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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Her-2 (ErbB2) Antibodies
          The product (cat # {{productObj.productId}}) that you're looking for has been discontinued and replaced with {{productObj.replacementProductId}}. The product (cat # {{productObj.productId}}) that you're looking for has been discontinued. Please contact Technical Support with any questions.

          Invitrogen

          ErbB2 (HER-2) Monoclonal Antibody (3B5)

          Advanced Verification
          7 Published Figures
          62 References
          View all (281) Her-2 (ErbB2) antibodies
          Datasheet
          Protocols
          Datasheet
          Protocols

          Cite ErbB2 (HER-2) Monoclonal Antibody (3B5)

          • Antibody Testing Data (3)
          • Published Figures (7)
          • Advanced Verification (1)
          ErbB2 (HER-2) Antibody in Immunocytochemistry (ICC/IF)
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          ErbB2 (HER-2) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 11

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          ErbB2 (HER-2) Antibody (MA5-13675) in ICC/IF

          Immunofluorescent analysis of HER-2/ErbB2 (green) showing membrane staining in SK-BR-3 cells (right) compared to a negative control without primary antibody (left). Cells were was permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a HER-2/ErbB2 monoclonal antibody (Product # MA5-13675) in 3% BSA-PBS at a dilutio... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          ErbB2 (HER-2) Antibody in Immunocytochemistry (ICC/IF)
          ErbB2 (HER-2) Antibody in Immunoprecipitation (IP)
          ErbB2 (HER-2) Antibody in Immunoprecipitation (IP)
          ErbB2 (HER-2) Antibody in Immunohistochemistry (IHC)
          ErbB2 (HER-2) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          ErbB2 (HER-2) Antibody in Western Blot (WB)
          ErbB2 (HER-2) Antibody in Western Blot (WB)
          ErbB2 (HER-2) Antibody in Western Blot (WB)
          ErbB2 (HER-2) Antibody in Western Blot (WB)
          ErbB2 (HER-2) Antibody in ELISA (ELISA)
          ErbB2 (HER-2) Antibody

          Product Details

          MA5-13675

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 19 publications 19 publications

          Immunohistochemistry (IHC)

          1:1,000-1:2,000
          View 30 publications 30 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          View 5 publications 5 publications

          Flow Cytometry (Flow)

          1:10-1:100
          -

          ELISA (ELISA)

          -
          View 4 publications 4 publications

          Immunoprecipitation (IP)

          1:25
          View 3 publications 3 publications
          Product Specifications

          Published species

          Avian, Cat, Hamster, Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          3B5

          Immunogen

          A synthetic peptide from the C-terminus of human c-erbB-2 protein. This Sequence Is Identical In Rat Neu Protein.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Biotin 
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.1 mg/mL

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10985617

          Product Specific Information

          MA5-13675 targets HER-2 in IHC, IP, ICC, IF, FACS and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.

          The MA5-13675 immunogen is a synthetic peptide from the C-terminus of human c-erbB-2 protein. This Sequence Is Identical In Rat Neu Protein.

          Target Information

          ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
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          We're here to help

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