Protein Thermal Shift™ Starter Kit

Catalog number: 4462263

Protein Thermal Shift™ Starter Kit

Catalog number: 4462263
Catalog Number
Unit Size
1,000 reactions
Price (USD)
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
44622631,000 reactions
Your Price:
Product Overview
Citations & References
Additional Information
Applied Biosystems® Protein Thermal Shift™ kits allow users to employ a dye that binds to exposed hydrophobic residues to monitor the thermal stability of proteins using a real-time PCR instrument in order to identify buffer conditions that stabilize the protein of interest or screen libraries of ligands for compounds that bind the protein of interest.

Save Time and Money with Your Sample Screening
This solution significantly reduces the time and cost associated with screening samples relative to currently available methods. Protein Thermal Shift™ products that are run on our real-time PCR systems enable researchers to inexpensively and efficiently:

• Screen samples for relative protein thermal stability
• Screen molecules (e.g. small molecule drug candidates, fragment-based libraries, and antibodies) for ligand binding to proteins in a high-throughput manner
• Screen samples for stability changes after protein point mutations or modifications
• Systematically identify suitable⁄optimal buffer conditions for the measurement of protein-ligand interactions, production of stable protein preparations, protein crystallization, and storage of proteins
• Quickly QC protein preps to ensure protein integrity and⁄or purity

Two Kits to Choose From
The Protein Thermal Shift™ Dye Kit contains the dye and buffer for performing protein thermal shift experiments, enough for up to 1000 reactions. The Protein Thermal Shift™ Starter Kit contains the Dye Kit plus a control protein and control ligand for test experiments and troubleshooting. The Protein Thermal Shift™ Starter Kit components are packaged in two boxes, one to be stored at -20°C and the other at 4°C, for maximum flexibility in reagent formulation.

Simplify Analysis with Protein Thermal Shift™ Software
Our complete Protein Thermal Shift™ solution includes analysis software which is compatible with run files generated from five of our real-time PCR systems: StepOne™ Real-Time PCR System, StepOnePlus™ Real-Time PCR system, 7500 Fast Real-Time PCR System, and ViiA™7 Real-Time PCR System.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Application)
Pharma, Biopharma, Real Time PCR (qPCR)
For Use With (Equipment)
7500 Fast System, 7500 System, StepOne™, StepOnePlus™, ViiA™ 7 System
Kit Type
Starter Kit
Product Line
Protein Thermal Shift™
Target Specificity
Not Target-Specific
Detection Method
No. of Reactions
1000 Reactions
PCR Method
qPCR Protein Thermal Stability Analysis
Sample Type
Fluorescence Intensity

Contents & Storage

Store dye and buffer at 18–25°C; store control ligand and control protein at -15°C to -25°C.


Frequently asked questions (FAQs)

Citations & References