ElectroMAX DH10B Cells are electrocompetent
E. coli cells offering the highest transformation efficiencies of >1 x 10
10 cfu/µg plasmid DNA. ElectroMAX DH10B Cells are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. ElectroMAX DH10B Cells provide:
• High-efficiency transformation to maximize clones
• Enhanced genetic markers for cDNA or gDNA cloning capabilities
Highest transformation efficiencies ElectroMAX DH10B Cells offer the highest transformation efficiencies available with >1 x 10
10 transformants/µg of control DNA in a 20 µL reaction. These high transformation efficiencies make ElectroMAX DH10B Cells ideal for efficient cloning of both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes. ElectroMAX DH10B Cells are also suitable for construction of gene banks, for generation of cDNA libraries using plasmid-derived vectors, and for situations when there is limited amount of DNA.
Note: A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm is required.
Versatile cloning capabilitiesElectroMAX DH10B Cells have the following features:
• Φ80
lacZΔM15 marker provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-gal or Bluo-gal
•
mcrA genotypic marker and the
mcrBC,
mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine
•
endA1 mutation enables increased plasmid yield and quantity
Genotype: F
-mcrA Δ(
mrr-
hsdRMS-
mcrBC) Φ80
lacZΔM15 Δ
lacX74
recA1
endA1
araD139Δ(
ara,
leu)7697
galU
galK λ
-rpsL
nupG
Find the strain and format that you needDH10B cells are available in both electrocompetent and chemically competent formats. Our
ElectroMax DH10B T1R Cells and
MegaX DH10B T1R Electrocomp Cells offer the benefit of T1 and T5 phage resistance.
For Research Use Only. Not for use in diagnostic procedures.