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KDR (VEGFR2) Recombinant Human Protein, His Tag

Catalog number: 10012H08H25

 Related applications: Protein Assays and Analysis

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Description

Kinase insert domain receptor (KDR), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human KDR (NP_002244.1) (Met 1-Glu 764) fused with a polyhistidine tag at the C-terminus.

Activity: Using the Octet RED System. The affinity constant (Kd) of human KDR-His bound to biotinylated human NRP2 (VEGF165) was 5.9 nM.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Angiogenesis, Signal Transduction, Virology
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: CD309, FLK1, VEGFR, VEGFR2, KDR
Gene Symbol: KDR
Molecular Weight: 84.6 kDa
Product Line: Sino Biological®
Product Size: 5 x 5 µg
Protein Family: CD Proteins, Growth Factors & Receptors, Kinases & Inhibitors
Protein Form: Cell Surface Protein, Recombinant, Truncated
Protein Name: kinase insert domain receptor (a type III receptor tyrosine kinase)
Protein Subtype: VEGF (Vascular Endothelial Growth Factor)
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols

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