Invitrogen™
Platinum™ Taq DNA Polymerase, DNA-free
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Description
Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA. This enzyme is an ideal choice for PCR-based applications requiring the highest sensitivity without false-positive results from reagent-borne contamination. This DNA-free enzyme provides the same high level of performance and lot-to-lot consistency as standard Platinum Taq DNA Polymerase.DNA-free Platinum Taq DNA Polymerase offers:
• Certified low-DNA contamination level (≤0.01 copy of bacterial gDNA per enzyme unit)
• Confidence in qualitative and quantitative detection of low-abundance microorganisms due to signal absence with no-template/reagents-only PCR controls
• Same performance as Platinum Taq DNA Polymerase manufactured using conventional process
Platinum Taq DNA Polymerase, DNA-free, is a recombinant Taq polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures and dissociates after the initial denaturation step at 94°C. This automatic “hot start” provides increased sensitivity, specificity, and yield, while allowing convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase, DNA-free, has a non-template–dependent terminal transferase activity that adds a 3′ deoxyadenosine to product ends, and has a 5′→3′ exonuclease activity.
Use Platinum Taq DNA Polymerase, DNA-free, for the amplification of DNA from complex genomic and viral templates, as well as in RT-PCR. It is the perfect choice for PCR assays where higher sensitivity and specificity are needed to minimize ambiguous or false-positive results. Find out more at www.thermofisher.com/dna-free.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Polymerase: | Platinum™ Taq DNA Polymerase |
|---|---|
| Hot Start: | Built-In Hot Start |
| Fidelity (vs. Taq): | 1 |
| GC-Rich PCR Performance: | Low |
| Reaction Format: | Standalone |
| Product Overhang: | 3'-A |
| Reaction Speed: | Standard |
| Product Line: | Platinum™ |
| Product Size: | 500 U |
| Shipping Condition: | Dry Ice |
Contents & storage
• Platinum Taq DNA Polymerase, DNA-free, 100 µL
• 10X PCR Buffer (-MgCl2), DNA-free, 1.25 mL
• 50 mM MgCl2, DNA-free, 1 mL
Store at -5 to -30°C in a non-frost-free freezer.
• 10X PCR Buffer (-MgCl2), DNA-free, 1.25 mL
• 50 mM MgCl2, DNA-free, 1 mL
Store at -5 to -30°C in a non-frost-free freezer.
