ElectroMAX™ DH10B™ Cells

Description

ElectroMAX™ DH10B™ Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 10¹⁰ cfu/µg plasmid DNA. ElectroMAX™ DH10B™ Cells are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. ElectroMAX™ DH10B™ Cells provide:

• High-efficiency transformation to maximize clones
• Enhanced genetic markers for cDNA or gDNA cloning capabilities

Highest transformation efficiencies
ElectroMAX™ DH10B™ Cells offer the highest transformation efficiencies available with >1 x 10¹⁰ transformants/µg of control DNA in a 20 µL reaction. These high transformation efficiencies make ElectroMAX™ DH10B™ Cells ideal for efficient cloning of both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes. ElectroMAX™ DH10B™ Cells are also suitable for construction of gene banks, for generation of cDNA libraries using plasmid-derived vectors, and for situations when there is limited amount of DNA.

Note: A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm is required.

Versatile cloning capabilities
ElectroMAX™ DH10B™ Cells have the following features:

• Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-gal or Bluo-gal
• mcrA genotypic marker and the mcrBC, mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine
• endA1 mutation enables increased plasmid yield and quantity

Genotype: F^-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ^-rpsL nupG

Find the strain and format that you need
DH10B™ cells are available in both electrocompetent and chemically competent formats. Our ElectroMax™ DH10B™ T1R Cells and MegaX DH10B™ T1^R Electrocomp Cells offer the benefit of T1 and T5 phage resistance.

Specifications

Competent Cell Type:	Electrocompetent
Species:	E. coli
Format:	Tube(s)
Transformation Efficiency:	> 1x10^10
Bacterial or Yeast Strain:	DH10B™
Product Line:	DH10B™, ElectroMAX™
Blue-White Screening:	Yes
Cloning Methylated DNA:	Yes
Cloning Unstable DNA:	Not Suitable for cloning unstable DNA
F' Episome (to make ssDNA):	Lacks F' Episome
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Improves Plasmid Quality:	Yes
Plasmid Type:	May be used for plasmids > 20 kb
Preparing Unmethylated DNA:	Not Suitable for preparing unmethylated DNA
Product Size:	0.5 mL
Propagating ccdB Vectors:	Not for ccdB vector propagation
Reduces Recombination:	Yes
T1 Phage - Resistant (tonA):	No
Shipping Condition:	Dry Ice

Contents & storage