogramming of the Neural Lineage.

Stem cell reports

Jackson SA,Olufs ZP,Tran KA,Zaidan NZ,Sridharan R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Flow Cytometry

Tue Mar 08 00:00:00 EST 2016

Epithelial-to-mesenchymal transition is dispensable for metastasis but induces chemoresistance in pancreatic cancer.

Nature

Zheng X,Carstens JL,Kim J,Scheible M,Kaye J,Sugimoto H,Wu CC,LeBleu VS,Kalluri R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunohistochemistry

Thu Nov 26 00:00:00 EST 2015

Reprogramming Roadblocks Are System Dependent.

Stem cell reports

Chantzoura E,Skylaki S,Menendez S,Kim SI,Johnsson A,Linnarsson S,Woltjen K,Chambers I,Kaji K

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Flow Cytometry

Tue Sep 08 00:00:00 EDT 2015

Epithelial-to-mesenchymal transition induces cell cycle arrest and parenchymal damage in renal fibrosis.

Nature medicine

Lovisa S,LeBleu VS,Tampe B,Sugimoto H,Vadnagara K,Carstens JL,Wu CC,Hagos Y,Burckhardt BC,Pentcheva-Hoang T,Nischal H,Allison JP,Zeisberg M,Kalluri R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Blocking the epithelial-to-mesenchymal transition pathway abrogates resistance to anti-folate chemotherapy in lung cancer.

Cell death & disease

Liang SQ,Marti TM,Dorn P,Froment L,Hall SR,Berezowska S,Kocher G,Schmid RA,Peng RW

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunofluorescence

Thu Jul 16 00:00:00 EDT 2015

Quantifying Memory CD8 T Cells Reveals Regionalization of Immunosurveillance.

Cell

Steinert EM,Schenkel JM,Fraser KA,Beura LK,Manlove LS,Igyártó BZ,Southern PJ,Masopust D

14-3249 was used in Immunofluorescence to indicate that most host cells are surveyed for reinfection by segregated residents rather than by recirculating cells that migrate throughout the blood and body.

Thu May 07 00:00:00 EDT 2015

Foxm1 regulates resolution of hyperoxic lung injury in newborns.

American journal of respiratory cell and molecular biology

Xia H,Ren X,Bolte CS,Ustiyan V,Zhang Y,Shah TA,Kalin TV,Whitsett JA,Kalinichenko VV

14-3249 was used in Flow cytometry/Cell sorting to demonstrate that Foxm1 influences pulmonary inflammatory responses to hyperoxia, inhibiting neutrophil-derived enzymes and enhancing monocytic responses that limit alveolar injury and remodeling in neonatal lungs.

Fri May 01 00:00:00 EDT 2015

Differentiation of human induced pluripotent stem cells to mature functional Purkinje neurons.

Scientific reports

Wang S,Wang B,Pan N,Fu L,Wang C,Song G,An J,Liu Z,Zhu W,Guan Y,Xu ZQ,Chan P,Chen Z,Zhang YA

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Immunocytochemistry

Wed Mar 18 00:00:00 EDT 2015

Single luminal epithelial progenitors can generate prostate organoids in culture.

Nature cell biology

Chua CW,Shibata M,Lei M,Toivanen R,Barlow LJ,Bergren SK,Badani KK,McKiernan JM,Benson MC,Hibshoosh H,Shen MM

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 14-3249-82) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

FGF2 mediates mouse spermatogonial stem cell self-renewal via upregulation of Etv5 and Bcl6b through MAP2K1 activation.

Development (Cambridge, England)

Ishii K,Kanatsu-Shinohara M,Toyokuni S,Shinohara T

14-3249 was used in Immunohistochemistry to assess the role of the FGF2 signalling pathway for SSC self-renewal.

Tue May 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: ARC-1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDH1; cell-CAM 120/80; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin

Gene Aliases: AA960649; Arc-1; Cadherin-1; CD324; CDH1; CDHE; E-cad; ECAD; L-CAM; LCAM; Um; UVO; Uvomorulin

UniProt ID: (Dog) F1PAA9, (Human) P12830, (Mouse) P09803

Entrez Gene ID: (Dog) 442858, (Human) 999, (Mouse) 12550

Molecular Function: cadherin

Function(s)

homophilic cell adhesion via plasma membrane adhesion molecules regulation of gene expression negative regulation of cell-cell adhesion negative regulation of cell migration adherens junction organization positive regulation of protein import into nucleus positive regulation of transcription, DNA-templated cellular response to lithium ion cellular response to indole-3-methanol protein localization to plasma membrane synapse assembly response to toxic substance single organismal cell-cell adhesion pituitary gland development extracellular matrix disassembly extracellular matrix organization neuron projection development response to drug positive regulation of transcription factor import into nucleus establishment of protein localization to plasma membrane in utero embryonic development trophectodermal cell differentiation epithelial cell morphogenesis cell adhesion sensory perception of sound calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules protein metabolic process regulation of protein localization regulation of water loss via skin regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation protein homooligomerization intestinal epithelial cell development salivary gland cavitation regulation of branching involved in salivary gland morphogenesis bicellular tight junction assembly cellular response to amino acid stimulus negative regulation of canonical Wnt signaling pathway cochlea development regulation of protein localization to cell surface regulation of neuron migration

Process(es)

protein binding beta-catenin binding ankyrin binding GTPase activating protein binding identical protein binding gamma-catenin binding glycoprotein binding calcium ion binding cell adhesion molecule binding cadherin binding involved in cell-cell adhesion protein phosphatase binding protein domain specific binding metal ion binding

CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), eBioscience™

Product Details

14-3249-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (FN)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

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Type

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Conjugate

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