Pierce™ Glycoprotein Staining Kit

Catalog number: 24562

Thermo Scientific™  Related applications: Protein Gel Electrophoresis

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Description

The Thermo Scientific Pierce Glycoprotein Staining Kit is a convenient, fast and sensitive kit for staining glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method.

Features of the Glycoprotein Staining Kit:

Specific for glycoproteins—periodic acid-Schiff (PAS) method specifically detects glycosylated proteins having sialic acid and other oxidizable carbohydrate groups
Optimized protocol—the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours (half the time required for other staining methods)
Colorimetric—glycoproteins are detected as magenta bands against a faint pink or colorless gel background
Qualitative staining—detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625ng avidin and 0.16 µg horseradish peroxidase per band
Controls included—complete kit includes positive and negative control proteins (HRP and STI, respectively).
Gels or membranes—effective for use with polyacrylamide mini gels or nitrocellulose membranes

The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins (glycoproteins) that have been separated by polyacrylamide gel electrophoresis (PAGE). A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.

To use the Pierce Glycoprotein Staining Kit, simply treat the gel or membrane containing separated proteins treated with the provided periodate-based Oxidation Reagent. This treatment oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are reacted with the Pierce Glycoprotein Stain Reagent to form Schiff-base bonds that result in the production of magenta bands of the stain. Traditionally called the periodate-acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Target Molecule: Proteins (Glycoproteins)
Label or Dye: Fuchsin Sulfite
Detection Method: Colorimetric
Detection Location: In-Gel Detection, In-Blot Detection
Product Size: 750 mL

Contents & storage

Sufficient For: 10 mini gels (SDS-PAGE)
• Oxidation Reagent, 2.5 g powder to make 250 mL
• Reduction Reagent, 1.25 g powder to make 250 mL
• Positive Control (Horseradish Peroxidase), 1 mg
• Negative Control (Soybean Trypsin Inhibitor), 1 mg
• Glycoprotein Stain, 250 mL

Store all components at 4°C.

Documents

Manuals & protocols

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