Ion Personal Genome Machine® (PGM™) System

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


The new Personal Genome Machine (PGM™) System combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data, democratizing sequencing and making it accessible to virtually any lab or clinic. The system leverages the exponential improvements in the semiconductor industry (known as Moore’s Law) to provide scalability and flexibility for various applications. The system’s use of the simplest, natural sequencing chemistry eliminates the need for expensive optics and reduces complex chemistries to measure natural DNA extension. Direct, real time sequencing detection provides sequencing results typically in less than 3 hours.

Affordable Sequencing For Nearly Every Lab
Previous sequencing technologies have relied upon optical systems as bridges between the chemical and digital forms of sequence information. These systems typically entail significant additional cost. By eliminating the optical system, the Personal Genome Machine™ provides sequencing that is simpler, faster, more cost effective, and more scalable. With a small benchtop footprint, weight of about 65 lbs (30 kg), low cost sequencing reagents, and a simple touchscreen user interface, the Personal Genome Machine™ brings next generation sequencing to nearly every lab. The Torrent Server is an integral part of the Personal Genome Machine (PGM™) System, converting raw signal into base calls typically in less than 1 hour. The Torrent Suite Software performs preliminary reference genome alignment and outputs data in FASTQ and SFF formats for easy downstream analysis with optional third party analysis tools. It can also provide access to a web portal for data review and sharing.

Torrent Server Features Typically Include:
• Processors - Two Six-core processors
• RAM - 48 GB RAM
• HDD Capacity - Eight 2 TB Hard drives in RAID 5 with 12 TB usable
• Network - Quad port gigabit NIC
• GPU - NVIDIA Graphic Processor Unit
• Chassis - Dell Precision T7500 tower. No rack mount available.
• Monitor⁄Keyboard - not included - file access available via SSH or web service

Ion Power: PostLight™ Sequencing Produces Bits From Bases
The sequencing technology underlying the Personal Genome Machine (PGM™) exploits a well-characterized biochemical process: When a nucleotide is incorporated into a strand of DNA by a polymerase, a hydrogen ion (H+) is released as a byproduct. This hydrogen ion carries a charge which the PGM™ System’s ion sensor-essentially the world's smallest solid-state pH meter- can detect. As the sequencer floods the chip with one nucleotide after another, any nucleotide added to a DNA template will be detected as a voltage change, and the PGM™ System will call the base. If a nucleotide is not a match for a particular template, no voltage change will be detected and no base will be called for that template.

Massively Parallel Sequencing On Your Bench
A principal component of the Personal Genome Machine™ System is the sequencing chip. This microprocessor chip incorporates an extremely dense array of >1 million micro-machined wells married to our proprietary ion sensor. Each well contains a different DNA template, allowing massively parallel sequencing. Chips can scale in density for any application, from small, targeted experiments to large genomes.

The Right Tool To Get Results Easily, Reliably, and Quickly
Because it detects nucleotide incorporation without the use of light, the Personal Genome Machine™ System uses the simplest sequencing chemistry possible-natural nucleotides. There is no need for expensive and error-prone modified bases, enzymatic cascades, chemiluminescence, or fluorescence. Direct detection also means the incorporation of each nucleotide is recorded in seconds. As a result, you can do an entire run typically in about 2 hours. Library preparation methods currently used for next-generation sequencing can be used with the Personal Genome Machine™.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


CPU: Dual 6-core 2.66 GHz CPUs
Output: Ion 314™ Chip v2: Up to 100 Mb, Ion 316™ Chip v2: Up to 1 Gb, Ion 318™ Chip v2: Up to 2 Gb
Weight: 65 lbs (30 kg) (Freestanding)
Capacity: 1 chip per run
Run Time: Ion 318™ Chip v2: 4.4 to 7.3 hr, Ion 314™ Chip v2: 2.3 to 3.7 hr, Ion 316™ Chip v2: 3.0 to 4.9 hr
Frequency: 50⁄60 Hz
Technique: Post-Light™ Ion Semiconductor Sequencing
Dimensions: 24 in (61 cm) (W) x 20 in (51 cm) (D) x 21 in (53 cm) (H)
Throughput: Up to 2 Gb per run
Data Output: VCF Files, BAM Files, FASTQ Files, SFF Files
Read Length: 35–400 bases (average: 200 bases)
Product Size: 1 instrument
Current Limit: 6.7 A (max)
Voltage Limit: 100 V (min) to 240 V (max)
Reads⁄Run: Ion 318™ Chip v2: 4-5.5 million, Ion 314™ Chip v2: 400-500 thousand, Ion 316™ Chip v2: 2-3 million
Operating System: Ubuntu 10.04
Sequencing Scale: 3 Mb to 2 Gb
Display Interface: Touchscreen User Interface
External Computer: Torrent Server
Instrument Memory: Eight 2 TB Hard drives in RAID 5, 48 GB RAM
Chip Compatibility: Ion 314™ Chip v2 BC, Ion 318™ Chip v2 BC, Ion 316™ Chip v2 BC
For Use With (Equipment): Ion PGM™ System
High Throughput Compatibility: High Throughput-Compatible, Multiplexing

Contents & storage

This system includes the Ion PGM™ Sequencer and the Torrent Server with Torrent Suite Software. A one-year warranty covering parts and labor is included.


Manuals & protocols