Invitrogen

SOX2 Monoclonal Antibody (Btjce), eBioscience™

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

40 Published Figures

50 References

View all (63) SOX2 antibodies

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SOX2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of SOX2 was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SOX2 Rat Antibody (Product # 14-9811-82, 1 µg/mL) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rat IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11006) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows SOX2 negative cell line HeLa with no signal. Panel f represents control cells with no primary to assess background. The images were captured at 60X magnification.

SOX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry on formalin-fixed paraffin embedded human testes, using 5 µg/mL Anti-Human/Mouse Sox2 Purified followed by biotinylated anti-rat IgG, and DAB visualization.Nuclei are counterstained with hematoxylin.

SOX2 Antibody in Western Blot (WB) — Western blot analysis was performed on modified whole cell lysate (1% SDS) (30 µg lysate) of NCCIT (Lane 1), NTERA-2 (Lane 2) and HeLa (Lane 3). The blot was probed with anti-SOX2 Monoclonal Antibody (Product # 14-9811-82, 1 µg/mL) and detected by chemiluminescence using F(ab')2-Rabbit anti-Rat IgG (H+L) Secondary Antibody, HRP (Product # PA1-29927, 0.25 µg/mL, 1:4000 dilution). A 35 kDa band corresponding to SOX2 was observed in NCCIT and NTERA-2 cells but not in HeLa which is reported to be negative for SOX2 expression.

SOX2 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Fig. 2. NSCs and adult hippocampal neurogenesis are unaffected by the NSC-specific deletion of beta-catenin and inhibition of Wnt/beta-catenin signalling. (A) Two-month-old control and beta-cat del ex2-6 mice were administered tamoxifen for 5 consecutive days and sacrificed 30 days after the first tamoxifen injection. (B) YFP, GFAP, SOX2 and Ki67 immunolabelling in the DG of control and beta-cat del ex2-6 mice 30 days after tamoxifen administration. White-dashed lines denote the SGZ. (C-F) Quantification of the data shown in B. C: proportion of Ki67+ NSCs (control versus beta-cat del ex2-6 : 3.33+-0.61% versus 3.333+-0.76%). D: total number of NSCs (YFP+ GFAP+ SOX2+ radial cells in the SGZ) normalised to the length of the SGZ (mm) (control versus beta-cat del ex2-6 : 21.2+-1.73 versus 23.56+-1.52). E: proportion of proliferating (Ki67+) IPCs (SOX2+ YFP+ GFAP- cells in the SGZ; control versus beta-cat del ex2-6 : 56+-4.56% versus 47.67+-4.03%). F: total number of IPCs normalised to the length of the SGZ (mm) (SOX2+ YFP+ GFAP- cells in the SGZ; control versus beta-cat del ex2-6 : 20.18+-2.31 versus 22.43+-2.62). n =6. (G) YFP, TBR2 and DCX immunolabelling in the DG of control and beta-cat del ex2-6 mice 30 days after tamoxifen administration. (H,I) Quantification of the data shown in G. H: total number of TBR2+ IPCs normalised to the SGZ length (mm) (TBR2+ YFP+ cells in the SGZ; control versus beta-cat del ex2-6 : 14.29+-1.51 versus 17.16+-3.09). I: total number of neuroblasts

SOX2 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Western Blot and Immunocytochemistry

SOX2 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Brain organoid generated by vertical mixing showed inverted structure in comparison with brain organoid generated by orbital mixing. a Schematic diagram of conditions used to induce brain organoid by orbital mixing (upper schema) and by vertical mixing (lower schema). b , c Immunostaining for neural progenitor (SOX2, magenta) and neuron (MAP2, green) in brain organoid generated by orbital mixing ( b ) or vertical mixing ( c ) on Day 56. d Quantification of SOX2-positive area in the peripheral region of brain organoid on Day 56. The peripheral region was defined as 100 um inside from the edge of brain organoid. Brain organoid from vertical mixing showed higher percentage of SOX2-positive area in peripheral region in comparison with brain organoid from orbital mixing. Data represent mean +- SD ( n = 6 for brain organoids by orbital mixing, n = 7 for brain organoids by vertical mixing). Difference between the two conditions was analyzed by Student's two-tailed t -test (* p < 0.0001). e , f Immunostaining for markers of neural progenitors (SOX2, blue), intermediate neural progenitors (TBR2, gray), and cortical neurons (CTIP2, red; MAP2, green) in brain organoids by orbital mixing ( e ) or vertical mixing ( f ) on Day 56. g , h Immunostaining for markers of ventricular neuroepithelial cells (N-CADHERIN: N-CAD, gray), neural progenitors (SOX2, magenta), and cortical neurons (MAP2, green) in brain organoids by orbital mixing ( g ) or vertical mixing ( h ) on Day 56. Note the apical

