Gibco™ CHO CD EfficientFeed™ A nutrient supplement is an animal origin-free, chemically defined complete feed formulation that provides up to 2-5 fold improvement in titers used in fed batch versus batch culture. It contains no proteins, hydrolysates, or components of incompletely defined composition. CHO CD EfficientFeed™ A AGT™ (Advanced Granulation Technology™) Kit Nutrient Supplement provides all the benefits of CHO CD EfficientFeed™ A liquid supplement in a simpler to scale up and reconstitute 2-part kit that is primarily AGT™ dry format material.
The AGT™ format is designed for large scale manufacturing and ease of use. This product is also available in
liquid form for small scale use and experimentation.
• Expect up to 2- to 5-fold increases in productivity
• Avoid issues associated with the use of hydrolysates and other alternatives while gaining superior performance
• Simplify scale-up by leveraging the value of AGT™ simple reconstitution
• Achieve performance comparable to original liquid supplement
• Ready for larger scale use with convenient packaging options (10 L and 100 L)
Which EfficientFeed™ Nutrient Supplement to Choose?Consider the
CHO CD Efficient Feed™ Kit when:
• You are using a base medium with less than 6 g/L glucose and no hydrolysate at day 0
Consider CHO
CD EfficientFeed™ A supplement when:
• You are currently using CD OptiCHO™ as your base cell growth medium
Consider CHO
CD EfficientFeed™ B supplement when:
• You are currently using CD CHO as your base cell growth medium
Consider
CD EfficientFeed™ C AGT™ supplement when:
• You want higher productivity in GS CHO and other selected cell lines
• You are currently using CD FortiCHO™ as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at day 0
Increased TitersCHO CD EfficientFeed™ supplements have been shown to increase titers 2-5 fold versus batch culture, and are an excellent starting point for process development (Figures 1a & 1b). Furthermore, they outperform competitive alternatives, including less consistent hydrolysates (Figures 2, 3a, & 3b).
Simple Scale-Up and Cost SavingsThe ability to scale up in a dry format provides ease of handling and storage in manufacturing and other bioprocess settings. With AGT™ (Advanced Granulation Technology™) dry format, it is possible to store and use the product even more easily and safely than traditional dry format products. CHO CD EfficientFeed™ A AGT™ supplement is offered in 10 L and 100 L packaging that allows scale-up from the 1 L liquid supplement as a seamless process, as well as allow for cost savings when purchasing production level volumes.
Comparable Performance to Liquid SupplementEqually important as the convenience and simplicity of
AGT™ (Advanced Granulation Technology™) dry format is the comparable performance to the liquid
CHO CD EfficientFeed™ A version (Figure 4). Both forms have performed similarly in our testing, and the AGT™ version has been used successfully as a prototype in many processes by multiple companies.
AGT™ Format Ease of UseCHO CD EfficientFeed™ A AGT™ Kit Nutrient Supplement delivers dry format scale-up convenience as well as simple reconstitution with approximately 1% concentrated solution add back. For more details, consult the supporting documents provided below.
For research or further manufacturing use. Not for use in diagnostic procedures.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.