Invitrogen

SOX2 Monoclonal Antibody (Btjce), eFluor™ 660, eBioscience™

Product Image Gallery

SOX2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry on fixed and permeabilized induced pluripotent stem cells using 10 µg/mL Anti-Human/Mouse Sox2 eFluor® 660 (right, image courtesy of Dr. A. Firth, Salk Institute).

SOX2 Antibody in Flow Cytometry (Flow) — Intracellular staining of untreated (pink) and retinoic acid treated (yellow) F9 cell line with 0.125 µg of Rat IgG2a kappa Isotype Control eFluor® 660 (left) or 0.125 µg of Anti-Human/Mouse Sox2 eFluor® 660 (right) using Foxp3 Staining Buffers. Total cells were used for analysis.

SOX2 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Fig. 2. NSCs and adult hippocampal neurogenesis are unaffected by the NSC-specific deletion of beta-catenin and inhibition of Wnt/beta-catenin signalling. (A) Two-month-old control and beta-cat del ex2-6 mice were administered tamoxifen for 5 consecutive days and sacrificed 30 days after the first tamoxifen injection. (B) YFP, GFAP, SOX2 and Ki67 immunolabelling in the DG of control and beta-cat del ex2-6 mice 30 days after tamoxifen administration. White-dashed lines denote the SGZ. (C-F) Quantification of the data shown in B. C: proportion of Ki67+ NSCs (control versus beta-cat del ex2-6 : 3.33+-0.61% versus 3.333+-0.76%). D: total number of NSCs (YFP+ GFAP+ SOX2+ radial cells in the SGZ) normalised to the length of the SGZ (mm) (control versus beta-cat del ex2-6 : 21.2+-1.73 versus 23.56+-1.52). E: proportion of proliferating (Ki67+) IPCs (SOX2+ YFP+ GFAP- cells in the SGZ; control versus beta-cat del ex2-6 : 56+-4.56% versus 47.67+-4.03%). F: total number of IPCs normalised to the length of the SGZ (mm) (SOX2+ YFP+ GFAP- cells in the SGZ; control versus beta-cat del ex2-6 : 20.18+-2.31 versus 22.43+-2.62). n =6. (G) YFP, TBR2 and DCX immunolabelling in the DG of control and beta-cat del ex2-6 mice 30 days after tamoxifen administration. (H,I) Quantification of the data shown in G. H: total number of TBR2+ IPCs normalised to the SGZ length (mm) (TBR2+ YFP+ cells in the SGZ; control versus beta-cat del ex2-6 : 14.29+-1.51 versus 17.16+-3.09). I: total number of neuroblasts

SOX2 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Brain organoid generated by vertical mixing showed inverted structure in comparison with brain organoid generated by orbital mixing. a Schematic diagram of conditions used to induce brain organoid by orbital mixing (upper schema) and by vertical mixing (lower schema). b , c Immunostaining for neural progenitor (SOX2, magenta) and neuron (MAP2, green) in brain organoid generated by orbital mixing ( b ) or vertical mixing ( c ) on Day 56. d Quantification of SOX2-positive area in the peripheral region of brain organoid on Day 56. The peripheral region was defined as 100 um inside from the edge of brain organoid. Brain organoid from vertical mixing showed higher percentage of SOX2-positive area in peripheral region in comparison with brain organoid from orbital mixing. Data represent mean +- SD ( n = 6 for brain organoids by orbital mixing, n = 7 for brain organoids by vertical mixing). Difference between the two conditions was analyzed by Student's two-tailed t -test (* p < 0.0001). e , f Immunostaining for markers of neural progenitors (SOX2, blue), intermediate neural progenitors (TBR2, gray), and cortical neurons (CTIP2, red; MAP2, green) in brain organoids by orbital mixing ( e ) or vertical mixing ( f ) on Day 56. g , h Immunostaining for markers of ventricular neuroepithelial cells (N-CADHERIN: N-CAD, gray), neural progenitors (SOX2, magenta), and cortical neurons (MAP2, green) in brain organoids by orbital mixing ( g ) or vertical mixing ( h ) on Day 56. Note the apical

SOX2 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Compartmental cell identity is altered upon Hedgehog pathway inhibition. RT-qPCR for SOX2 ( A ), SOX9 ( B ), and ACTA2 ( C ) in fetal lung explants treated with 5E1 compared to control. Results are shown as mean +- SEM, * p < 0.05, n = 6 for each group. ( D - H ) Western blot analysis of protein expression of SOX2 ( D , E ), SOX9 ( F , G ), and ACTA2 ( H , I ) in 5E1 treated explants compared to control ( n = 3 for each condition). Western blot densitometry ratios are shown in ( E , G , I ) ( n = 3 for each condition). Results are shown as mean +- SEM. IF staining of fetal lung explants treated ( K ) or not ( J ) with 5E1 for SOX2 (green), SOX9 (white), and ACTA2 (red). Quantification of the number of epithelial positive SOX2 cells ( L ) and SOX9 ( M ) ( n = 4 for each group). * p < 0.05, ** p < 0.01; ns = no stress.