SOX2 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Compartmental cell identity is altered upon Hedgehog pathway inhibition. RT-qPCR for SOX2 ( A ), SOX9 ( B ), and ACTA2 ( C ) in fetal lung explants treated with 5E1 compared to control. Results are shown as mean +- SEM, * p < 0.05, n = 6 for each group. ( D - H ) Western blot analysis of protein expression of SOX2 ( D , E ), SOX9 ( F , G ), and ACTA2 ( H , I ) in 5E1 treated explants compared to control ( n = 3 for each condition). Western blot densitometry ratios are shown in ( E , G , I ) ( n = 3 for each condition). Results are shown as mean +- SEM. IF staining of fetal lung explants treated ( K ) or not ( J ) with 5E1 for SOX2 (green), SOX9 (white), and ACTA2 (red). Quantification of the number of epithelial positive SOX2 cells ( L ) and SOX9 ( M ) ( n = 4 for each group). * p < 0.05, ** p < 0.01; ns = no stress.

SOX2 Antibody in Immunohistochemistry (IHC) — Fig. 7. PD-1 on BTICs promotes intracranial tumor growth in mice. ( A ) Schematic diagram depicts implantation of Pdcd1 -knockdown or OE cells into the striatum of mice. Kaplan-Meier analysis to assess the survival of wild-type mice i4mplanted with ( B ) Pdcd1 knockdown or ( C ) overexpressing murine BT0309. Cells transduced with lentiviral vectors coding nontarget shRNA or transfected with empty PiggyBac vector were considered as controls for knockdown (shRNA control) or OE (OE control) BTICs, respectively. ( D ) Representative images of immunofluorescence staining of PD-1, SOX2, and p65 in sections collected from C57BL/6 wild-type mice implanted with Pdcd1 -knockdown or shRNA controls. Nuclear translocation (yellow arrowheads) and cytoplasmic localization (blue arrowheads) of p65 in BTICs are shown on the images. ( E ) Kaplan-Meier analysis of Rag1 KO mice implanted with Pdcd1 -knockdown or shRNA controls of mBT0309. Representative T2-weighted MRI images ( F ) at day 50 of Rag1 KO mice implanted with Pdcd1 -knockdown or ( G ) at day 54 of Rag1 KO mice implanted with Pdcd1 OE mBT0309 (tumor demarcated with red outlines). Quantitation of tumor volume from MRI images of mice implanted with ( H ) Pdcd1 knockdown or ( I ) OE BTICs versus respective vector controls. Results are representative of n = 2 to 3 independent experiments. Unpaired (two-tailed) t test was used to assess significance between two groups in (I). For more than two groups in (H), one-way ANOVA with Tukey's pos

SOX2 Antibody in Flow Cytometry (Flow) — Fig. 4 The phosphorylation of PTK6 promotes CRC cell stemness. A Western blot analyses were performed to verify the successful construction of FLAG-tagged empty vector, wild-type (WT), kinase-dead (KM) and constitutively active (YF) PTK6 recombinant mutants overexpression CRC cells. B In vitro limiting dilution assays show the effects of phosphorylated PTK6 on the formation of CSC spheres (mean +- SD, n = 12), likelihood ratio test. C Tumor sphere formation assays were performed to detect the sphere-forming ability of vector, PTK6 WT , PTK6 KM and PTK6 YF overexpression CRC cells (mean +- SD, n = 3). D Real-time q-PCR analysis shows the effects of phosphorylated PTK6 on the expression of stem cell markers in CRC cells (mean +- SD, n = 3). E Western blot assays were performed to detect the effects of phosphorylated PTK6 on the expression of stem cell markers in CRC cells. F The number of CD133 + or SOX2 + cells was evaluated in the vector, PTK6 WT , PTK6 KM and PTK6 YF overexpression CRC cells by flow cytometry (mean +- SD, n = 3). G Co-immunofluorescent staining of PTK6 (red), CD133 (green) and EPACAM (green) in CRC cells. Scale bar represents 5 mum. H Co-immunofluorescent staining of PTK6 (red), CD133 (green) and EPACAM (green) in CRC tumor spheres. Scale bar = 5 mum. I In vivo limiting dilution analysis was performed to detect the tumorigenicity of vector, PTK6 WT , PTK6 KM and PTK6 YF overexpression CRC cells in nude mice (mean +- SD, n = 7). J IHC staining was used to det

