StepOnePlus™ Real-Time PCR System Spectral Calibration Kit (96 well)
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Applied Biosystems™

StepOnePlus™ Real-Time PCR System Spectral Calibration Kit (96 well)

This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM/SYBR Green I, VIC/JOE, NED/TAMRA, and ROX dyesRead more
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Catalog NumberQuantity
43714351 Kit
Catalog number 4371435
Price (CLP)
-
Quantity:
1 Kit
This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM/SYBR Green I, VIC/JOE, NED/TAMRA, and ROX dyes on the StepOnePlus Real-Time PCR System.

The Applied Biosystems StepOnePlus Real-Time PCR System Spectral Calibration Kit contains one background plate and two preloaded, 96-well fast optical reaction plates containing seven dye standards.
• 96-well optical reaction plates preloaded with dyes (FAM/SYBR Green I, VIC/JOE, NED/TAMRA, ROX) are provided for quick and easy calibration of the StepOnePlus Real-Time PCR System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research—Not Instrument Calibration
This kit's easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the StepOnePlus Real-Time PCR System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Calibrated DyeFAM, JOE, NED, ROX, TAMRA™, VIC, SYBR™ Green
Calibration TypeSpectral Calibration
Detection MethodFluorescence
For Use With (Application)Real Time PCR (qPCR)
For Use With (Equipment)Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode
Product LineStepOnePlus
Quantity1 Kit
Shipping ConditionDry Ice
Product TypeSpectral Calibration Kit
Unit SizeEach
Contents & Storage
Includes one background plate and two 96-well fast reaction plates containing spectra dyes (plates I and II). Plate I contains FAM, VIC, NED, and ROX; Plate II contains SYBR Green I, JOE, and TAMRA.

Store at -15°C to -25°C. Protect from light.

Frequently asked questions (FAQs)

What type of Real-Time PCR (Sequence Detection System) information is available from Applied Biosystems?

Tutorials demonstrating introductions to Real-Time PCR, guidelines for designing real-time assays using Primer Express software, and various instrument set-ups can be found under Life Technologies University on our website. User bulletins, manuals and Product inserts, protocols can be found as downloadable pdf files on our Technical Resources page.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.