Attune™ NxT No-Wash No-Lyse Filter Kit

Catalog number:  100022776

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The Attune® NxT No-Wash No-Lyse Filter Kit enables configuration of the Attune® NxT Acoustic Focusing Cytometer with an additional side scatter channel off of the violet laser, allowing no-lyse no-wash immunophenotyping of whole blood. The Attune® NxT cytometer is quickly and easily configured to collect violet side scatter by changing dichroic and emission blades.

Enables no-wash/no-lyse application
Immunophenotyping of whole blood using traditional flow cytometry is typically performed with lysis of the red blood cells (RBCs) followed by a wash step. These steps can result in significant cell loss and damage.

While a no-lyse, no-wash method for immunophenotyping of whole blood has been previously described, the method is of limited utility in traditional flow cytometry, which use hydrodynamic forces to orient cells in the flow stream. Moreover, significant sample dilution is required in no-lyse, no-wash methods to achieve low coincidence with red blood cells, necessitating extremely long acquisition times.

With the Attune® NxT cytometer you can acquire data quickly, even from dilute samples, using either a fluorescent reagent as a trigger to eliminate red blood cells or a differential side scatter using both blue and violet scatter. This kit enables the side scatter approach.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Attune® Acoustic Focusing Cytometer
Product Line: Attune™
Product Size: 1 kit
Type: Filter

Contents & storage

Contains:
• 1 Dichroic 415LP filter
• 1 OD2 filter
• 1 Emission 405/10BP filter

Store at room temperature.

Documents

Manuals & protocols