|Tested species reactivity||Human, Mouse, Non-human primate|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human CDH1 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/200 - 1/400|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA5-15711 targets CDH1 in FACS, IHC, and WB applications and shows reactivity with Human, mouse, and Non-human primate samples.
The MA5-15711 immunogen is purified recombinant fragment of human CDH1 expressed in E. Coli.
MA5-15711 detects CDH1 which has a predicted molecular weight of approximately 135kDa.
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Ablation of Doublecortin-Like Kinase 1 in the Colonic Epithelium Exacerbates Dextran Sulfate Sodium-Induced Colitis.
MA5-15711 was used in western blot to investigate the involvement of doublecortin-like kinase 1 in colitis
|Qu D,Weygant N,May R,Chandrakesan P,Madhoun M,Ali N,Sureban SM,An G,Schlosser MJ,Houchen CW||PloS one (10:null)||2015|