Invitrogen™

PureLink™ Viral RNA/DNA Mini Kit

Catalog number: 12280050
Invitrogen™

PureLink™ Viral RNA/DNA Mini Kit

Catalog number: 12280050
Catalog Number
12280050
also known as 12280-050
Unit Size
50 preps
Price (USD)
302.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12280050
also known as 12280-050
50 preps
302.00
Your Price:
***
Product Overview
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The PureLink Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants. The kit is specifically designed to isolate high-quality viral nucleic acids from a variety of RNA and DNA viruses within 45 minutes using low elution volumes that allow sensitive downstream analysis. The purified viral RNA/DNA is devoid of proteins and nucleases and is suitable for use in downstream applications that allow viral detection and genotyping.

The PureLink Viral Mini Kit has been used to purify viral dsDNA from orthopoxviruses including cowpox virus, variola virus, monkeypox virus, vaccinia virus, and HERV.

The PureLink Viral RNA/DNA Mini Kit provides the following advantages:
• Rapid and efficient purification of high-quality viral nucleic acid using spin column-based centrifugation with no sample cross-contamination
• Specifically designed to purify viral RNA and DNA from ≤500 µL cell-free samples within 45 min
• Ability to elute viral nucleic acids in low elution volumes of 10–50 µL to allow sensitive downstream analysis

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type
Spin Column
Format
Kit
Isolation Technology
Silica Spin Column
Sample Type
Cell-Free Fluids, Cerebrospinal Fluid, Liquid Samples (e.g. Serum), Plasma, Serum, Viral Samples
For Use With (Application)
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
50 Preps
Product Line
PureLink™
Product Type
Viral RNA/DNA Mini Kit
Quantity
50 Preps
Shipping Condition
Room Temperature
Target
Viral DNA⁄RNA
Test Time
45 min

Contents & Storage

The PureLink™ Viral RNA/DNA Mini Kit contains enough reagents for 50 reactions.

Kit Contents:
• 32 ml Viral Lysis buffer (L22)
• 15 ml 5X Wash Buffer (W5)
• 2 × 1.6 ml Proteinase K (20 mg/ml in storage buffer)
• 310 µg lyophilized Carrier RNA
• 15.5 ml Sterile, RNAase-free water
• 50 Viral Spin Columns with Collection Tubes
• 2 × 50 Wash Tubes (2.0 ml)
• 50 Recovery Tubes (1.5 ml)

Store all components except for Carrier RNA at room temperature. Carrier RNA should be stored at −20°C.

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