SOX2 Antibody — Knockdown of SOX2 was achieved by transfecting NTERA-2 cells with SOX2 specific siRNAs (Silencer® select Product # s13294, s13296). Western blot analysis (Fig. a) was performed using whole cell extracts from the SOX2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and un-transfected cells (lane 1). The blots were probed with SOX2 Monoclonal Antibody (Btjce), eBioscience™ Antibody (Product # 14-9811-82, 1 µg/mL) and F(ab')2-Rabbit anti-Rat IgG (H+L) Secondary Antibody, HRP (Product # PA1-29927, 0.25 µg/mL, 1:4,000 dilution). Densitometry analysis of this western blot is shown in histogram (Fig b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SOX2. {KD}

Product Details

14-9811-82

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/ml	View 3 publications 3 publications
Immunohistochemistry (IHC)	-	View 18 publications 18 publications
Immunohistochemistry (Paraffin) (IHC (P))	5 µg/mL	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	-	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	5 µg/mL	View 23 publications 23 publications
Flow Cytometry (Flow)	Assay-Dependent	View 3 publications 3 publications
ELISA (ELISA)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Bovine, Chicken, Human, Mouse, Non-human primate
Host/Isotype	Rat / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	Btjce
Conjugate	Unconjugated Alexa Fluor 488 eFluor 570 eFluor 660
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_11219471

Product Specific Information

Description: The Btjce monoclonal antibody reacts with the transcription factor Sox2, a member of the SOX (sex determining region Y -related HMG (High Mobility Group) Box) family of proteins. Sox family members play a role in early organ development, and in particular, Sox2 is essential for regulating genes that control normal mammalian embryogenesis. Sox2 and family member Sox3 are expressed as early as the preimplantation and epiblast stages respectively. Later expression is restricted to the neuroepithelium. Sox2 has been shown to be necessary for maintaining self-renewal and pluripotency of mouse and human embryonic stem (ES) cells (ESC). Oct4 (POU5F1), Klf4, c-myc, and Sox2 were the original four factors used to reprogram differentiated mouse and human cells to induced pluripotent stem cells (iPSC).

Expression of Sox2 is tightly regulated and recent studies have demonstrated that small changes in the levels of Sox2 in ES cells can trigger differentiation into multiple cell types. Sox2 expression is not limited to ES cells, it is also essential for early neurogenesis where its expression becomes restricted to the neural plate, and later to neural stem cells where it functions to suppress neural differentiation. Sox2 in combination with other stem cell markers can be used to characterize stem cell populations. Ectopic expression of Sox2 has been associated with multiple cancer types including colorectal and breast.

Applications Reported: This Btjce antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunocytochemistry, western blot and ELISA (Fluorochrome-conjugated Btjce is recommended for use in intracellular flow cytometry).

Applications Tested: This Btjce antibody has been tested by immunohistochemistry on FFPE human testes at less than or equal to 5 µg/mL. The antibody can be used with either high or low pH antigen retrieval. This Btjce antibody has been tested by immunocytochemistry on fixed and permeabilized iPS cells at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Bacterial expression of region after aa135 (homology between species but not to other family members)

Target Information

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hedgehog Signaling Pathway Orchestrates Human Lung Branching Morphogenesis.

International journal of molecular sciences

Belgacemi R,Danopoulos S,Deutsch G,Glass I,Dormoy V,Bellusci S,Al Alam D

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunohistochemistry

Mon May 09 00:00:00 EDT 2022

Hedgehog Signaling Pathway Orchestrates Human Lung Branching Morphogenesis.

International journal of molecular sciences

Belgacemi R,Danopoulos S,Deutsch G,Glass I,Dormoy V,Bellusci S,Al Alam D

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunohistochemistry

Mon May 09 00:00:00 EDT 2022

The Alzheimer susceptibility gene BIN1 induces isoform-dependent neurotoxicity through early endosome defects.

Acta neuropathologica communications

Lambert E,Saha O,Soares Landeira B,Melo de Farias AR,Hermant X,Carrier A,Pelletier A,Gadaut J,Davoine L,Dupont C,Amouyel P,Bonnefond A,Lafont F,Abdelfettah F,Verstreken P,Chapuis J,Barois N,Delahaye F,Dermaut B,Lambert JC,Costa MR,Dourlen P

14-9811-82 was used in Immunohistochemistry, Immunocytochemistry to demonstrate that the AD susceptibility gene BIN1, and especially BIN1iso1, contributes to early-endosome size deregulation, which is an early pathophysiological hallmark of AD pathology.

