Invitrogen

SOX2 Monoclonal Antibody (Btjce), eFluor 660, eBioscience™

Product Image Gallery

SOX2 Antibody — Fig. 2 BCL11A overexpression leads to thickening of the airways and abnormal organoid formation. a Schematic representing strategy to explore the role of BCL11A in vivo and ex vivo. Left Panel: Adenovirus-Cre was nasally administered to BCL11A ovx mice and the lungs were analysed after eight months. Right panel: for the tracheosphere organoid model, basal cells from the trachea of either BCL11A ovx or BCL11A cko mice were FACS sorted, embedded in matrigel and analysed after 15 days. Three independent mice were used for each experiment. b Images of airways from control and BCL11A ovx . Arrows indicate small hyperchromatic cells with irregular nuclei. c Quantificaiton of airway epithelial layer hyperplasia from two control and BCL11A ovx mice. d Bright field images of organoids from Bcl11a cko and BCL11A ovx mice treated with vehicle or tamoxifen. e Sectioned organoids from BCL11A ovx mice stained with haematoxylin and eosin, BCL11A, Sox2, Krt5, Trp63 and Krt8. Arrows indicate positive staining. Scale bar indicates 50 mum

SOX2 Antibody in Flow Cytometry (Flow) — Intracellular staining of untreated (pink) and retinoic acid treated (yellow) F9 cell line with 0.125 µg of Rat IgG2a kappa Isotype Control eFluor® 660 (left) or 0.125 µg of Anti-Human/Mouse Sox2 eFluor® 660 (right) using Foxp3 Staining Buffers. Total cells were used for analysis.

SOX2 Antibody in Immunocytochemistry (ICC) — Immunocytochemistry on fixed and permeabilized induced pluripotent stem cells using 10 µg/mL Anti-Human/Mouse Sox2 eFluor® 660 (right, image courtesy of Dr. A. Firth, Salk Institute).

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.125 µg/test

Immunocytochemistry (ICC)

Assay-Dependent

Immunofluorescence (IF)

Assay-Dependent

Published Applications

Immunofluorescence (IF)

See 4 publications below

Flow Cytometry (Flow)

See 2 publications below

Immunohistochemistry (IHC)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host / Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Btjce

Conjugate

eFluor® 660

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_11220483

Product Specific Information

Description: The Btjce monoclonal antibody reacts with the transcription factor Sox2, a member of the SOX (sex determining region Y -related HMG (High Mobility Group) Box) family of proteins. Sox family members play a role in early organ development, and in particular, Sox2 is essential for regulating genes that control normal mammalian embryogenesis. Sox2 and family member Sox3 are expressed as early as the preimplantation and epiblast stages respectively. Later expression is restricted to the neuroepithelium. Sox2 has been shown to be necessary for maintaining self-renewal and pluripotency of mouse and human embryonic stem (ES) cells (ESC). Oct4 (POU5F1), Klf4, c-myc, and Sox2 were the original four factors used to reprogram differentiated mouse and human cells to induced pluripotent stem cells (iPSC).

Expression of Sox2 is tightly regulated and recent studies have demonstrated that small changes in the levels of Sox2 in ES cells can trigger differentiation into multiple cell types. Sox2 expression is not limited to ES cells, it is also essential for early neurogenesis where its expression becomes restricted to the neural plate, and later to neural stem cells where it functions to suppress neural differentiation. Sox2 in combination with other stem cell markers can be used to characterize stem cell populations. Ectopic expression of Sox2 has been associated with multiple cancer types including colorectal and breast.

Applications Reported: This btjce antibody has been reported for use in intracellular staining followed by flow cytometric analysis and immunocytochemistry.

Applications Tested: This btjce antibody has been tested by immunocyochemstry of fixed and permeabilized F9 cells. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

This btjce has also been tested by intracellular staining and flow cytometric analysis using the Foxp3/Transcription Factor Buffer and protocol. Please refer to intracellular Staining Protocol on Best Protocol web page - Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ANOP3; MCOPS3; sex determining region Y box 2; sex determining region Y-box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2

Gene Aliases: ANOP3; lcc; MCOPS3; Sox-2; SOX2; ysb

UniProt ID: (Human) P48431, (Mouse) P48432

Entrez Gene ID: (Human) 6657, (Mouse) 20674

Molecular Function: HMG box transcription factor nucleic acid binding transcription factor