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GFP Recombinant Aequorea victoria Protein, N-His Tag

Catalog number:  13105S07E5

 Related applications: Protein Assays and Analysis

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Description

Manufactured by Sino Biological (China); Distributed by Life Technologies.

Green Fluorescent Protein (GFP) recombinant Aequorea victoria protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein structure analysis and in cell biology research applications.

This recombinant protein is expressed from a DNA sequence encoding the Aequorea victoria GFP (AAB65663) (Ser 2-Lys 238) fused to a polyhistidine tag at the N-terminus.

N-terminal Sequence Analysis: Glu 99.

Activity:
1. Mesured by emission analysis.
2. Measure excitation and emission spectra of GFP at wavelengths of 395 nm and 510 nm respectively.

Formulation: Lyophilized from sterile PBS, pH 7.5.

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

Background
The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. The protein is in the shape of a cylinder, comprising 11 strands of β-sheet with an α-helix inside and short helical segments on the ends of the cylinder. Its ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has long been used as a universal reporter in a broad range of heterologous living cells and organisms. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms.

You may also be interested in the following GFP products:
Vectors
Primary Antibodies
Cellular Structure Probes
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Expression: E. coli
Form: Lyophilized
Gene Aliases: GFP
Gene Symbol: GFP
Molecular Weight: 28.7 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Family: Other Proteins
Protein Form: Full Length, Recombinant
Protein Name: Green Fluorescent Protein
Protein Tag or Fusion: His Tag
Purification: PAGE
Purity: ≥ 85% by SDS-PAGE
Research Category: Endocrinology
Species: Aequorea victoria
Tag Position: N-Terminal
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20°C.

Documents

Manuals & protocols