Sat Jan 08 00:00:00 EST 2022

The Alzheimer susceptibility gene BIN1 induces isoform-dependent neurotoxicity through early endosome defects.

Acta neuropathologica communications

Sat Jan 08 00:00:00 EST 2022

Modulating mesendoderm competence during human germ layer differentiation.

Cell reports

Valcourt JR,Huang RE,Kundu S,Venkatasubramanian D,Kingston RE,Ramanathan S

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunocytochemistry

Tue Nov 09 00:00:00 EST 2021

Modulating mesendoderm competence during human germ layer differentiation.

Cell reports

Valcourt JR,Huang RE,Kundu S,Venkatasubramanian D,Kingston RE,Ramanathan S

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunocytochemistry

Tue Nov 09 00:00:00 EST 2021

PD-1 independent of PD-L1 ligation promotes glioblastoma growth through the NFκB pathway.

Science advances

Mirzaei R,Gordon A,Zemp FJ,Kumar M,Sarkar S,Luchman HA,Bellail AC,Hao C,Mahoney DJ,Dunn JF,Bose P,Yong VW

14-9811-82 was used in Immunohistochemistry (Paraffin), Immunocytochemistry to point to a critical role for PD-1 in BTICs and uncover a nonimmune resistance mechanism of patients with glioblastoma to PD-1- or PD-L1-blocking therapies.

Fri Nov 05 00:00:00 EDT 2021

PD-1 independent of PD-L1 ligation promotes glioblastoma growth through the NFκB pathway.

Science advances

Mirzaei R,Gordon A,Zemp FJ,Kumar M,Sarkar S,Luchman HA,Bellail AC,Hao C,Mahoney DJ,Dunn JF,Bose P,Yong VW

Fri Nov 05 00:00:00 EDT 2021

Induction of inverted morphology in brain organoids by vertical-mixing bioreactors.

Communications biology

Suong DNA,Imamura K,Inoue I,Kabai R,Sakamoto S,Okumura T,Kato Y,Kondo T,Yada Y,Klein WL,Watanabe A,Inoue H

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunohistochemistry (Frozen)

Fri Oct 22 00:00:00 EDT 2021

Wnt/β-catenin signalling is dispensable for adult neural stem cell homeostasis and activation.

Development (Cambridge, England)

Austin SHL,Gabarró-Solanas R,Rigo P,Paun O,Harris L,Guillemot F,Urbán N

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunocytochemistry

Fri Oct 15 00:00:00 EDT 2021

Wnt/β-catenin signalling is dispensable for adult neural stem cell homeostasis and activation.

Development (Cambridge, England)

Austin SHL,Gabarró-Solanas R,Rigo P,Paun O,Harris L,Guillemot F,Urbán N

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunocytochemistry

Fri Oct 15 00:00:00 EDT 2021

Pharmacological targeting PTK6 inhibits the JAK2/STAT3 sustained stemness and reverses chemoresistance of colorectal cancer.

Journal of experimental & clinical cancer research : CR

Liu C,Pan Z,Chen Q,Chen Z,Liu W,Wu L,Jiang M,Lin W,Zhang Y,Lin W,Zhou R,Zhao L

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Flow Cytometry

Wed Sep 22 00:00:00 EDT 2021

Fibrinogen in the glioblastoma microenvironment contributes to the invasiveness of brain tumor-initiating cells.

Brain pathology (Zurich, Switzerland)

Dzikowski L,Mirzaei R,Sarkar S,Kumar M,Bose P,Bellail A,Hao C,Yong VW

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunocytochemistry

Wed Sep 01 00:00:00 EDT 2021

Fibrinogen in the glioblastoma microenvironment contributes to the invasiveness of brain tumor-initiating cells.

Brain pathology (Zurich, Switzerland)

Dzikowski L,Mirzaei R,Sarkar S,Kumar M,Bose P,Bellail A,Hao C,Yong VW

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunocytochemistry

Wed Sep 01 00:00:00 EDT 2021

Glial-Specific Deletion of Med12 Results in Rapid Hearing Loss via Degradation of the Stria Vascularis.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Huang TW,Iyer AA,Manalo JM,Woo J,Bosquez Huerta NA,McGovern MM,Schrewe H,Pereira FA,Groves AK,Ohlemiller KK,Deneen B

14-9811-82 was used in Immunohistochemistry (Frozen) to study the effects of temporal conditional deletion of Med12 in astrocytes in the adult CNS.

