Invitrogen

CD4 Monoclonal Antibody (RM4-5), PE-Cyanine5, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

40 Published Figures

44 References

View all (374) CD4 antibodies

Datasheet

Datasheet

Product Image Gallery

CD4 Antibody — Fig. 3 Immune status change in the tumour microenvironment post-virus treatments. B6 mice were inoculated i.p. with 5 x 10 5 MC38-luc cells and treated with PBS, vvDD, vvDD-IL-2-FG, or vvDD-IL-2-RG at 2 x 10 8 PFU per mouse 9 days post-tumour inoculation. Tumour-bearing mice were sacrificed 5 days post-treatment and primary tumours were collected and analysed by flow cytometry to determine CD4 + Foxp3 - ( a ) and CD8 + IFN-gamma + T cells ( b ), exhausted CD8 + T cell ( c-f ), memory-phenotype T cells (CD8 + CD44 hi ) ( g ), regulatory T cells (CD4 + Foxp3 + ) ( h ), CD8/Treg ( i ), or by RT-qPCR to determine IFN-gamma, granzyme B, perforin, CXCL9, TGF-beta, CD105, and VEGF ( j-p ). Four to five mice were used for each treatment group and data are representative of two independent experiments ( a - i ) or combined from three independent experiments ( j-m ) or two independent experiments ( n-p ). In a separate experiment, B6 mice were i.p. inoculated with 5 x 10 5 MC38-luc cells and treated with vvDD-IL-2-RG or PBS 9 days post-tumour inoculation. Anti-CD8 Ab (250 ug per injection), anti-CD4 Ab (150 ug per injection), anti-IFN-gamma Ab (200 ug per injection) (nine mice per group) ( q ), or PK136 (300 ug per injection) ( s ), were i.p. injected into mice to deplete CD8 + T cells, CD4 + T cells or neutralise circulating IFN-gamma, or NK1.1 + cells, and a log-rank (Mantel-Cox) test was used to compare survival rates ( r , t ), respectively. * P <0.05; ** P <0.01; *** P <0

CD4 Antibody in Flow Cytometry (Flow) — Staining of BALB/c splenocytes with 0.06 µg of Rat IgG2a K Isotype Control PE-Cyanine5 (Product # 15-4321-80) (open histogram) or 0.06 µg of Anti-Mouse CD4 PE-Cyanine5 (filled histogram).Total cells were used for analysis.

CD4 Antibody in Relative expression — Staining of mouse splenocytes and bone marrow cells. Right: As expected based on known relative expression patterns, CD4 clone RM4-5 stains a subset of splenocytes and does not stain any bone marrow cells. Details: Balb/c bone marrow cells (left) and splenocytes (middle) were surface stained with CD4 (clone RM4-5) followed by staining with 7-AAD. Viable bone marrow cells in the lymphoid (blue histogram) and myeloid (purple histogram) gates and viable splenocytes (orange histogram) were used for analysis. {RE}

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.125 µg/test

Published Applications

Flow Cytometry (Flow)

See 44 publications below

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host / Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RM4-5

Conjugate

PE-Cyanine5

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_468698

Product Specific Information

Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Applications Reported: The RM4-5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The RM4-5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD4; CD4 antigen p55; CD4 receptor; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)

Gene Aliases: Cd4; L3T4; Ly-4

UniProt ID: (Mouse) P06332

Entrez Gene ID: (Mouse) 12504

Molecular Function: cytokine receptor defense/immunity protein immunoglobulin receptor superfamily receptor