Invitrogen™

eBioscience™ TMB Solution (1X)

Catalog number:  00-4201-56

Related applications:

Protein Assays and Analysis

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Description

The 1x eBioscience TMB Solution is a sensitive chromogenic substrate supplied as single ready-to-use solution. TMB (3,3’,5,5’-Tetramethylbenzidine) is a substrate for horseradish peroxidase (HRP) and is recommended for Ready-SET-Go! ELISA kits.

During the enzymatic reaction the TMB substrate will result in a blue color that can be read on a spectrophotometer at 650 nm. This kinetic reaction can be stopped using 1M phosphoric acid resulting in a color change to yellow that can be read at 450 nm.

Reported Application
ELISA

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Colorimetric
Product Line: eBioscience™
Product Size: 100 mL
Substrate Properties: Chemical Substrate
Substrate Type: HRP (Horseradish Peroxidase) Substrate