Wed Aug 25 00:00:00 EDT 2021

Quantitative analysis of signaling responses during mouse primordial germ cell specification.

Biology open

Morgani SM,Hadjantonakis AK

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunocytochemistry

Sat May 15 00:00:00 EDT 2021

Elp2 mutations perturb the epitranscriptome and lead to a complex neurodevelopmental phenotype.

Nature communications

Kojic M,Gawda T,Gaik M,Begg A,Salerno-Kochan A,Kurniawan ND,Jones A,Drożdżyk K,Kościelniak A,Chramiec-Głąbik A,Hediyeh-Zadeh S,Kasherman M,Shim WJ,Sinniah E,Genovesi LA,Abrahamsen RK,Fenger CD,Madsen CG,Cohen JS,Fatemi A,Stark Z,Lunke S,Lee J,Hansen JK,Boxill MF,Keren B,Marey I,Saenz MS,Brown K,Alexander SA,Mureev S,Batzilla A,Davis MJ,Piper M,Bodén M,Burne THJ,Palpant NJ,Møller RS,Glatt S,Wainwright BJ

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunohistochemistry

Tue May 11 00:00:00 EDT 2021

The transcription factor Rreb1 regulates epithelial architecture, invasiveness, and vasculogenesis in early mouse embryos.

eLife

Morgani SM,Su J,Nichols J,Massagué J,Hadjantonakis AK

14-9811 was used in Immunohistochemistry to determine that ras-responsive element-binding protein 1 is essential for mouse embryonic development, with a possible role in cancer and diseases involving loss of epithelial integrity.

Fri Apr 30 00:00:00 EDT 2021

Identification of Sox2 and NeuN Double-Positive Cells in the Mouse Hypothalamic Arcuate Nucleus and Their Reduction in Number With Aging.

Frontiers in aging neuroscience

Sugiura A,Shimizu T,Kameyama T,Maruo T,Kedashiro S,Miyata M,Mizutani K,Takai Y

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunocytochemistry

Tue Mar 30 00:00:00 EDT 2021

Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency.

Cell stem cell

Kinoshita M,Barber M,Mansfield W,Cui Y,Spindlow D,Stirparo GG,Dietmann S,Nichols J,Smith A

14-9811-82 was used in Immunocytochemistry to suggest that formative stem cells which respond directly to germ cell induction can be cultured from mice and humans.

Thu Mar 04 00:00:00 EST 2021

Cancer Stem Cells in Metastatic Head and Neck Cutaneous Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System.

Cells

Siljee S,Buchanan O,Brasch HD,Bockett N,Patel J,Paterson E,Purdie GL,Davis PF,Itinteang T,Tan ST

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunohistochemistry

Wed Jan 27 00:00:00 EST 2021

Cancer Stem Cells in Metastatic Head and Neck Cutaneous Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System.

Cells

Siljee S,Buchanan O,Brasch HD,Bockett N,Patel J,Paterson E,Purdie GL,Davis PF,Itinteang T,Tan ST

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunohistochemistry

Wed Jan 27 00:00:00 EST 2021

Altered cytoskeletal arrangement in induced pluripotent stem cells (iPSCs) and motor neurons from patients with riboflavin transporter deficiency.

Disease models & mechanisms

Niceforo A,Marioli C,Colasuonno F,Petrini S,Massey K,Tartaglia M,Bertini E,Moreno S,Compagnucci C

14-9811 was used in Immunocytochemistry-Immunofluorescence to show that RF administration resulted in partially improved cytoskeletal features in patients' iPSCs and MNs.

Tue Jan 19 00:00:00 EST 2021

Stromal SOX2 Upregulation Promotes Tumorigenesis through the Generation of a SFRP1/2-Expressing Cancer-Associated Fibroblast Population.

Developmental cell

Kasashima H,Duran A,Martinez-Ordoñez A,Nakanishi Y,Kinoshita H,Linares JF,Reina-Campos M,Kudo Y,L'Hermitte A,Yashiro M,Ohira M,Bao F,Tauriello DVF,Batlle E,Diaz-Meco MT,Moscat J

14-9811-82 was used in Immunohistochemistry to explore the transcriptional mechanisms regulating cancer-associated fibroblasts in promoting tumour malignancy.

