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DKK1 Recombinant Human Protein, His Tag

Catalog number: 10170H08H5

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Description

Dickkopf 1 homolog (Xenopus laevis) (DKK1), recombinant rhesus protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the human DKK1 precursor (NP_036374.1) (Met 2-His 266) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its ability to inhibit WNT3A-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.1-0.4 µg/ml in the presence of 100 ng/ml of mouse Wnt3a.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Bone Research, Signal Transduction
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: SK, DKK-1
Gene Symbol: DKK1
Molecular Weight: 25.8 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Family: Other Proteins
Protein Name: dickkopf 1 homolog (Xenopus laevis)
Protein Subtype: Cytokine Receptors
Protein Tag or Fusion: His Tag
Purity: ≥ 85% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols