Invitrogen™

ElectroMAX™ DH10B Cells

Catalog number: 18290015
Invitrogen™

ElectroMAX™ DH10B Cells

Catalog number: 18290015
Catalog Number
18290015
Unit Size
5 x 100 µL
Price (USD)
336.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
182900155 x 100 µL
336.00
Your Price:
***
Product Overview
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ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/µg plasmid DNA. ElectroMAX DH10B Cells are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. ElectroMAX DH10B Cells provide:

• High-efficiency transformation to maximize clones
• Enhanced genetic markers for cDNA or gDNA cloning capabilities

Highest transformation efficiencies
ElectroMAX DH10B Cells offer the highest transformation efficiencies available with >1 x 1010 transformants/µg of control DNA in a 20 µL reaction. These high transformation efficiencies make ElectroMAX DH10B Cells ideal for efficient cloning of both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes. ElectroMAX DH10B Cells are also suitable for construction of gene banks, for generation of cDNA libraries using plasmid-derived vectors, and for situations when there is limited amount of DNA.

Note: A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm is required.

Versatile cloning capabilities
ElectroMAX DH10B Cells have the following features:

• Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-gal or Bluo-gal
mcrA genotypic marker and the mcrBC, mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine
endA1 mutation enables increased plasmid yield and quantity

Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ-rpsL nupG

Find the strain and format that you need
DH10B cells are available in both electrocompetent and chemically competent formats. Our ElectroMax DH10B T1R Cells and MegaX DH10B T1R Electrocomp Cells offer the benefit of T1 and T5 phage resistance.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Competent Cell
Contains F' Episome
Lacks F' Episome
Efficiency
High Efficiency (>10^9 cfu/µg)
Cell Type
Electrocompetent
Improves Plasmid Quality
Yes
Libraries
cDNA or gDNA
Cloning Methylated DNA
Yes
Transformation Efficiency Level
High Efficiency (> 10^9 cfu⁄µg)
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Bacterial or Yeast Strain
DH10B
Blue/White Screening
Yes
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Plasmid
May be used for plasmids > 20 kb
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
Yes
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
No
Species
E. coli
Format
Tube
Product Line
DH10B, ElectroMAX™

Contents & Storage

Contains:
• ElectroMAX DH10B Cells: 5 vials, 100 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

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