Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 14-5971 should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.
Applications Reported: PK136 has been reported for use in flow cytometric analysis.
Applications Tested: The PK136 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product # 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product # A10497).
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
NK1.1 (Killer cell lectin-like receptor subfamily B, member 1, KLRB1, NKR-P1A, CD161, cluster of differentiation 161), refers to Natural Killer (NK) cells, lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein, NKR-P1A or CD161, is classified as a type II membrane protein because it has an external C terminus. NKR-P1A, the receptor encoded by the KLRB1 gene, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD161 antigen-like family member B; CD161 antigen-like family member C; CD161b; CD161c; Inhibitory receptor NKR-P1B; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1B allele A; Killer cell lectin-like receptor subfamily B member 1B allele B; Killer cell lectin-like receptor subfamily B member 1C; killer cell lectin-like receptor subfamily B member 1D; ly-55b; Ly-55c; Ly-55d; lymphocyte antigen 55 complex, locus B; lymphocyte antigen 55 complex, locus C; lymphocyte antigen 55 complex, locus D; lymphocyte antigen 55b; Lymphocyte antigen 55c; Lymphocyte antigen 55d; lymphocyte antigen 59; natural killer cell receptor protein NKR-P1B; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; Natural killer cell surface protein NKR-P1B allele B6; natural killer cell surface protein NKR-P1B allele SJL/BALB; Natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; NK1.1; NKR-P1 34; NKR-P1 40; NKR-P1.9; NKR-P1C; NKR-P1D
Gene Aliases: AI462337; CD161; Klrb1b; Klrb1c; Klrb1d; ly-55c; Ly-59; Ly55b; Ly55c; Ly55d; Ly59; Nk-1; Nk-1.2; NK-RP1; Nk1; NK1.1; Nk1.2; NKR-P1.9; NKR-P1B; NKR-P1D; NKRP1; Nkrp1-b; NKRP140; Nkrp1b; Nkrp1c; Nkrp1d