CaptureSelect™ IgG-CH1 Affinity Matrix has been specifically designed for the purification of recombinant human Fab fragments and IgGs, recognizing all IgG subclasses (1, 2, 3 and 4) independent of the light chain subclass (Kappa/Lambda).
Features of this affinity matrix include:
• Platform approach for purification of IgGs and their Fab fragments • No co-purification of overexpressed free lights chains due to heavy chain binding • Excellent scalability • Non-animal-derived
CaptureSelect™ IgG-CH1 Affinity Matrix purifies IgG, Fab, and Fab2 fragments directly from complex source materials in a single step with high purity and yield.
Free of Animal Components Our CaptureSelect™ products are affinity ligands created by a proprietary technology based on Camelid-derived single domain antibody fragments. The ligand is a 14 kDa single domain fragment comprising the 3 CDRs that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.
Matrix: agarose-based, aldehyde-activated Average particle size: 65 ± 10 µm Ligand: CaptureSelect™ IgG-CH1 affinity ligand Ligand coupling method: aldehyde coupling via NH2 residues of the ligand Binding capacity: 25–30 g IgG/L resin depending on flow rate, column height and contact time Elution conditions: 20 mM citric acid, 150 mM NaCl, pH 3.5 Flow characteristics: 200–400 cm/h Formulation buffer: 20%(v/v) ethanol
Caution: For manufacturing, processing, or repacking.