Mon Jan 11 00:00:00 EST 2021

An epilepsy-associated ACTL6B variant captures neuronal hyperexcitability in a human induced pluripotent stem cell model.

Journal of neuroscience research

Ahn LY,Coatti GC,Liu J,Gumus E,Schaffer AE,Miranda HC

14-9811 was used in Immunocytochemistry to investigate the molecular pathogenesis of ACTL6B p.(Val421_Cys425del) using in silico 3D protein modeling predictions and patient-specific induced pluripotent stem cell-derived neurons.

Fri Jan 01 00:00:00 EST 2021

An epilepsy-associated ACTL6B variant captures neuronal hyperexcitability in a human induced pluripotent stem cell model.

Journal of neuroscience research

Ahn LY,Coatti GC,Liu J,Gumus E,Schaffer AE,Miranda HC

Fri Jan 01 00:00:00 EST 2021

The common marmoset as suitable nonhuman alternative for the analysis of primate cochlear development.

The FEBS journal

Hosoya M,Fujioka M,Murayama AY,Okano H,Ogawa K

14-9811 was used in Immunohistochemistry-immunofluorescence to support the use of the common marmoset as an effective alternative for analyses of primate cochlear development.

Fri Jan 01 00:00:00 EST 2021

Keratin 13 deficiency causes white sponge nevus in mice.

Developmental biology

Simonson L,Vold S,Mowers C,Massey RJ,Ong IM,Longley BJ,Chang H

14-9811-82 was used in Immunohistochemistry to determine whether there is a causal relationship between keratin 13 mutations and white sponge nevus and what the underlying mechanisms might be.

Tue Dec 01 00:00:00 EST 2020

Cancer Stem Cells in Head and Neck Metastatic Malignant Melanoma Express Components of the Renin-Angiotensin System.

Life (Basel, Switzerland)

Siljee S,Pilkington T,Brasch HD,Bockett N,Patel J,Paterson E,Davis PF,Tan ST

14-9811 was used in Immunohistochemistry-immunofluorescence to investigate expression of the RAS by the CSC subpopulations in human head and neck metastatic malignant melanoma (HNmMM) tissue samples and HNmMM-derived primary cell lines.

Mon Nov 02 00:00:00 EST 2020

Initiation of a conserved trophectoderm program in human, cow and mouse embryos.

Nature

Gerri C,McCarthy A,Alanis-Lobato G,Demtschenko A,Bruneau A,Loubersac S,Fogarty NME,Hampshire D,Elder K,Snell P,Christie L,David L,Van de Velde H,Fouladi-Nashta AA,Niakan KK

14-9811-82 was used in Immunocytochemistry to demonstrate that inhibition of aPKC by small-molecule pharmacological modulation or Trim-Away protein depletion impairs TE initiation at the morula stage.

Sun Nov 01 00:00:00 EDT 2020

Embryonic disc formation following post-hatching bovine embryo development in vitro.

Reproduction (Cambridge, England)

Ramos-Ibeas P,Lamas-Toranzo I,Martínez-Moro Á,de Frutos C,Quiroga AC,Zurita E,Bermejo-Álvarez P

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunocytochemistry

Thu Oct 01 00:00:00 EDT 2020

Embryonic disc formation following post-hatching bovine embryo development in vitro.

Reproduction (Cambridge, England)

Ramos-Ibeas P,Lamas-Toranzo I,Martínez-Moro Á,de Frutos C,Quiroga AC,Zurita E,Bermejo-Álvarez P

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunocytochemistry

Thu Oct 01 00:00:00 EDT 2020

Microfluidic platform for 3D cell culture with live imaging and clone retrieval.

Lab on a chip

Mulas C,Hodgson AC,Kohler TN,Agley CC,Humphreys P,Kleine-Brüggeney H,Hollfelder F,Smith A,Chalut KJ

14-9811-82 was used in Immunocytochemistry to present a highly flexible 3D cell encapsulation and microfluidic platform that enables both monitoring of cellular dynamics and retrieval for molecular and functional assays.

Tue Jul 14 00:00:00 EDT 2020

Differential regulation of lineage commitment in human and mouse primed pluripotent stem cells by the nucleosome remodelling and deacetylation complex.

Stem cell research

Ragheb R,Gharbi S,Cramard J,Ogundele O,Kloet SL,Burgold T,Vermeulen M,Reynolds N,Hendrich B

14-9811-82 was used in Immunocytochemistry to reveal that failure to properly fine-tune gene expression and/or to reduce transcriptional noise through the action of a highly conserved chromatin remodeller can have different consequences in human and mouse pluripotent stem cells.

