Applied Biosystems™

StepOnePlus™ Real-Time PCR System Spectral Calibration Kit (96 well)

Catalog number:  4371435

 Related applications:

Real Time PCR (qPCR)

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This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR® Green I, VIC®/JOE™, and NED™/TAMRA™/ROX™ dyes on the StepOnePlus™ Real-Time PCR System.

The Applied Biosystems® StepOnePlus™ Real-Time PCR System Spectral Calibration Kit contains one background plate and two preloaded, 96-well fast optical reaction plates containing seven dye standards.
• 96-well optical reaction plates preloaded with dyes (FAM™/SYBR® Green I, VIC®/JOE™, NED™/TAMRA™/ROX™) are provided for quick and easy calibration of the StepOnePlus™ Real-Time PCR System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research—Not Instrument Calibration
This kit's easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the StepOnePlus™ Real-Time PCR System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.
For Research Use Only. Not for use in diagnostic procedures.


Calibration Type: Spectral Calibration
Detection Method: Fluorescent
For Use With (Equipment): StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode
Label or Dye: FAM, JOE, NED, ROX, SYBR® Green, TAMRA™, VIC
Product Line: StepOnePlus™
Product Size: 1 kit
Shipping Condition: Dry Ice

Contents & storage

Includes one background plate and two 96-well fast reaction plates containing spectra dyes (plates I & II). Plate I contains FAM, VIC, NED, and ROX; Plate II contains SYBR Green I, JOE, and TAMRA.

Store at -15°C to -25°C. Protect from light.


Manuals & protocols