Invitrogen™

IPTG

Catalog number: 15529019
Invitrogen™

IPTG

Catalog number: 15529019
Catalog Number
15529019
Unit Size
1 g
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
155290191 g
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations

IPTG (isopropylthio-β-galactoside), a structural non-metabolizable analogue of allolactose, is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-Gal or Bluo-Gal to detect lac gene activity in cloning procedures. IPTG binds to the lac repressor, releasing it from the lac operator, and thereby allowing the transcription of genes in the lac operon, such as the gene coding for β-galactosidase.

In addition, IPTG is one of the most efficient molecular inducers for promoter’s transcriptional activity and is used extensively for heterologous protein expression induction in E. coli. Though mainly used in cell culture assays, publications demonstrated the use IPTG in vivo in animal models too.

Primary applications
• Blue-white screening—induce functional β-galactosidase
• Induce protein expression from inducible T7 RNA polymerase E. coli expression systems egulated by lac operon

Find the media format that fits your needs
We offer a wide variety of ready-to-use media formulations, such as LB Broth, Terrific Broth or S.O.C recovery medium.
X-Gal or Bluo-Gal for blue-white screening.
Explore other media formats for bacterial growth.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Name or Material
IPTG (Isopropyl β-D-thiogalactopyranoside)
Format
Bottle
Quantity
1 g
Shipping Condition
Approved for shipment at Room Temperature or on Wet Ice
Form
Powder

Contents & Storage

• IPTG Powder (1 g)

Store at –20°C.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text