Wed Jul 01 00:00:00 EDT 2020

Zika Virus with Increased CpG Dinucleotide Frequencies Shows Oncolytic Activity in Glioblastoma Stem Cells.

Viruses

Trus I,Berube N,Jiang P,Rak J,Gerdts V,Karniychuk U

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunocytochemistry

Mon May 25 00:00:00 EDT 2020

Zika Virus with Increased CpG Dinucleotide Frequencies Shows Oncolytic Activity in Glioblastoma Stem Cells.

Viruses

Trus I,Berube N,Jiang P,Rak J,Gerdts V,Karniychuk U

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunocytochemistry

Mon May 25 00:00:00 EDT 2020

Analysis of Dual Class I Histone Deacetylase and Lysine Demethylase Inhibitor Domatinostat (4SC-202) on Growth and Cellular and Genomic Landscape of Atypical Teratoid/Rhabdoid.

Cancers

Hoffman MM,Zylla JS,Bhattacharya S,Calar K,Hartman TW,Bhardwaj RD,Miskimins WK,de la Puente P,Gnimpieba EZ,Messerli SM

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Flow Cytometry

Mon Mar 23 00:00:00 EDT 2020

TGF-β orchestrates fibrogenic and developmental EMTs via the RAS effector RREB1.

Nature

Su J,Morgani SM,David CJ,Wang Q,Er EE,Huang YH,Basnet H,Zou Y,Shu W,Soni RK,Hendrickson RC,Hadjantonakis AK,Massagué J

14-9811 was used in Immunofluorescence on wholemounted tissues to reveal how TGF-β and RAS-MAPK signals acting jointly through SMAD and RREB1 transcription factors trigger EMTs in different contexts.

Wed Jan 01 00:00:00 EST 2020

Bcl2-Expressing Quiescent Type B Neural Stem Cells in the Ventricular-Subventricular Zone Are Resistant to Concurrent Temozolomide/X-Irradiation.

Stem cells (Dayton, Ohio)

Cameron BD,Traver G,Roland JT,Brockman AA,Dean D,Johnson L,Boyd K,Ihrie RA,Freeman ML

14-9811-82 was used in Immunohistochemistry (Paraffin) to demonstrate that murine type B neural stem cells residing in the ventricular-subventricular zone are tolerant of standard chemoradiation therapy, supporting a dose escalation strategy for treatment of very aggressive glioblastoma.

Sun Dec 01 00:00:00 EST 2019

3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling.

Cell stem cell

Zhang Y,Yang Y,Jiang M,Huang SX,Zhang W,Al Alam D,Danopoulos S,Mori M,Chen YW,Balasubramanian R,Chuva de Sousa Lopes SM,Serra C,Bialecka M,Kim E,Lin S,Toste de Carvalho ALR,Riccio PN,Cardoso WV,Zhang X,Snoeck HW,Que J

14-9811 was used in Immunocytochemistry-immunoflourescence to provide an efficient approach to generate EPCs, and offer a model system to study the regulatory mechanisms underlying development of the human esophagus.

Thu Oct 04 00:00:00 EDT 2018

BCL11A interacts with SOX2 to control the expression of epigenetic regulators in lung squamous carcinoma.

Nature communications

Lazarus KA,Hadi F,Zambon E,Bach K,Santolla MF,Watson JK,Correia LL,Das M,Ugur R,Pensa S,Becker L,Campos LS,Ladds G,Liu P,Evan GI,McCaughan FM,Le Quesne J,Lee JH,Calado D,Khaled WT

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunofluorescence

Mon Aug 20 00:00:00 EDT 2018

Spatial-Temporal Lineage Restrictions of Embryonic p63⁺ Progenitors Establish Distinct Stem Cell Pools in Adult Airways.

Developmental cell

Yang Y,Riccio P,Schotsaert M,Mori M,Lu J,Lee DK,García-Sastre A,Xu J,Cardoso WV

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunohistochemistry

Mon Mar 26 00:00:00 EDT 2018

Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning.

eLife

Morgani SM,Metzger JJ,Nichols J,Siggia ED,Hadjantonakis AK

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunofluorescence

Wed Feb 07 00:00:00 EST 2018

Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning.

eLife

Morgani SM,Metzger JJ,Nichols J,Siggia ED,Hadjantonakis AK

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunofluorescence

Wed Feb 07 00:00:00 EST 2018

Differential expression of microRNAs among cell populations in the regenerating adult mouse olfactory epithelium.

