The pAd⁄CMV⁄V5-DEST™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ adenoviral expression vector, pAd⁄CMV⁄V5-DEST™ vector for easy recombination-based cloning and adenoviral-based,Read more
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Catalog number V49320
also known as V493-20
Price (USD)/ 6 µg
1,543.65
Online exclusive
1578.00
Save 34.35 (2%)
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Add to cart
Price (USD)/ 6 µg
1,543.65
Online exclusive
1578.00
Save 34.35 (2%)
Add to cart
pAd/CMV/V5-DEST™ Gateway™ Vector Kit
Catalog numberV49320
Price (USD)/ 6 µg
1,543.65
Online exclusive
1578.00
Save 34.35 (2%)
-
Add to cart
The pAd⁄CMV⁄V5-DEST™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ adenoviral expression vector, pAd⁄CMV⁄V5-DEST™ vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where constitutive, high-level expression is driven by the CMV promoter.
Advantages • High efficiency and rapid recombination cloning • Produces high titer adenoviral stocks • Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo • Produces replication-incompetent virus for enhanced biosafety of the system • Amenable for use in high-throughput applications
Key Features • Gateway™ Technology for efficient and rapid cloning • CMV promoter for high-level constitutive expression of gene of interest • Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions • Herpes Simplex Virus thymidine kinase (TK) polyadenylation sequence for efficient transcription termination and polyadenylation of mRNA • V5 epitope for detection of recombinant protein • Ampicillin selection marker
Kit Includes • pAd⁄CMV⁄V5-DEST™ Gateway™ Vector • pAd⁄CMV⁄V5-GW⁄lacZ control plasmid
For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible SystemConstitutive
Delivery TypeAdenoviral
PromoterCMV
Product TypeMammalian Expression Vector
Protein TagV5 Epitope Tag
Cloning MethodGateway
Quantity6 μg
VectorpDEST
Product LineGateway™, ViraPower™
For Use With (Application)Viral Expression
Unit Size6 µg
Contents & Storage
• pAd-CMV⁄V5-DEST™ Vector 150 ng⁄μl in TEBuffer, pH 8.0. 40 μl (Store at -20°C) • pAd⁄CMV⁄V5-GW⁄lacZ control plasmid 1 μg⁄μl in TE Buffer, pH 8.0. 10 μl (Store at -20°C)