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PDPN Recombinant Human Protein, hIgG1-Fc.His Tag

Catalog number: 11065H03H25

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Description

Podoplanin (PDPN), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human PDPN short isoform (Q86YL7-1) (Met 1-Lys 123) fused to the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human PDPN (Cat: 10012-H08H) at 10 µg/ml (100 µL/well) can bind biotinylated human CLEC1B-His. The EC50 of biotinylated human CLEC1B-His is 0.71 µg/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: PDPN, CTA-520D8.1, AGGRUS, GP36, GP40, Gp38, HT1A-1, OTS8,PA2.26, T1A, T1A-2
Gene Symbol: PDPN
Molecular Weight: 38.6 kDa
Product Line: Sino Biological®
Product Size: 5 x 5 µg
Protein Family: Other Proteins
Protein Name: Podoplanin
Protein Subtype: Viral Host Cell Receptors
Protein Tag or Fusion: His Tag, IgG1 Fc Tag
Purity: ≥ 90% by SDS-PAGE
Research Category: Oncology
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols