SNaPshot® Multiplex Kit, with protocol

Catalog number: 4323151

Applied Biosystems™  Related applications: DNA Sequencing | Fragment Analysis | Genotyping & Genomic Profiling | Sanger Sequencing

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Description

The SNaPshot® Multiplex System is a primer extension-based method that enables multiplexing up to 10 SNPs (single nucleotide polymorphisms). Use this system to screen and confirm SNPs, assess DNA methylation, fingerprint BACs, and screen samples for susceptibility to scrapie.

• Perform multiplexed, single-base extension of up to 10 SNPs.
• Interrogate multiple SNPs regardless of chromosome position.
• Separate SNP loci that differ by a single base pair.
• Perform robust interrogation of multiple SNPs with only a small amount of amplified template.
• Decrease expenses with this moderately priced system.
• Reduce the complexity of your experiments with this well-tested, easy-to-understand single-base extension technique.

Screen and Confirm SNPs
The SNaPshot® Multiplex System is the perfect tool for SNP screening and validation. The kit offers a one-tube single-base extension⁄termination reagent to label DNA fragments. First, run DNA fragments on any Applied Biosystems capillary electrophoresis instrument with the GeneScan™-120 LIZ® Size Standard to determine the size of labeled fragments. Then use GeneMapper® Software to analyze the data and generate allele calls. The SNaPshot® Multiplex Kit allows multiplexing during single-base extension of up to 10 primer-template combinations in a single tube-single capillary format.

Assess DNA Methylation
The study of methylation⁄epigenetics is emerging as an important component of cancer research. In a typical assay to detect methylation, bisulfite treatment of DNA deaminates non-methylated cytosine and converts it to uracil while methylated cytosine remains unreactive. The subsequent step of PCR amplification coverts uracil bases to thymine. Use SNaPshot® to quantitatively detect the base differences in treated and untreated samples to learn the methylation status of your samples.

Fingerprint BACs
New BAC libraries require a rapid, efficient method for characterization (also called fingerprinting) and assembly of clones into contigs (contiguous consensus sequences), which are then arrayed into physical maps of the chromosome. Use the SNaPshot® Multiplex Kit to fingerprint clones by labeling BAC fragments after digestion with restriction endonucleases. The labeled fragments can then be separated and detected on any Applied Biosystems capillary electrophoresis instrument. Sizing information from GeneMapper® Software v3.5 or higher is imported into subsequent editing and contig assembly programs. The high-quality results you get from the SNaPshot® Multiplex Kit provide an easy-to-use and cost-effective solution for high-throughput BAC fingerprinting.

Screen Samples for Susceptibility to Scrapie
Scrapie, like other prion diseases are caused by abnormally-folded isoforms of host-encoded proteins. The single base-pair sensitivity of the SNaPshot® system enables you to screen samples accurately for codon differences in prion genes. Use the SNaPshot® Multiplex System to screen for SNPs in the genes that code for these proteins. For instance, polymorphisms at codons 136, 154 and 171 of the PrP gene in sheep and goats can lead to abnormally folded isoforms of the protein product to result in scrapie.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: All Species
Technique: Primer Extension, SNP Genotyping (Fragment Analysis-Based)
Product Size: 100 reactions
Detection Method: Capillary Electrophoresis
Genotyping Target: SNPs (Known)
Sample Type (General): DNA
For Use With (Equipment): 3500xL Genetic Analyzer, 3730 DNA Analyzer, 3730xl DNA Analyzer, 310 Genetic Analyzer, 3100-Avant™ Genetic Analyzer, 3130xl Genetic Analyzer, 3500 Genetic Analyzer

Contents & storage

Contains:
• SNaPshot® Multiplex Ready Reaction Mix (for 100 reactions)
• SNaPshot® Multiplex Control Primer Mix (30 µl)
• SNaPshot® Multiplex Control Template (60 µl)
• Protocol

Store at -15°C to -25°C in a constant-temperature freezer.

Documents

Manuals & protocols