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dRhodamine Dye Terminator Ready Reaction Cycle Sequencing Kit

Catalog number: 4303143

Applied Biosystems™  Related applications: DNA Sequencing | Sanger Sequencing

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Description

The dRhodamine Terminator Cycle Sequencing Kit provides optimized dRhodamine dideoxy terminator chemistries for decreased background noise in a wide array sequencing applications.
• Increase your signal-to-noise ratio.
• Simplify workflows with easy-to-clean-up chemistries.
• Get optimized chemistry for sequencing templates with homo-polymers (AA's, TT's, etc.).
• Obtain clean signal, even peak patterns, and minimal mobility shifts with improved dye terminators.
• Reduce spectrum overlap with narrow dye emission spectra.

Simplify Your Sequencing Workflow
This kit's dRhodamine-dye terminator-based cycle sequencing enables you to sequence DNA rapidly and conveniently. Dye terminators allow you to set up reactions in a single tube, reduce pipetting and liquid handling, and increase sample throughput.

Higher Accuracy, Longer Read Lengths
The dRhodamine Terminator Cycle Sequencing Kit contains the same components as the standard BigDye® Terminator Kit, except dRhodamine (dichlororhodamine) replaces rhodamine terminators, so you get more even peak patterns and less background noise. This alternative chemistry ensures you get higher accuracy, longer read lengths, and more reliable automated sequencing.

Easy to Integrate into Your Workflow
The kit's chemistry requires no new software or instrument recalibration, so you can easily integrate it into your current workflow with minimal changes to your protocols.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): 310 Genetic Analyzer, 3130xl Genetic Analyzer, ABI PRISM™ 377, GeneAmp® 2700, GeneAmp® 9600, GeneAmp™ 2400, GeneAmp™ 9700
Recommended Applications: Standard Sequencing
Format: 8-well strips, 96-well plate
Product Line: Applied Biosystems™
Product Size: 5,000 reactions
Technique: Fluorescent Dye Terminator Sequencing
Template Compatibility: PCR Amplicons, Plasmid DNA (≤15Kb), Single Stranded DNA

Contents & storage

Each kit contains dRhodamine Terminator Ready Reaction Mix, M13 (-21) Primer, pGEM Control DNA, and a user's manual. Store kit at -15 to -25°C and avoid repeated freeze-thaw cycles.

Documents

Manuals & protocols