PloS one

Kurtenbach S,Ding W,Goss GM,Hare JM,Goldstein BJ,Shehadeh LA

14-9811 was used in Immunohistochemistry to demonstrate that microRNAs are differentially expressed by either progenitor or progeny cells in the regenerating mouse olfactory epithelium.

Tue Nov 28 00:00:00 EST 2017

Gelsolin dysfunction causes photoreceptor loss in induced pluripotent cell and animal retinitis pigmentosa models.

Nature communications

Megaw R,Abu-Arafeh H,Jungnickel M,Mellough C,Gurniak C,Witke W,Zhang W,Khanna H,Mill P,Dhillon B,Wright AF,Lako M,Ffrench-Constant C

14-9811 was used in Immunofluorescence to reveal a role for RPGR in gelosin activation, without which abnormalities in actin polymerisation in the photoreceptor connecting cilia cause rhodopsin mislocalisation and retinal degeneration in XLRP.

Wed Aug 16 00:00:00 EDT 2017

Dynamics of embryonic stem cell differentiation inferred from single-cell transcriptomics show a series of transitions through discrete cell states.

eLife

Jang S,Choubey S,Furchtgott L,Zou LN,Doyle A,Menon V,Loew EB,Krostag AR,Martinez RA,Madisen L,Levi BP,Ramanathan S

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Flow Cytometry

Wed Mar 15 00:00:00 EDT 2017

Dynamics of embryonic stem cell differentiation inferred from single-cell transcriptomics show a series of transitions through discrete cell states.

eLife

Jang S,Choubey S,Furchtgott L,Zou LN,Doyle A,Menon V,Loew EB,Krostag AR,Martinez RA,Madisen L,Levi BP,Ramanathan S

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Flow Cytometry

Wed Mar 15 00:00:00 EDT 2017

One-step generation of conditional and reversible gene knockouts.

Nature methods

Andersson-Rolf A,Mustata RC,Merenda A,Kim J,Perera S,Grego T,Andrews K,Tremble K,Silva JC,Fink J,Skarnes WC,Koo BK

14-9811 was used in Western Blotting and ICC/IF to present CRISPR-FLIP, a method providing efficient, rapid and scalable method for biallelic conditional gene knockouts.

Wed Mar 01 00:00:00 EST 2017

One-step generation of conditional and reversible gene knockouts.

Nature methods

Andersson-Rolf A,Mustata RC,Merenda A,Kim J,Perera S,Grego T,Andrews K,Tremble K,Silva JC,Fink J,Skarnes WC,Koo BK

14-9811 was used in Western Blotting and ICC/IF to present CRISPR-FLIP, a method providing efficient, rapid and scalable method for biallelic conditional gene knockouts.

Wed Mar 01 00:00:00 EST 2017

Selection and dynamics of embryonic stem cell integration into early mouse embryos.

Development (Cambridge, England)

Alexandrova S,Kalkan T,Humphreys P,Riddell A,Scognamiglio R,Trumpp A,Nichols J

14-9811 was used in Immunohistochemistry to examine the role of cellular heterogeneity in the incorporation of pluripotent cells into host embryos.

Fri Jan 01 00:00:00 EST 2016

Selection and dynamics of embryonic stem cell integration into early mouse embryos.

Development (Cambridge, England)

Alexandrova S,Kalkan T,Humphreys P,Riddell A,Scognamiglio R,Trumpp A,Nichols J

14-9811 was used in Immunohistochemistry to examine the role of cellular heterogeneity in the incorporation of pluripotent cells into host embryos.

Fri Jan 01 00:00:00 EST 2016

SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1).

Development (Cambridge, England)

Yamamizu K,Schlessinger D,Ko MS

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunofluorescence

Sat Nov 01 00:00:00 EDT 2014

SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1).

Development (Cambridge, England)

Yamamizu K,Schlessinger D,Ko MS

Published figure using SOX2 monoclonal antibody (Product # 14-9811-82) in Immunofluorescence

Sat Nov 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); sex determining region Y-box 2; SOX 2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY related HMG box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2

Gene Aliases: ANOP3; lcc; MCOPS3; Sox-2; SOX2; ysb

UniProt ID: (Human) P48431, (Mouse) P48432

Entrez Gene ID: (Human) 6657, (Mouse) 20674

Molecular Function: HMG box transcription factor