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Mouse brain sections were labeled with the NeuroTrace® 500/525 green-fluorescent Nissl stain (Cat. no. N21480) or with our biotin-XX conjugate of anti-HuC/HuD antibody, followed by visualization with Alexa Fluor® 568 streptavidin (Cat. no. S11226). Both sections were counterstained with DAPI (Cat. no. D1306, D3571, D21490) (Cat. No. A21272)
|Tested species reactivity||Human|
|Published species reactivity||Zebra fish , Human , Mouse|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Human HuC/HuD neuronal protein.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1-5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition of selective neural tissue injury associated with small cell lung carcinoma. The antibodies bind specifically to antigens present exclusively in neuronal cells and are thus useful as markers of neuronal cells in tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The enteric nervous system of P2Y13 receptor null mice is resistant against high-fat-diet- and palmitic-acid-induced neuronal loss.
A-21272 was used in immunocytochemistry to study the role of the ADP-sensitive P2Y3 receptor in lipid-induced enteric neuropathy.
|Voss U,Turesson MF,Robaye B,Boeynaems JM,Olde B,Erlinge D,Ekblad E||Purinergic signalling (10:455)||2014|
Serotonin receptor diversity in the human colon: Expression of serotonin type 3 receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E.
A-21272 was used in immunohistochemistry to study the expression of serotonin receptor subtypes in the human colon.
|Kapeller J,Möller D,Lasitschka F,Autschbach F,Hovius R,Rappold G,Brüss M,Gershon MD,Niesler B||The Journal of comparative neurology (519:420)||2011|
Conditional gene expression and lineage tracing of tuba1a expressing cells during zebrafish development and retina regeneration.
A-21272 was used in immunohistochemistry to investigate CNS regeneration in zebrafish
|Ramachandran R,Reifler A,Parent JM,Goldman D||The Journal of comparative neurology (518:4196)||2010|
Quantitative assessment of glial cells in the human and guinea pig enteric nervous system with an anti-Sox8/9/10 antibody.
A-21272 was used in immunohistochemistry to quantitate glial cells in the human and guinea pig enteric nervous system.
|Hoff S,Zeller F,von Weyhern CW,Wegner M,Schemann M,Michel K,Rühl A||The Journal of comparative neurology (509:356)||2008|
HUCL, PLE21, PNEM, HUC, HUD
ELAV-like protein 3, Hu antigen C, hu-antigen C, paraneoplastic cerebellar degeneration-associated antigen, paraneoplastic limbic encephalitis antigen 21, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D), ELAV-like protein 4, Embryonic lethal, abnormal vision, Drosophila, homolog of, like-4, Hu antigen D, hu-antigen D, paraneoplastic encephalomyelitis antigen HuD, HuC, ELAVL3, HUC, PLE21, HuD, ELAVL4, HUD, PNEM