Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	RM4-5
Conjugate	PE-Cyanine5 Alexa Fluor 532 Alexa Fluor 561 Alexa Fluor 647 Alexa Fluor 660 Alexa Fluor 700 APC APC-eFluor 780 Biotin Brilliant UV 395 Brilliant UV 496 Brilliant UV 563 Brilliant UV 615 Brilliant UV 661 Brilliant UV 737 Brilliant UV 805 Brilliant Violet 421 Brilliant Violet 650 Brilliant Violet 711 Brilliant Violet 786 eFluor 450 eFluor 506 eFluor 570 eFluor 615 FITC Pacific Orange
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_468698

Product Specific Information

Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Applications Reported: The RM4-5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The RM4-5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

CD226 Deletion Reduces Type 1 Diabetes in the NOD Mouse by Impairing Thymocyte Development and Peripheral T Cell Activation.

Frontiers in immunology

Shapiro MR,Yeh WI,Longfield JR,Gallagher J,Infante CM,Wellford S,Posgai AL,Atkinson MA,Campbell-Thompson M,Lieberman SM,Serreze DV,Geurts AM,Chen YG,Brusko TM

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Wed May 26 00:00:00 EDT 2021

Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials.

EBioMedicine

Okada R,Furusawa A,Vermeer DW,Inagaki F,Wakiyama H,Kato T,Nagaya T,Choyke PL,Spanos WC,Allen CT,Kobayashi H

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Dietary Glucose Consumption Promotes RALDH Activity in Small Intestinal CD103⁺CD11b⁺ Dendritic Cells.

Frontiers in immunology

Ko HJ,Hong SW,Verma R,Jung J,Lee M,Kim N,Kim D,Surh CD,Kim KS,Rudra D,Im SH

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Mon Apr 19 00:00:00 EDT 2021

Methnaridine is an orally bioavailable, fast-killing and long-acting antimalarial agent that cures Plasmodium infections in mice.

British journal of pharmacology

Wang W,Yao J,Chen Z,Sun Y,Shi Y,Wei Y,Zhou H,Yu Y,Li S,Duan L

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Mutation of an L-Type Calcium Channel Gene Leads to T Lymphocyte Dysfunction.

Frontiers in immunology

Fenninger F,Han J,Stanwood SR,Nohara LL,Arora H,Choi KB,Munro L,Pfeifer CG,Shanina I,Horwitz MS,Jefferies WA

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Thu Oct 29 00:00:00 EDT 2020

IL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury.

Frontiers in neurology

Gao W,Li F,Zhou Z,Xu X,Wu Y,Zhou S,Yin D,Sun D,Xiong J,Jiang R,Zhang J

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Impact of supplementation with a food-derived microbial community on obesity-associated inflammation and gut microbiota composition.

Genes & nutrition

Roselli M,Devirgiliis C,Zinno P,Guantario B,Finamore A,Rami R,Perozzi G

15-0042 was used in Flow cytometry/Cell sorting to study, using a mouse model, the effects of supplementary dietary microbes on obesity-associated inflammation and gut microbiota composition.

Thu Oct 01 00:00:00 EDT 2020

Increased Tim-3 expression on TILs during treatment with the Anchored GM-CSF vaccine and anti-PD-1 antibodies is inversely correlated with response in prostate cancer.

Journal of Cancer

Zhang X,Chen H,Li G,Zhou X,Shi Y,Zou F,Chen Y,Gao J,Yang S,Wu S,Long Z

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Thu Aug 27 00:00:00 EDT 2020

Critical Roles of E2F3 in Growth and Musculo-skeletal Phenotype in Mice.

International journal of medical sciences

Kim HR,Rahman FU,Kim KS,Kim EK,Cho SM,Lee K,Moon OS,Seo YW,Yoon WK,Won YS,Kang H,Kim HC,Nam KH

15-0042-82 was used in Flow Cytometry to suggest that E2F3 has a pivotal role in muscle and bone development, and affect normal mouse growth.

Thu Apr 30 00:00:00 EDT 2020

Protein arginine methyltransferase 5 promotes cholesterol biosynthesis-mediated Th17 responses and autoimmunity.

The Journal of clinical investigation

Webb LM,Sengupta S,Edell C,Piedra-Quintero ZL,Amici SA,Miranda JN,Bevins M,Kennemer A,Laliotis G,Tsichlis PN,Guerau-de-Arellano M

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Loss of testosterone impairs anti-tumor neutrophil function.