SOX2 Antibody in Immunohistochemistry (IHC) — Fig. 7. PD-1 on BTICs promotes intracranial tumor growth in mice. ( A ) Schematic diagram depicts implantation of Pdcd1 -knockdown or OE cells into the striatum of mice. Kaplan-Meier analysis to assess the survival of wild-type mice i4mplanted with ( B ) Pdcd1 knockdown or ( C ) overexpressing murine BT0309. Cells transduced with lentiviral vectors coding nontarget shRNA or transfected with empty PiggyBac vector were considered as controls for knockdown (shRNA control) or OE (OE control) BTICs, respectively. ( D ) Representative images of immunofluorescence staining of PD-1, SOX2, and p65 in sections collected from C57BL/6 wild-type mice implanted with Pdcd1 -knockdown or shRNA controls. Nuclear translocation (yellow arrowheads) and cytoplasmic localization (blue arrowheads) of p65 in BTICs are shown on the images. ( E ) Kaplan-Meier analysis of Rag1 KO mice implanted with Pdcd1 -knockdown or shRNA controls of mBT0309. Representative T2-weighted MRI images ( F ) at day 50 of Rag1 KO mice implanted with Pdcd1 -knockdown or ( G ) at day 54 of Rag1 KO mice implanted with Pdcd1 OE mBT0309 (tumor demarcated with red outlines). Quantitation of tumor volume from MRI images of mice implanted with ( H ) Pdcd1 knockdown or ( I ) OE BTICs versus respective vector controls. Results are representative of n = 2 to 3 independent experiments. Unpaired (two-tailed) t test was used to assess significance between two groups in (I). For more than two groups in (H), one-way ANOVA with Tukey's pos

Product Details

50-9811-82

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 8 publications 8 publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	Assay-Dependent	View 11 publications 11 publications
Flow Cytometry (Flow)	0.125 µg/test	View 4 publications 4 publications

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	Btjce
Conjugate	eFluor™ 660 Alexa Fluor 488 eFluor 570 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_11220483

Product Specific Information

Description: The Btjce monoclonal antibody reacts with the transcription factor Sox2, a member of the SOX (sex determining region Y -related HMG (High Mobility Group) Box) family of proteins. Sox family members play a role in early organ development, and in particular, Sox2 is essential for regulating genes that control normal mammalian embryogenesis. Sox2 and family member Sox3 are expressed as early as the preimplantation and epiblast stages respectively. Later expression is restricted to the neuroepithelium. Sox2 has been shown to be necessary for maintaining self-renewal and pluripotency of mouse and human embryonic stem (ES) cells (ESC). Oct4 (POU5F1), Klf4, c-myc, and Sox2 were the original four factors used to reprogram differentiated mouse and human cells to induced pluripotent stem cells (iPSC).

Expression of Sox2 is tightly regulated and recent studies have demonstrated that small changes in the levels of Sox2 in ES cells can trigger differentiation into multiple cell types. Sox2 expression is not limited to ES cells, it is also essential for early neurogenesis where its expression becomes restricted to the neural plate, and later to neural stem cells where it functions to suppress neural differentiation. Sox2 in combination with other stem cell markers can be used to characterize stem cell populations. Ectopic expression of Sox2 has been associated with multiple cancer types including colorectal and breast.

Applications Reported: This btjce antibody has been reported for use in intracellular staining followed by flow cytometric analysis and immunocytochemistry.

Applications Tested: This btjce antibody has been tested by immunocyochemstry of fixed and permeabilized F9 cells. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

This btjce has also been tested by intracellular staining and flow cytometric analysis using the Foxp3/Transcription Factor Buffer and protocol. Please refer to intracellular Staining Protocol on Best Protocol web page - Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Bacterial expression of region after aa135 (homology between species but not to other family members)

Target Information

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Hedgehog Signaling Pathway Orchestrates Human Lung Branching Morphogenesis.

International journal of molecular sciences

Belgacemi R,Danopoulos S,Deutsch G,Glass I,Dormoy V,Bellusci S,Al Alam D

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunohistochemistry

Mon May 09 00:00:00 EDT 2022

Hedgehog Signaling Pathway Orchestrates Human Lung Branching Morphogenesis.

