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Invitrogen™

PureLink™ Viral RNA/DNA Mini Kit

Catalog number: 12280050
Invitrogen™

PureLink™ Viral RNA/DNA Mini Kit

Catalog number: 12280050
Catalog Number
12280050
Unit Size
50 preps
Price (USD)
Price: 340.00
Online price: 272.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
1228005050 preps
Price: 340.00
Online price: 272.65
Your price:
-
Product Overview
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The PureLink Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants. The kit is specifically designed to isolate high-quality viral nucleic acids from a variety of RNA and DNA viruses within 45 minutes using low elution volumes that allow sensitive downstream analysis. The purified viral RNA/DNA is devoid of proteins and nucleases and is suitable for use in downstream applications that allow viral detection and genotyping.

The PureLink Viral Mini Kit has been used to purify viral dsDNA from orthopoxviruses including cowpox virus, variola virus, monkeypox virus, vaccinia virus, and HERV.

The PureLink Viral RNA/DNA Mini Kit provides the following advantages:
• Rapid and efficient purification of high-quality viral nucleic acid using spin column-based centrifugation with no sample cross-contamination
• Specifically designed to purify viral RNA and DNA from ≤500 μL cell-free samples within 45 min
• Ability to elute viral nucleic acids in low elution volumes of 10–50 μL to allow sensitive downstream analysis

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type
Spin Column
Isolation Technology
Silica Spin Column
Sample Type
Cells, Cerebrospinal Fluid, Plasma, Serum, Cell Culture Supernatants, Raw and Stabilized Saliva, Nasopharyngeal Viral Transfer Medium (NPVTM)
Scale
Mini
Elution Volume
10 to 50 μL
Final Product Type
Viral RNA and DNA
For Use With (Application)
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
50 Preps
Shipping Condition
Room Temperature
Test Time
45 min.
Yield
5 μg (Binding capacity)
Starting Material Amount
≤500 μL

Contents & Storage

• 310 μg Carrier RNA; –20°C
• 32 mL Viral Lysis Buffer L22; room temperature
• 15 mL Wash Buffer WII; room temperature
• 2 x 1.6 mL Proteinase K; room temperature, or 4°C for long-term storage
• 15.5 mL RNase-free Water E3; room temperature
• 50 Viral Spin Columns with Collection Tubes; room temperature
• 2 x 50 Wash Tubes; room temperature
• 50 Recovery Tubes; room temperature

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