Nature communications

Markman JL,Porritt RA,Wakita D,Lane ME,Martinon D,Noval Rivas M,Luu M,Posadas EM,Crother TR,Arditi M

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Tue Mar 31 00:00:00 EDT 2020

Systemic inhibition of tissue-nonspecific alkaline phosphatase alters the brain-immune axis in experimental sepsis.

Scientific reports

Brichacek AL,Benkovic SA,Chakraborty S,Nwafor DC,Wang W,Jun S,Dakhlallah D,Geldenhuys WJ,Pinkerton AB,Millán JL,Brown CM

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Wed Dec 11 00:00:00 EST 2019

A unique mutator phenotype reveals complementary oncogenic lesions leading to acute leukemia.

JCI insight

Yin M,Baslan T,Walker RL,Zhu YJ,Freeland A,Matsukawa T,Sridharan S,Nussenzweig A,Pruitt SC,Lowe SW,Meltzer PS,Aplan PD

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Thu Dec 05 00:00:00 EST 2019

Clonal copy-number mosaicism in autoreactive T lymphocytes in diabetic NOD mice.

Genome research

Alriyami M,Marchand L,Li Q,Du X,Olivier M,Polychronakos C

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

Chloroquine modulates inflammatory autoimmune responses through Nurr1 in autoimmune diseases.

Scientific reports

Park TY,Jang Y,Kim W,Shin J,Toh HT,Kim CH,Yoon HS,Leblanc P,Kim KS

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Tue Oct 29 00:00:00 EDT 2019

Quantitative Methods for Measuring Repair Rates and Innate-Immune Cell Responses in Wounded Mouse Skin.

Frontiers in immunology

Li Z,Gothard E,Coles MC,Ambler CA

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Wed Mar 27 00:00:00 EDT 2019

Ufbp1 promotes plasma cell development and ER expansion by modulating distinct branches of UPR.

Nature communications

Zhu H,Bhatt B,Sivaprakasam S,Cai Y,Liu S,Kodeboyina SK,Patel N,Savage NM,Sharma A,Kaufman RJ,Li H,Singh N

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Wed Mar 06 00:00:00 EST 2019

Innate Sensing through Mesenchymal TLR4/MyD88 Signals Promotes Spontaneous Intestinal Tumorigenesis.

Cell reports

Koliaraki V,Chalkidi N,Henriques A,Tzaferis C,Polykratis A,Waisman A,Muller W,Hackam DJ,Pasparakis M,Kollias G

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Tue Jan 15 00:00:00 EST 2019

N-Cadherin-Expressing Bone and Marrow Stromal Progenitor Cells Maintain Reserve Hematopoietic Stem Cells.

Cell reports

Zhao M,Tao F,Venkatraman A,Li Z,Smith SE,Unruh J,Chen S,Ward C,Qian P,Perry JM,Marshall H,Wang J,He XC,Li L

15-0042 was used in Flow cytometry/Cell sorting to report that N-cad+ cells were functional bone and marrow stromal progenitor cells, giving rise to osteoblasts, adipocytes, and chondrocytes both in vitro and in vivo.

Tue Jan 15 00:00:00 EST 2019

Protease-activated receptor-2 signaling through β-arrestin-2 mediates Alternaria alkaline serine protease-induced airway inflammation.

American journal of physiology. Lung cellular and molecular physiology

Yee MC,Nichols HL,Polley D,Saifeddine M,Pal K,Lee K,Wilson EH,Daines MO,Hollenberg MD,Boitano S,DeFea KA

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

T-Cell-Intrinsic Receptor Interacting Protein 2 Regulates Pathogenic T Helper 17 Cell Differentiation.

Immunity

Shimada K,Porritt RA,Markman JL,O'Rourke JG,Wakita D,Noval Rivas M,Ogawa C,Kozhaya L,Martins GA,Unutmaz D,Baloh RH,Crother TR,Chen S,Arditi M

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Tue Nov 20 00:00:00 EST 2018

Modifying the cancer-immune set point using vaccinia virus expressing re-designed interleukin-2.

Nature communications

Liu Z,Ge Y,Wang H,Ma C,Feist M,Ju S,Guo ZS,Bartlett DL

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Thu Nov 08 00:00:00 EST 2018

Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5.