International journal of molecular sciences

Belgacemi R,Danopoulos S,Deutsch G,Glass I,Dormoy V,Bellusci S,Al Alam D

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunohistochemistry

Mon May 09 00:00:00 EDT 2022

Modulating mesendoderm competence during human germ layer differentiation.

Cell reports

Valcourt JR,Huang RE,Kundu S,Venkatasubramanian D,Kingston RE,Ramanathan S

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunocytochemistry

Tue Nov 09 00:00:00 EST 2021

PD-1 independent of PD-L1 ligation promotes glioblastoma growth through the NFκB pathway.

Science advances

Mirzaei R,Gordon A,Zemp FJ,Kumar M,Sarkar S,Luchman HA,Bellail AC,Hao C,Mahoney DJ,Dunn JF,Bose P,Yong VW

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunocytochemistry

Fri Nov 05 00:00:00 EDT 2021

Induction of inverted morphology in brain organoids by vertical-mixing bioreactors.

Communications biology

Suong DNA,Imamura K,Inoue I,Kabai R,Sakamoto S,Okumura T,Kato Y,Kondo T,Yada Y,Klein WL,Watanabe A,Inoue H

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunohistochemistry (Frozen)

Fri Oct 22 00:00:00 EDT 2021

Wnt/β-catenin signalling is dispensable for adult neural stem cell homeostasis and activation.

Development (Cambridge, England)

Austin SHL,Gabarró-Solanas R,Rigo P,Paun O,Harris L,Guillemot F,Urbán N

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunocytochemistry

Fri Oct 15 00:00:00 EDT 2021

Wnt/β-catenin signalling is dispensable for adult neural stem cell homeostasis and activation.

Development (Cambridge, England)

Austin SHL,Gabarró-Solanas R,Rigo P,Paun O,Harris L,Guillemot F,Urbán N

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunocytochemistry

Fri Oct 15 00:00:00 EDT 2021

Pharmacological targeting PTK6 inhibits the JAK2/STAT3 sustained stemness and reverses chemoresistance of colorectal cancer.

Journal of experimental & clinical cancer research : CR

Liu C,Pan Z,Chen Q,Chen Z,Liu W,Wu L,Jiang M,Lin W,Zhang Y,Lin W,Zhou R,Zhao L

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Flow Cytometry

Wed Sep 22 00:00:00 EDT 2021

Fibrinogen in the glioblastoma microenvironment contributes to the invasiveness of brain tumor-initiating cells.

Brain pathology (Zurich, Switzerland)

Dzikowski L,Mirzaei R,Sarkar S,Kumar M,Bose P,Bellail A,Hao C,Yong VW

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunocytochemistry

Wed Sep 01 00:00:00 EDT 2021

Quantitative analysis of signaling responses during mouse primordial germ cell specification.

Biology open

Morgani SM,Hadjantonakis AK

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunocytochemistry

Sat May 15 00:00:00 EDT 2021

Elp2 mutations perturb the epitranscriptome and lead to a complex neurodevelopmental phenotype.

Nature communications

Kojic M,Gawda T,Gaik M,Begg A,Salerno-Kochan A,Kurniawan ND,Jones A,Drożdżyk K,Kościelniak A,Chramiec-Głąbik A,Hediyeh-Zadeh S,Kasherman M,Shim WJ,Sinniah E,Genovesi LA,Abrahamsen RK,Fenger CD,Madsen CG,Cohen JS,Fatemi A,Stark Z,Lunke S,Lee J,Hansen JK,Boxill MF,Keren B,Marey I,Saenz MS,Brown K,Alexander SA,Mureev S,Batzilla A,Davis MJ,Piper M,Bodén M,Burne THJ,Palpant NJ,Møller RS,Glatt S,Wainwright BJ

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunohistochemistry

Tue May 11 00:00:00 EDT 2021

Identification of Sox2 and NeuN Double-Positive Cells in the Mouse Hypothalamic Arcuate Nucleus and Their Reduction in Number With Aging.

Frontiers in aging neuroscience

Sugiura A,Shimizu T,Kameyama T,Maruo T,Kedashiro S,Miyata M,Mizutani K,Takai Y

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunocytochemistry

Tue Mar 30 00:00:00 EDT 2021

Cancer Stem Cells in Metastatic Head and Neck Cutaneous Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System.

Cells

Siljee S,Buchanan O,Brasch HD,Bockett N,Patel J,Paterson E,Purdie GL,Davis PF,Itinteang T,Tan ST

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunohistochemistry

Wed Jan 27 00:00:00 EST 2021

Embryonic disc formation following post-hatching bovine embryo development in vitro.

Reproduction (Cambridge, England)

Ramos-Ibeas P,Lamas-Toranzo I,Martínez-Moro Á,de Frutos C,Quiroga AC,Zurita E,Bermejo-Álvarez P

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunocytochemistry

Thu Oct 01 00:00:00 EDT 2020

Zika Virus with Increased CpG Dinucleotide Frequencies Shows Oncolytic Activity in Glioblastoma Stem Cells.

