Invitrogen™

Dynabeads™ Untouched™ Mouse CD4 Cells Kit

Catalog number: 11415D
Invitrogen™

Dynabeads™ Untouched™ Mouse CD4 Cells Kit

Catalog number: 11415D
Catalog Number
11415D
Unit Size
2 x 10 mL
Price (USD)
Price: 820.00
Online price: 647.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
4 mL
Price (USD)
Price: 306.00
Online price: 241.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11415D2 x 10 mL
Price: 820.00
Online price: 647.65
Your price:
-
11416D4 mL
Price: 306.00
Online price: 241.65
Your price:
-
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Dynabeads™ Untouched™ Mouse CD4 Cells contains both magnetic beads and an Antibody Mix to deplete all leucocytes from mouse spleen or lymph node cells, leaving only the target mouse CD4+ cells. The negatively isolated, untouched mouse CD4+ cells are pure, viable and ready to be used in any downstream application.

• Rapid isolation of untouched mouse CD4+ T-cells – no columns required
• Pure and viable mouse CD4+ T-cells ready for any downstream application

Excellent Yield, Purity, and Viability of Recovered Cells
The Mouse Depletion Dynabeads™ suspension contains uniform, superparamagnetic beads (4.5 μm diameter) that enable easy isolation of cells from any single cell suspension. This reagent is therefore especially well-suited for enriching tissue digests such as mouse spleen and lymph node cells. The Mouse Depletion Dynabeads™ are coated with a secondary polyclonal antibody that binds rat IgGs. The Antibody Mix contains a cocktail of rat IgGs that binds mouse CD8+ T cells, B cells, NK cells, monocytes/macrophages, dendritic cells, erythrocytes and granulocytes. The Antibody Mix is first added to the sample in a tube to bind the unwanted non-CD4+ cells. The cells are then washed to remove excess antibodies, and Mouse Depletion Dynabeads™ are added. After a short incubation, the bead-bound cells are then separated from unbound cells in 1 – 2 minutes using a magnet. The purified target mouse CD4+ cells in the supernatant can be transferred to a new tube for use in any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the untouched CD4+ cells. The protocol can easily be scaled up according to your sample size.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
T Cells (CD4)
Product Type
Cell Isolation Kit
Isolation Technology
Negative Isolation
No. of Cells
Processes ∼1 x 109 cells total
Output Viability
>95%
Product Line
DYNAL™, Dynabeads™
Purity or Quality Grade
Research Grade
Quantity
2 x 10 mL
Reactivity
Mouse
Sample Type
Lymph Node, Spleen
Shipping Condition
Room Temperature
Starting Material Cell No.
1 x 107 cells per isolation
Target Species
Mouse
Diameter (Metric)
4.5 μm

Contents & Storage

Contains: 20 mL Mouse Depletion Dynabeads™ and 2 mL Antibody Mix with monoclonal antibodies towards CD8, CD11b, CD16/32, CD45R and Ter-119. These reagents are sufficient to process ∼1 x 109 cells.

Store kit components at 2°C to 8°C.

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