Applied Biosystems™

7500 Real-Time PCR System, desktop

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The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

• Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄ TAMRA™⁄ Cy3™, ROX™⁄Texas Red®, and Cy5™ dyes
• Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets
• Advanced multi-componenting algorithm minimizes spectral crosstalk – superior for multiplexing
• User-friendly software includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward

NEW - 7500 Software v2.x
Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.x upgrade. The 7500 Software v2.x incorporates your favorite StepOne Software features, such as a variety of plate setup wizards, standard curve dilution and master mix recipe calculators, QC flags, data filters, and email notification when a run is finished. The 7500 Software v2.x also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates.

The NEW Gene Expression Study package accommodates large studies better than any other instrument software package:
• Import an unlimited number of Comparative CT (relative quantitation) files to one study
• Group samples and view data both by technical replicate group and biological replicate group
• Use any gene(s) as an endogenous control, including averaging multiple controls together
• Enter known efficiency values to be factored into the RQ results

21 CFR Part 11 Module available
The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings.
• Individual user log-ins can be added for up to four user groups, each group with designated permission settings
• User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts
• Audit trails can be enabled or disabled depending on your traceability needs
• A selection of e-signatures is available to ease e-signatures into your workflow

Supports Many Applications
Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +⁄– assays utilizing internal positive controls. To facilitate many of these applications, Applied Biosystems® provides preformulated, ready-to-use, quality-tested, TaqMan® assays for use with the 7500 system. Now you can reduce your assay optimization efforts.

Upgrade to High-Speed Thermal Cycling
An optional upgrade to the 7500 Fast System is available. This 7500 Fast System uses our master mix formulations and enables you to shorten your real-time PCR runs to as little as 30 minutes.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


Calibrated Dye: SYBR® Green I, ROX™, NED™, TAMRA™, JOE™, Texas Red®, Cy5™, VIC™, FAM™, Cy3™
Dimensions: Width: 34 cm (13.39 in), Depth: 45 cm (17.72 in), Height: 49 cm (19.29 in)
External Computer: 80 GB HD, Dell Minitower: Celeron 346 (3.06 GHz), 1 GB NON ECC 533 MHz DDR2, 16X DVD
Format: 96-well plate
High-throughput Compatibility: Multiplexing
Max. Power: 950 W
Optics: CCD Camera, 5 excitation filters, Tungsten-halogen lamp, 5 emission filters
Passive Reference Dye: ROX (Separate Tube), No ROX, ROX (Pre-mixed)
Peak Block Ramp Rate: 2.5°C⁄sec
Precision: 99.7% confidence (5000 & 10000 copies)
Reaction Speed: Standard
Reaction Volume Range: 20-100 µl
Sample Ramp Rate: ± 1.1°C⁄sec
Software Compatibility: Primer Express, 21 CFR Part 11 Module (optional), 7500 System Software (RQ included)
Temperature Accuracy: 0.25°C (35°C to 95°C after 3 min)
Temperature Range (Metric): 4-100°C
Temperature Uniformity: 0.50°C (after 30 sec)
Thermal Cycling System: Peltier-Based System
Throughput: 4 to 5-96-well plates per 8-hr day
Weight: 75 lbs
For Use With (Equipment): 7500 System
Includes: Desktop Computer
Dynamic Range: 9 logs
Sensitivity: 1 copy of RNase P gene (human genomic DNA)
Voltage: 120V

Contents & storage