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MCAM Recombinant Human Protein, His Tag

Catalog number: 10115H08H5

 Related applications: Protein Assays and Analysis

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Description

Melanoma Cell Adhesion Molecule (MCAM), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human MCAM precursor (NP_006491.2) (Met 1-Gly 559) fused to a C-terminal polyhistidine tag.

Activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line. When 3 x 104 cells/well are added to human MCAM coated plates (0.8 µg/ml, 100 µl/well), approximately >50 % will adhere after one hour at 37°C.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: CD Proteins
Research Category: Angiogenesis, Immunology, Oncology
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: MCAM, CD146, MUC18
Gene Symbol: MCAM
Molecular Weight: 61.3 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Name: melanoma cell adhesion molecule
Protein Subtype: Intercellular adhesion Molecule
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols