Plasmid pCMV·SPORT-bgal is used as a positive control for monitoring expression in eukaryotic cells. The plasmid contains the reporter gene b-galactosidase (b-gal) from E. coli cloned as a Not I fragment into plasmid pCMV·SPORT1. The plasmid also contains a CMV promoter upstream of the b-gal gene, followed by the SV40 t-intron and polyadenylation signal. The b-lactamase gene allows selection for ampicillin resistance in E. coli.
For Research Use Only. Not for use in diagnostic procedures.