The Journal of experimental medicine

Buxadé M,Huerga Encabo H,Riera-Borrull M,Quintana-Gallardo L,López-Cotarelo P,Tellechea M,Martínez-Martínez S,Redondo JM,Martín-Caballero J,Flores JM,Bosch E,Rodríguez-Fernández JL,Aramburu J,López-Rodríguez C

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Mon Nov 05 00:00:00 EST 2018

Immunological effects of a low-dose cytarabine, aclarubicin and granulocyte-colony stimulating factor priming regimen on a mouse leukemia model.

Oncology letters

Chen J,Yang N,Liu H,Yao H,Wang J,Yang Y,Zhang W

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Granulocyte-macrophage colony-stimulating factor neuroprotective activities in Alzheimer's disease mice.

Journal of neuroimmunology

Kiyota T,Machhi J,Lu Y,Dyavarshetty B,Nemati M,Yokoyama I,Mosley RL,Gendelman HE

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Fri Jun 15 00:00:00 EDT 2018

Retinoid-Sensitive Epigenetic Regulation of the Hoxb Cluster Maintains Normal Hematopoiesis and Inhibits Leukemogenesis.

Cell stem cell

Qian P,De Kumar B,He XC,Nolte C,Gogol M,Ahn Y,Chen S,Li Z,Xu H,Perry JM,Hu D,Tao F,Zhao M,Han Y,Hall K,Peak A,Paulson A,Zhao C,Venkatraman A,Box A,Perera A,Haug JS,Parmely T,Li H,Krumlauf R,Li L

15-0042 was used in Flow cytometry/Cell sorting to demonstrate roles for retinoid signaling and the DERARE in maintaining HSCs and preventing leukemogenesis by coordinate regulation of Hoxb genes.

Thu May 03 00:00:00 EDT 2018

Protective function of Bu Shen Huo Xue formula on the immunity of B6AF1 mice with experimental autoimmune premature ovarian failure.

Experimental and therapeutic medicine

Wang P,Lu Y,Chen S,Chen Y,Hu C,Zuo Y

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Rap1 deficiency-provoked paracrine dysfunction impairs immunosuppressive potency of mesenchymal stem cells in allograft rejection of heart transplantation.

Cell death & disease

Ding Y,Liang X,Zhang Y,Yi L,Shum HC,Chen Q,Chan BP,Fan H,Liu Z,Tergaonkar V,Qi Z,Tse HF,Lian Q

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Wed Mar 07 00:00:00 EST 2018

Bryostatin-1 alleviates experimental multiple sclerosis.

Proceedings of the National Academy of Sciences of the United States of America

Kornberg MD,Smith MD,Shirazi HA,Calabresi PA,Snyder SH,Kim PM

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Tue Feb 27 00:00:00 EST 2018

IL-7-dependent STAT1 activation limits homeostatic CD4+ T cell expansion.

JCI insight

Le Saout C,Luckey MA,Villarino AV,Smith M,Hasley RB,Myers TG,Imamichi H,Park JH,O'Shea JJ,Lane HC,Catalfamo M

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Thu Nov 16 00:00:00 EST 2017

Discrimination of the heterogeneity of bone marrow‑derived dendritic cells.

Molecular medicine reports

Wang J,Dai X,Hsu C,Ming C,He Y,Zhang J,Wei L,Zhou P,Wang CY,Yang J,Gong N

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Ubiquitination of tumor suppressor PML regulates prometastatic and immunosuppressive tumor microenvironment.

The Journal of clinical investigation

Wang YT,Chen J,Chang CW,Jen J,Huang TY,Chen CM,Shen R,Liang SY,Cheng IC,Yang SC,Lai WW,Cheng KH,Hsieh TS,Lai MZ,Cheng HC,Wang YC,Chen RH

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Effect of follicular helper T cells on the pathogenesis of asthma.

Experimental and therapeutic medicine

Ma X,Sun Z,Zhai P,Yu W,Wang T,Li F,Ding J

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Batf3-dependent CD8α⁺ Dendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages.

EBioMedicine

Li Y,Liu X,Duan W,Tian H,Zhu G,He H,Yao S,Yi S,Song W,Tang H

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Immunological effects of vaccines combined with granulocyte colony-stimulating factor on a murine WEHI-3 leukemia model.

Oncology letters

Chen J,Zhang M,Zhou F,Wang J,Niu B,Zhang W

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting.

Scientific reports

Atkin-Smith GK,Paone S,Zanker DJ,Duan M,Phan TK,Chen W,Hulett MD,Poon IK

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Fri Jan 06 00:00:00 EST 2017

Context-dependent regulation of Th17-associated genes and IFNγ expression by the transcription factor NFAT5.

Immunology and cell biology

Alberdi M,Iglesias M,Tejedor S,Merino R,López-Rodríguez C,Aramburu J

15-0042 was used in Flow cytometry/Cell sorting to show that NFAT5 can modulate different T-cell responses depending on stress conditions and stimulatory context.

Sun Jan 01 00:00:00 EST 2017

A tumor lysate is an effective vaccine antigen for the stimulation of CD4(+) T-cell function and subsequent induction of antitumor immunity mediated by CD8(+) T cells.

Cancer biology & therapy

Kawahara M,Takaku H

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Mon Sep 12 00:00:00 EDT 2016

CD69 controls the uptake of L-tryptophan through LAT1-CD98 and AhR-dependent secretion of IL-22 in psoriasis.

Nature immunology

Cibrian D,Saiz ML,de la Fuente H,Sánchez-Díaz R,Moreno-Gonzalo O,Jorge I,Ferrarini A,Vázquez J,Punzón C,Fresno M,Vicente-Manzanares M,Daudén E,Fernández-Salguero PM,Martín P,Sánchez-Madrid F

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells.

Nature communications

Baptista MA,Keszei M,Oliveira M,Sunahara KK,Andersson J,Dahlberg CI,Worth AJ,Liedén A,Kuo IC,Wallin RP,Snapper SB,Eidsmo L,Scheynius A,Karlsson MC,Bouma G,Burns SO,Forsell MN,Thrasher AJ,Nylén S,Westerberg LS

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Mon Jul 18 00:00:00 EDT 2016

Exploring the induction of preproinsulin-specific Foxp3(+) CD4(+) Treg cells that inhibit CD8(+) T cell-mediated autoimmune diabetes by DNA vaccination.

Scientific reports

Stifter K,Schuster C,Schlosser M,Boehm BO,Schirmbeck R

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Mon Jul 11 00:00:00 EDT 2016

Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells.

eLife

Allison KA,Sajti E,Collier JG,Gosselin D,Troutman TD,Stone EL,Hedrick SM,Glass CK

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Mon Jul 04 00:00:00 EDT 2016

STAT3/5-Dependent IL9 Overexpression Contributes to Neoplastic Cell Survival in Mycosis Fungoides.

Clinical cancer research : an official journal of the American Association for Cancer Research

Vieyra-Garcia PA,Wei T,Naym DG,Fredholm S,Fink-Puches R,Cerroni L,Odum N,O'Malley JT,Gniadecki R,Wolf P

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model.

Molecular medicine reports

Yue Y,Li P,Song N,Li B,Li Z,Guo Y,Zhang W,Wei MQ,Gai Z,Meng H,Wang J,Qin L

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Sun May 01 00:00:00 EDT 2016

Sex bias in CNS autoimmune disease mediated by androgen control of autoimmune regulator.

Nature communications

Zhu ML,Bakhru P,Conley B,Nelson JS,Free M,Martin A,Starmer J,Wilson EM,Su MA

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Wed Apr 13 00:00:00 EDT 2016

Immunogenicity and protective efficacy of DMT liposome-adjuvanted tuberculosis subunit CTT3H vaccine.

Human vaccines & immunotherapeutics

Teng X,Tian M,Li J,Tan S,Yuan X,Yu Q,Jing Y,Zhang Z,Yue T,Zhou L,Fan X

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Fri Mar 11 00:00:00 EST 2016

Irradiation of necrotic cancer cells, employed for pulsing dendritic cells (DCs), potentiates DC vaccine-induced antitumor immunity against high-grade glioma.

Oncoimmunology

Vandenberk L,Garg AD,Verschuere T,Koks C,Belmans J,Beullens M,Agostinis P,De Vleeschouwer S,Van Gool SW

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Oligodendrocyte death results in immune-mediated CNS demyelination.

Nature neuroscience

Traka M,Podojil JR,McCarthy DP,Miller SD,Popko B

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.

Nature communications

Lim S,Kim WJ,Kim YH,Lee S,Koo JH,Lee JA,Yoon H,Kim DH,Park HJ,Kim HM,Lee HG,Yun Kim J,Lee JU,Hun Shin J,Kyun Kim L,Doh J,Kim H,Lee SK,Bothwell ALM,Suh M,Choi JM

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Tue Sep 15 00:00:00 EDT 2015

Single-Dose CpG Immunization Protects Against a Heterosubtypic Challenge and Generates Antigen-Specific Memory T Cells.

Frontiers in immunology

Vogel AJ,Brown DM

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Fri Jul 10 00:00:00 EDT 2015

Leukocyte population dynamics and detection of IL-9 as a major cytokine at the mouse fetal-maternal interface.

PloS one

Habbeddine M,Verbeke P,Karaz S,Bobé P,Kanellopoulos-Langevin C

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Wed Jun 10 00:00:00 EDT 2015

Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice.

Cardiovascular research

Wan W,Liu Q,Lionakis MS,Marino AP,Anderson SA,Swamydas M,Murphy PM

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Recombinant adeno-vaccine expressing enterovirus 71-like particles against hand, foot, and mouth disease.

PLoS neglected tropical diseases

Tsou YL,Lin YW,Shao HY,Yu SL,Wu SR,Lin HY,Liu CC,Huang C,Chong P,Chow YH

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Characterization of two distinct lymphoproliferative diseases caused by ectopic expression of the Notch ligand DLL4 on T cells.

PloS one

Xiong H,Maraver A,Latkowski JA,Henderson T,Schlessinger K,Ding Y,Shen J,Tadokoro CE,Lafaille JJ

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Mon Feb 23 00:00:00 EST 2015

The efficacy of tumor debulking surgery is improved by adjuvant immunotherapy using imiquimod and anti-CD40.

BMC cancer

Khong A,Cleaver AL,Fahmi Alatas M,Wylie BC,Connor T,Fisher SA,Broomfield S,Lesterhuis WJ,Currie AJ,Lake RA,Robinson BW

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Wed Dec 17 00:00:00 EST 2014

SH2D2A modulates T cell mediated protection to a B cell derived tumor in transgenic mice.

PloS one

Berge T,Grønningsæter IH,Lorvik KB,Abrahamsen G,Granum S,Sundvold-Gjerstad V,Corthay A,Bogen B,Spurkland A

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Tue Aug 26 00:00:00 EDT 2014

Nod2 activates NF-kB in CD4+ T cells but its expression is dispensable for T cell-induced colitis.

PloS one

Zanello G,Goethel A,Forster K,Geddes K,Philpott DJ,Croitoru K

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Thu Jul 31 00:00:00 EDT 2014

TLR2 engagement on CD4(+) T cells enhances effector functions and protective responses to Mycobacterium tuberculosis.

European journal of immunology

Reba SM,Li Q,Onwuzulike S,Ding X,Karim AF,Hernandez Y,Fulton SA,Harding CV,Lancioni CL,Nagy N,Rodriguez ME,Wearsch PA,Rojas RE

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Thu May 01 00:00:00 EDT 2014

Agonistic induction of PPARγ reverses cigarette smoke-induced emphysema.

The Journal of clinical investigation

Shan M,You R,Yuan X,Frazier MV,Porter P,Seryshev A,Hong JS,Song LZ,Zhang Y,Hilsenbeck S,Whitehead L,Zarinkamar N,Perusich S,Corry DB,Kheradmand F

15-0042 was used in Flow cytometry/Cell sorting to reveal a proinflammatory pathway, in which reduced PPARγ activity promotes emphysema.

Sat Mar 01 00:00:00 EST 2014

Mucosal and systemic T cell response in mice intragastrically infected with Neospora caninum tachyzoites.

Veterinary research

Correia A,Ferreirinha P,Costa AA,Dias J,Melo J,Costa R,Ribeiro A,Faustino A,Teixeira L,Rocha A,Vilanova M

15-0042 was used in Flow cytometry/Cell sorting to indicate that TCRαβ+ CD8+ cells contribute to host protection by producing IFN-γ during gastrointestinal infection with Neospora caninum tachyzoites.

Sat Aug 10 00:00:00 EDT 2013

Trypanosoma cruzi trans-sialidase initiates a program independent of the transcription factors RORγt and Ahr that leads to IL-17 production by activated B cells.

