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CDH17 Recombinant Human Protein, His Tag

Catalog number: 11360H08H5

 Related applications: Protein Assays and Analysis

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Description

Cadherin 17, LI cadherin (liver-intestine) (CDH17), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the human CDH17 (Q12864-1) extracellular domain (Met 1-Met 787) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When 5 x 104 cells/well are added to recombinant human Cadherin 17 coated plates (0.8 µg/ml with 100 µl/well), approximately >50% will adhere after 1 hour at 37°C.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: CDH16, FLJ26931, HPT-1, HPT1, MGC138218, MGC142024
Gene Symbol: CDH17
Molecular Weight: 87 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Family: Cell Structure⁄Organelle Proteins
Protein Name: cadherin 17, LI cadherin (liver-intestine)
Protein Subtype: Intercellular adhesion Molecule
Protein Tag or Fusion: His Tag
Purity: ≥ 85% by SDS-PAGE
Research Category: Development, Oncology, Other Research
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols