Invitrogen™

Platinum™ Taq DNA Polymerase, DNA-free

Catalog number:  15966005

 Related applications:

DNA Sequencing

| PCR

| Real Time PCR (qPCR)

| Sanger Sequencing

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Description

Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA. This enzyme is an ideal choice for PCR-based applications requiring the highest sensitivity without false-positive results from reagent-borne contamination. This DNA-free enzyme provides the same high level of performance and lot-to-lot consistency as standard Platinum Taq DNA Polymerase.

DNA-free Platinum Taq DNA Polymerase offers:
• Certified low-DNA contamination level (≤0.01 copy of bacterial gDNA per enzyme unit)
• Confidence in qualitative and quantitative detection of low-abundance microorganisms due to signal absence with no-template/reagents-only PCR controls
• Same performance as Platinum Taq DNA Polymerase manufactured using conventional process

Platinum Taq DNA Polymerase, DNA-free, is a recombinant Taq polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures and dissociates after the initial denaturation step at 94°C. This automatic “hot start” provides increased sensitivity, specificity, and yield, while allowing convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase, DNA-free, has a non-template–dependent terminal transferase activity that adds a 3′ deoxyadenosine to product ends, and has a 5′→3′ exonuclease activity.

Use Platinum Taq DNA Polymerase, DNA-free, for the amplification of DNA from complex genomic and viral templates, as well as in RT-PCR. It is the perfect choice for PCR assays where higher sensitivity and specificity are needed to minimize ambiguous or false-positive results. Find out more at www.thermofisher.com/dna-free.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: Platinum™ Taq DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1
GC-Rich PCR Performance: Low
Reaction Format: Standalone
Product Overhang: 3'-A
Reaction Speed: Standard
Product Line: Platinum™
Product Size: 500 U
Shipping Condition: Dry Ice

Contents & storage

• Platinum Taq DNA Polymerase, DNA-free, 100 µL
• 10X PCR Buffer (-MgCl2), DNA-free, 1.25 mL
• 50 mM MgCl2, DNA-free, 1 mL

Store at -5 to -30°C in a non-frost-free freezer.

Documents

Manuals & protocols