Nature immunology

Bermejo DA,Jackson SW,Gorosito-Serran M,Acosta-Rodriguez EV,Amezcua-Vesely MC,Sather BD,Singh AK,Khim S,Mucci J,Liggitt D,Campetella O,Oukka M,Gruppi A,Rawlings DJ

15-0042 was used in Flow cytometry/Cell sorting to study how the generation of IL-17 B cells may be a feature of innate immune responses required for pathogen control or IL-17-mediated autoimmunity.

Wed May 01 00:00:00 EDT 2013

Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice.

Cardiovascular research

Wan W,Lionakis MS,Liu Q,Roffê E,Murphy PM

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

The stalk domain and the glycosylation status of the activating natural killer cell receptor NKp30 are important for ligand binding.

The Journal of biological chemistry

Hartmann J,Tran TV,Kaudeer J,Oberle K,Herrmann J,Quagliano I,Abel T,Cohnen A,Gatterdam V,Jacobs A,Wollscheid B,Tampé R,Watzl C,Diefenbach A,Koch J

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Fri Sep 07 00:00:00 EDT 2012

Evaluation of Lassa virus vaccine immunogenicity in a CBA/J-ML29 mouse model.

Vaccine

Goicochea MA,Zapata JC,Bryant J,Davis H,Salvato MS,Lukashevich IS

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2012

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using CD4 monoclonal antibody (Product # 15-0042-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

Limited role of secreted aspartyl proteinases Sap1 to Sap6 in Candida albicans virulence and host immune response in murine hematogenously disseminated candidiasis.

Infection and immunity

Correia A,Lermann U,Teixeira L,Cerca F,Botelho S,da Costa RM,Sampaio P,Gärtner F,Morschhäuser J,Vilanova M,Pais C

15-0042 was used in Flow cytometry/Cell sorting to investigate the role of secreted aspartyl proteinases Sap1 to Sap6 in Candida albicans virulence in a murine model of hematogenously disseminated candidiasis.

Mon Nov 01 00:00:00 EDT 2010

Lack of TNFR2 expression by CD4(+) T cells exacerbates experimental colitis.

European journal of immunology

Dayer Schneider J,Seibold I,Saxer-Sekulic N,Paredes BE,Saurer L,Mueller C

15-0042 was used in Flow cytometry/Cell sorting to assess the role of TNF receptor 2 signalling in the CD4(+) T-cell transfer model of colitis.

Wed Jul 01 00:00:00 EDT 2009

Flow-cytometric phosphoprotein analysis reveals agonist and temporal differences in responses of murine hematopoietic stem/progenitor cells.

PloS one

Kalaitzidis D,Neel BG

15-0042 was used in Flow cytometry/Cell sorting to develop a method to study intracellular signaling in murine HSC/progenitor cell (HPC)-enriched populations, showing agonist and temporal differences in responses of HSC/HPC.

Tue Feb 10 00:00:00 EST 2009

IL-10 regulates movement of intestinally derived CD4+ T cells to the liver.

Journal of immunology (Baltimore, Md. : 1950)

Bliss SK,Bliss SP,Beiting DP,Alcaraz A,Appleton JA

Fri Jun 15 00:00:00 EDT 2007

The impact of altered p53 dosage on hematopoietic stem cell dynamics during aging.

Blood

Dumble M,Moore L,Chambers SM,Geiger H,Van Zant G,Goodell MA,Donehower LA

15-0042 was used in Flow cytometry/Cell sorting to study how altering p53 dosage during ageing impacts hematopoietic stem cell dynamics.

Thu Feb 15 00:00:00 EST 2007

Cutaneous gene expression by DNA microarray in murine sclerodermatous graft-versus-host disease, a model for human scleroderma.

The Journal of investigative dermatology

Zhou L,Askew D,Wu C,Gilliam AC

15-0042 was used in Flow cytometry/Cell sorting to find consistent differences in inflammatory patterns, between mice with sclerodermatous graft-versus-host disease and controls in cytokine messenger RNAs.

Thu Feb 01 00:00:00 EST 2007

Converting IL-15 to a superagonist by binding to soluble IL-15R{alpha}.

Proceedings of the National Academy of Sciences of the United States of America

Rubinstein MP,Kovar M,Purton JF,Cho JH,Boyman O,Surh CD,Sprent J

Tue Jun 13 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: CD4; CD4 antigen p55; cd4a; fCD4; Leu-3; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)

Gene Aliases: Cd4; L3T4; Ly-4

UniProt ID: (Mouse) P06332

Entrez Gene ID: (Mouse) 12504

Function(s)