Viruses

Trus I,Berube N,Jiang P,Rak J,Gerdts V,Karniychuk U

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunocytochemistry

Mon May 25 00:00:00 EDT 2020

Analysis of Dual Class I Histone Deacetylase and Lysine Demethylase Inhibitor Domatinostat (4SC-202) on Growth and Cellular and Genomic Landscape of Atypical Teratoid/Rhabdoid.

Cancers

Hoffman MM,Zylla JS,Bhattacharya S,Calar K,Hartman TW,Bhardwaj RD,Miskimins WK,de la Puente P,Gnimpieba EZ,Messerli SM

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Flow Cytometry

Mon Mar 23 00:00:00 EDT 2020

Analysis of Dual Class I Histone Deacetylase and Lysine Demethylase Inhibitor Domatinostat (4SC-202) on Growth and Cellular and Genomic Landscape of Atypical Teratoid/Rhabdoid.

Cancers

Hoffman MM,Zylla JS,Bhattacharya S,Calar K,Hartman TW,Bhardwaj RD,Miskimins WK,de la Puente P,Gnimpieba EZ,Messerli SM

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Flow Cytometry

Mon Mar 23 00:00:00 EDT 2020

BCL11A interacts with SOX2 to control the expression of epigenetic regulators in lung squamous carcinoma.

Nature communications

Lazarus KA,Hadi F,Zambon E,Bach K,Santolla MF,Watson JK,Correia LL,Das M,Ugur R,Pensa S,Becker L,Campos LS,Ladds G,Liu P,Evan GI,McCaughan FM,Le Quesne J,Lee JH,Calado D,Khaled WT

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunofluorescence

Mon Aug 20 00:00:00 EDT 2018

Spatial-Temporal Lineage Restrictions of Embryonic p63⁺ Progenitors Establish Distinct Stem Cell Pools in Adult Airways.

Developmental cell

Yang Y,Riccio P,Schotsaert M,Mori M,Lu J,Lee DK,García-Sastre A,Xu J,Cardoso WV

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunohistochemistry

Mon Mar 26 00:00:00 EDT 2018

Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning.

eLife

Morgani SM,Metzger JJ,Nichols J,Siggia ED,Hadjantonakis AK

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunofluorescence

Wed Feb 07 00:00:00 EST 2018

Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning.

eLife

Morgani SM,Metzger JJ,Nichols J,Siggia ED,Hadjantonakis AK

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunofluorescence

Wed Feb 07 00:00:00 EST 2018

Dynamics of embryonic stem cell differentiation inferred from single-cell transcriptomics show a series of transitions through discrete cell states.

eLife

Jang S,Choubey S,Furchtgott L,Zou LN,Doyle A,Menon V,Loew EB,Krostag AR,Martinez RA,Madisen L,Levi BP,Ramanathan S

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Flow Cytometry

Wed Mar 15 00:00:00 EDT 2017

Selection and dynamics of embryonic stem cell integration into early mouse embryos.

Development (Cambridge, England)

Alexandrova S,Kalkan T,Humphreys P,Riddell A,Scognamiglio R,Trumpp A,Nichols J

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunofluorescence

Fri Jan 01 00:00:00 EST 2016

Selection and dynamics of embryonic stem cell integration into early mouse embryos.

Development (Cambridge, England)

Alexandrova S,Kalkan T,Humphreys P,Riddell A,Scognamiglio R,Trumpp A,Nichols J

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunofluorescence

Fri Jan 01 00:00:00 EST 2016

Activated CD8+ T lymphocytes inhibit neural stem/progenitor cell proliferation: role of interferon-gamma.

PloS one

Hu S,Rotschafer JH,Lokensgard JR,Cheeran MC

50-9811 was used in Flow cytometry/Cell sorting to investigate the effect of chronic neuroinflammation on neural stem cells (NSCs) and subsequent therapeutic implications for NSC therapies in neurodegenerative disorders.

Thu Apr 23 00:00:00 EDT 2015

SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1).

Development (Cambridge, England)

Yamamizu K,Schlessinger D,Ko MS

Published figure using SOX2 monoclonal antibody (Product # 50-9811-82) in Immunofluorescence

Sat Nov 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); sex determining region Y-box 2; SOX 2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY related HMG box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2

Gene Aliases: ANOP3; lcc; MCOPS3; Sox-2; SOX2; ysb

UniProt ID: (Human) P48431, (Mouse) P48432

Entrez Gene ID: (Human) 6657, (Mouse) 20674

Molecular Function: HMG box transcription factor