Description: The monoclonal antibody eBioP4D1 (p4D1) recognizes ubiquitin, a small 8Ka protein. Ubiquitin units are linked to proteins via a ubiquitination process, thereby signaling the protein for degradation by the 26S proteosome. The process of ubiquitination plays a role in differentiation, cell cycle progression, apoptosis and disease progression. For example, neuronal inclusions called Lewis bodies from Parkinson patients are rich in ubiquitin.
Applications Reported: This eBioP4D1 (P4D1) antibody has been reported for use in immunoblotting (WB).
Applications Tested: This eBioP4D1 (P4D1) antibody has been tested by immunoblotting of treated cell lines. This can be used at less than or equal to 0.5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Ubiquitin is a conserved 76 amino acid polypeptide and can affect proteasomal degradation of the protein it is bound to, or mediate interactions with other proteins related to post-translational modifications. The degradation of cellular regulatory proteins by the Uubiquitin pathway is important as it controls the cellular growth and proliferation. Ubiquitin-dependent proteolysis occurs after a covalent attachment of the peptide to a lysine residue of a protein, which involves three enzymatic reactions: E1, E2 and E3. The first reaction involves ubiquitin-activating enzyme. The third reaction uses enzyme ubiquitin ligase (E3) to transfer the activated ubiquitin from E2 to a lysine residue on a protein, or directly transfers the ubiquitin from E2 to the substrate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 40S ribosomal protein S27a; epididymis luminal protein 112; HUBCEP52; RPL40; Small ribosomal subunit protein eS31; Ubiquiti; Ubiquitin; Ubiquitin 52 amino acid fusion protein; Ubiquitin A52 residue ribosomal protein fusion product 1; ubiquitin and ribosomal protein S27a; ubiquitin C; Ubiquitin carboxyl extension protein 80; Ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a-like; ubiquitin-CEP80
Gene Aliases: 0610006J14Rik; CEP80; HEL112; RPS27A; S27A; Uba52; UBA80; Ubb; UBC; UBCEP1; UBCEP80